Click here to switch to the map view.

The map label for this gene is asnO1 [H]

Identifier: 227815999

GI number: 227815999

Start: 3110919

End: 3112766

Strand: Reverse

Name: asnO1 [H]

Synonym: BAMEG_3425

Alternate gene names: 227815999

Gene position: 3112766-3110919 (Counterclockwise)

Preceding gene: 227816001

Following gene: 227815998

Centisome position: 59.52

GC content: 38.15

Gene sequence:

>1848_bases
ATGTGCGGGATTACAGGATGGGTGGATTATAAACGCTCATTAGAAGGAGAACGGGACGTCGTTACGAAGATGGCTGAGAC
GTTAGCGAAGCGTGGTCCAGATGATAATAAAGTTTGGATTAAAGGCAATGTCGCATTTGGGCATAAACGGTTAATCGTTG
TTGATCCTGAAGGTGGTAAACAGCCGATGACTTGTTTAAAGGATGAAGCAAATTATGCAATTTGCTATAACGGCGAACTG
TATAACACGGAAGACATTCGAAAGGAACTATTAAGAAGAGGATATACGTTCAAAGGTCACTCTGATACGGAAGTATTGTT
AGCTTCTTATATCGAATGGAAAGAAGAATGTGTCGATCATTTAAACGGTATATATGCGTTTGCTGTATGGGATGAACAGA
AAGAACAAGTGTTTATTGCGCGCGATCGATTAGGGGTAAAACCACTATTTTATAAATATGACAGTGGGCGATTACTATTT
GGCTCAGAGTTAAAAGCGATATTGGCGCATCCAGATGTGAAGGCGGAAGTGACGTTAGAAGGATTATCTGAAATATTTGG
CCTTGGACCATCTAGAACGCCTGGTCACGGTATTTATACTGGCATAAAAGAATTACGTCCAGGTCATGCGATGACATTTT
CGAAGAACGGTTTATGTATATGGAGATATTGGAATGTAGAAAGTAAAAAGCATGAAGATTCTTTTGAAGAAACAGTAGAA
AAAACACGCTTTTTATTACAAGATGCGATTACAAGGCAGCTCGTTTCTGATGTACCATTATGCACTTTTTTATCAGGCGG
TGTAGATTCAAGCGCTATAACAGCAATTGCTGCGAAAGAATACGAAAAATCGGGAAAAGGTCAATTACACACGTATTCGG
TTGATTACGAAGATAATGACAAATACTTTAAAGCAAATGCGTTTCAGCCAAATTCAGATGCACCATTTATTCATTTGATG
ACTGAGACGTTTCAAACGATCCATCATCGTTGCGTCATTTCAAATGAACAATTAGCGCAGTATTTAACAGAAGCAGTACT
CGTTCGTGATTTGCCTGGTATGGCAGATATTGATTCGTCATTATTATGGTTTTGTCGTGAAATTAAACAAGATTTTGTCG
TTGGTTTATCTGGGGAATGTGCAGATGAAATATTTGGTGGTTATCCATGGTTTTATAGAGAAGATGATTTACAATCGAGT
GCATTTCCGTGGATGCGTTCTACAGAAGCGCGTGAACAATTGCTAAAGAAAGAATGGAGAAATAAATTAAATTTACAACA
ATATGTACAGCAGCGCTATGAAGAATCCATTCAAGAAGTTCCTATTTTAGATGGGGAAAGTCCGCTAGAAGCAAAAAGAC
GCCAATTATTTTATTTAAATATGGTATGGTTTATGACAACATTATTAGACAGAAAAGACCGCATGAGTATGGGGGCAAGC
TTAGAAGTGCGCGTTCCATTTGCAGATCACCGACTTGTCGAATATGCGTGGAATATTCCTTGGGAAATGAAAATGTATAA
AAACCGCGAAAAAGGTCTATTGCGTAAAGCGTTAGAAGGGTTACTTCCAAATGACATCTTATATAGAAAGAAGAGCCCGT
ATCCAAAAACACATAATCCGCACTATACGAAAGCAGTAACAGTATGGATACAAGATTTATTAACGGATAAAGGCTCAATT
TTACACGAGCTATTTGATAAAGAACAGCTGAGTGGATTAATTGAGTCAGGCGGCAGTGCATTCCAAACGCCATGGTATGG
TCAATTAATGACTGGGCCACAGCTTTTAGCTCATTTAGCACAAATTCATGTTTGGTTTAAAGAATATGGAGTAAATATTA
AAGAATAG

Upstream 100 bases:

>100_bases
ATAGTAGGTTATCGTCAAGTTTGTCATTTTTTTGATCCGCACTTTTAGAGTGGGGTTACTGTACGTTCAAGAAAAGAAAT
AAGATGGAAGGATGATTTAT

Downstream 100 bases:

>100_bases
GAGAAAAGCGACTTACTTTATAAGTAAGTCGCTTTTTATTAAATAAATTTTCATATAAAATTCAAAATCATATTAGAAAA
AATACTTATTTAGAATAATT

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGEL
YNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLF
GSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYTGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLM
TETFQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSS
AFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSI
LHELFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Sequences:

>Translated_615_residues
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGEL
YNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLF
GSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYTGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLM
TETFQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSS
AFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSI
LHELFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
>Mature_615_residues
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGEL
YNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLF
GSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYTGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLM
TETFQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSS
AFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSI
LHELFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=570, Percent_Identity=25.9649122807018, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI168229252, Length=570, Percent_Identity=25.9649122807018, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI168229250, Length=570, Percent_Identity=25.9649122807018, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI296010848, Length=540, Percent_Identity=25.7407407407407, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI296010852, Length=470, Percent_Identity=24.0425531914894, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI296010850, Length=470, Percent_Identity=24.0425531914894, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786889, Length=557, Percent_Identity=26.032315978456, Blast_Score=159, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17560178, Length=611, Percent_Identity=25.531914893617, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71993933, Length=583, Percent_Identity=25.3859348198971, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=583, Percent_Identity=25.0428816466552, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=309, Percent_Identity=27.5080906148867, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321563, Length=569, Percent_Identity=24.9560632688928, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=570, Percent_Identity=24.7368421052632, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45553209, Length=537, Percent_Identity=25.3258845437616, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70940; Mature: 70940

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCC
QPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDH
CCHHHHHCCCCEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLE
CCCEEEEEEECCCCCEEEEECCCCCCCEEEEEECCCCEEECCHHHHHHCCCCCCEEEEHH
GLSEIFGLGPSRTPGHGIYTGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHH
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDND
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
KYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSS
CEEEECCCCCCCCCCEEEHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWR
HHHHHHHHHHHHEECCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
NKLNLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
HCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCE
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNP
EEEECCCCCCHHHHHHHCCCEEHHHHHCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
HYTKAVTVWIQDLLTDKGSILHELFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLA
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QIHVWFKEYGVNIKE
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCC
QPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDH
CCHHHHHCCCCEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLE
CCCEEEEEEECCCCCEEEEECCCCCCCEEEEEECCCCEEECCHHHHHHCCCCCCEEEEHH
GLSEIFGLGPSRTPGHGIYTGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHH
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDND
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
KYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSS
CEEEECCCCCCCCCCEEEHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWR
HHHHHHHHHHHHEECCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
NKLNLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
HCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCE
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNP
EEEECCCCCCHHHHHHHCCCEEHHHHHCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
HYTKAVTVWIQDLLTDKGSILHELFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLA
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QIHVWFKEYGVNIKE
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]