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The map label for this gene is glpF [H]

Identifier: 227816119

GI number: 227816119

Start: 3223459

End: 3224274

Strand: Reverse

Name: glpF [H]

Synonym: BAMEG_3545

Alternate gene names: 227816119

Gene position: 3224274-3223459 (Counterclockwise)

Preceding gene: 227816120

Following gene: 227816118

Centisome position: 61.65

GC content: 40.44

Gene sequence:

>816_bases
ATGTCAGCATTTTTAGGAGAGTTAATAGGGACTGCGTTGTTAATCGTACTTGGTGGCGGCGTTTGTGCTGGTGTAAGTTT
AAAGAAGTCGTTTGCGAAAGATTCTGGTTGGATTGTTATTACAATGGGCTGGGGCTTAGCGGTAGCGGTTGCAGCGTACG
TGGTTGGATCAATTAGTGGGGCACATTTGAATCCAGCTTTGACGATAGGATTAGCATTTAAGGGAGCGTTCCCATGGGGT
GACGTACCTGGTTATATCGCAGCACAAATGATTGGGGCAATTATCGGGGCAGTTATCGTATATTTACATTACTTACCACA
CTGGAAAGAAACAGAAGATCCAGGAACAAAGTTAGGTGTATTTGCAACAGGTCCAGCAATTCCGAACACATTTGCAAACC
TTTTAAGTGAAATGATTGGGACATTCGTTTTAGTATTTGGTATATTAGCAATTGGTGCAAATAAATTTGCAGATGGATTA
AATCCATTTATCGTAGGTTTCTTAATTGTAAGTATTGGTTTATCATTAGGTGGAACGACAGGATACGCAATCAACCCAGC
ACGTGATTTAGGTCCGCGTATCGCGCACTTCTTCCTTCCGATTGCAGGAAAAGGCGGTTCAAACTGGAAGTATGCATGGA
TTCCAGTAGTAGGTCCGATTTTAGGTGGATCACTTGCAGGATTATTCCATCAAGTTGTATTTGAAGGAAAACAAAATGCA
GCACTTATTTATGTGATTATTGCAACTGTGATTGTATTAGCAATCTCTTATATGACAAGCAAAAAAGTGGAAGCAATACA
AATAGTAGAAAAGTAG

Upstream 100 bases:

>100_bases
GTGCAAAGCTGTGGGTCTTTTTCTTTTGACAAAAACGAATGGCCATTCATTTTACCTCTATCACATTTGAAAGCGTTTAA
ATTGATTGTGGAGGGATACT

Downstream 100 bases:

>100_bases
CATAAGGGGGAAACCATTATGAAAAAATATATTCTTTCATTAGACCAAGGAACAACTAGCTCACGCGCAATTCTTTTCAA
TAAAAAAGGTGAAATTGTTC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVVGSISGAHLNPALTIGLAFKGAFPWG
DVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGL
NPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNA
ALIYVIIATVIVLAISYMTSKKVEAIQIVEK

Sequences:

>Translated_271_residues
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVVGSISGAHLNPALTIGLAFKGAFPWG
DVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGL
NPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNA
ALIYVIIATVIVLAISYMTSKKVEAIQIVEK
>Mature_270_residues
SAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVVGSISGAHLNPALTIGLAFKGAFPWGD
VPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLN
PFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNAA
LIYVIIATVIVLAISYMTSKKVEAIQIVEK

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=242, Percent_Identity=38.0165289256198, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI157266307, Length=250, Percent_Identity=36.8, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4502187, Length=249, Percent_Identity=35.3413654618474, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI4755125, Length=206, Percent_Identity=34.9514563106796, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4502181, Length=206, Percent_Identity=34.9514563106796, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI37694062, Length=245, Percent_Identity=31.0204081632653, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310133356, Length=200, Percent_Identity=31, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310114181, Length=200, Percent_Identity=31, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790362, Length=259, Percent_Identity=34.3629343629344, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787101, Length=189, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994009, Length=229, Percent_Identity=33.6244541484716, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17544068, Length=246, Percent_Identity=34.9593495934959, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71992966, Length=234, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17533613, Length=240, Percent_Identity=30.4166666666667, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32564052, Length=240, Percent_Identity=30.4166666666667, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=259, Percent_Identity=30.8880308880309, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531431, Length=259, Percent_Identity=30.8880308880309, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=236, Percent_Identity=28.3898305084746, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=224, Percent_Identity=29.4642857142857, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=195, Percent_Identity=28.7179487179487, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=36.4077669902913, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322985, Length=291, Percent_Identity=27.1477663230241, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652747, Length=239, Percent_Identity=32.6359832635983, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45551084, Length=239, Percent_Identity=32.6359832635983, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24762342, Length=252, Percent_Identity=28.1746031746032, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24762344, Length=243, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762346, Length=243, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762348, Length=243, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130305, Length=243, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922828, Length=192, Percent_Identity=31.25, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28140; Mature: 28008

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVVGSISG
CCHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTIGLAFKGAFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGV
CCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLSLGGTT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNA
CCEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
ALIYVIIATVIVLAISYMTSKKVEAIQIVEK
CHHHHHHHHHHHHHHHHHHHCCHHHHHEECC
>Mature Secondary Structure 
SAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVVGSISG
CHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTIGLAFKGAFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGV
CCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLSLGGTT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNA
CCEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
ALIYVIIATVIVLAISYMTSKKVEAIQIVEK
CHHHHHHHHHHHHHHHHHHHCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]