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The map label for this gene is sigB

Identifier: 227816152

GI number: 227816152

Start: 3252085

End: 3252858

Strand: Reverse

Name: sigB

Synonym: BAMEG_3579

Alternate gene names: 227816152

Gene position: 3252858-3252085 (Counterclockwise)

Preceding gene: 227816153

Following gene: 227816151

Centisome position: 62.19

GC content: 36.05

Gene sequence:

>774_bases
ATGGAAATCCAATCTCAACCTACGAATCTTACTAAAGAAGACGTTATTAAACTAATTGCAGAATTTCAACAAAACCAATG
TGGTGAAGCGCAGGAAAGGTTAGTTGATCATTATAAAAATCTCGTATATTCCATTGCATATCGCTATTCAAAAGGCGGGC
CAATGCATGAGGATATTATACAAGTAGGCATGTTAGGGCTCTTAGGTGCAATAAGAAGGTATGATTATTCGATAGGGAAT
GCTTTTGAGCCTTTTGCAATACCTACGATAGTAGGGGAAATAAAGAAGTATTTACGCGATAAAACTTGGGGCATTCACGT
TCCAAGGCGAATTAAAGATTTAGGCGGAAAAATTAAACTTGCGATAGAGGAACTAACAGATCATTTGCAGCGTTCACCGA
AGATTATAGAGATTGCGGATCATTTAGGACTATCCGAAGAGGAAGTGCTAGAGATTATGGATGCGAAAAATAATTATCGA
GTATCTTCCTTAGATGATGTAGTTGAAAATGCATCTGATGGCAGTTCGGTAGCGAGAATTGAATCTGTAGGTGAAGTAGA
GCAAGGATATGAACAGACAGAAAGGCGTCTCGTTTTAAAGGATATTTTTAACGTATTAAATGAAACGGAAAAGAGTGTTA
TTCATTATATATTTGAAGAAAATTTAAATCAAAAAGATACAGGGGAACGGTTAGGTATTTCACAAATGCACGTTTCTCGT
ATTAAAAGACAAGCGATAAGTAAGTTGAAACAAGCCGCATTTTTAGATACATAA

Upstream 100 bases:

>100_bases
TATTTAATCAATACGTTAATGGATGATATACAAATCATGCATGATGAGGGCATGACGGTTTTAATGACAAAATATATACA
AAGAGAGCAGGTGGAGAATG

Downstream 100 bases:

>100_bases
AAAATTTAAAATAATGATGTTTAAAACATGAGAAAAGGGGTACTTATTAAGTATAGGAGGAGATATGAAATGTCACACGA
TGTGAAAGAACTAATCGAAG

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MEIQSQPTNLTKEDVIKLIAEFQQNQCGEAQERLVDHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDHLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEQTERRLVLKDIFNVLNETEKSVIHYIFEENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT

Sequences:

>Translated_257_residues
MEIQSQPTNLTKEDVIKLIAEFQQNQCGEAQERLVDHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDHLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEQTERRLVLKDIFNVLNETEKSVIHYIFEENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT
>Mature_257_residues
MEIQSQPTNLTKEDVIKLIAEFQQNQCGEAQERLVDHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDHLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEQTERRLVLKDIFNVLNETEKSVIHYIFEENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily

Homologues:

Organism=Escherichia coli, GI1788231, Length=196, Percent_Identity=30.1020408163265, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): RPSB_BACAN (Q9K5J6)

Other databases:

- EMBL:   AJ272497
- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_843493.1
- RefSeq:   YP_017620.1
- RefSeq:   YP_027202.1
- ProteinModelPortal:   Q9K5J6
- SMR:   Q9K5J6
- EnsemblBacteria:   EBBACT00000009251
- EnsemblBacteria:   EBBACT00000017740
- EnsemblBacteria:   EBBACT00000023287
- GeneID:   1088960
- GeneID:   2814654
- GeneID:   2850205
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_0992
- KEGG:   bar:GBAA_0992
- KEGG:   bat:BAS0928
- TIGR:   BA_0992
- TIGR:   GBAA_0992
- GeneTree:   EBGT00070000031820
- HOGENOM:   HBG745096
- OMA:   YSIAYRY
- ProtClustDB:   PRK08583
- BioCyc:   BANT260799:BAS0928-MONOMER
- BioCyc:   BANT261594:GBAA0992-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02980
- TIGRFAMs:   TIGR02937
- TIGRFAMs:   TIGR02941

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 29302; Mature: 29302

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629