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The map label for this gene is dacB [H]

Identifier: 227815884

GI number: 227815884

Start: 3003044

End: 3004330

Strand: Reverse

Name: dacB [H]

Synonym: BAMEG_3309

Alternate gene names: 227815884

Gene position: 3004330-3003044 (Counterclockwise)

Preceding gene: 227815885

Following gene: 227815883

Centisome position: 57.44

GC content: 36.05

Gene sequence:

>1287_bases
ATGGTAAATTTTAAGAAAATTTCGGCTTTTATTACCGTTATTGGTTTATTTTTTTTGACACCTATGACGTTACGTGCTGA
GACGAATATTGGGGTGAACCCAGAACAAGTGGCACCACCACCTAAGGAAGGGCCAAATGTTTTTAGTCAGTTTGCAACTA
CAATTGATGCGAAAACTGGAGATATTTTGTATGATAAAAACGCACATCACCGTGCATTTCCGGCTAGTATGACGAAAGTG
TTAACGGCGATTTTACTTATGGAACATACGAAGCCAGAGGATCAATTTACATTTTCACAATTAGCATTAGATCAAGAGAA
GAGTAATTATCAAATTGAGTTTCAACCTGGTGAGACGATTAATAGAAATACTGCACTTATGATTTTAATGGTGCTTAGTG
CAAATGATGTGTCGTATGCGATTGCGGAGCGTATTGGCGGAAGTGTTGAGAATTTCGCTAATATGATGAATGAGAGAGCG
AAGCAATTAGGCGCTACCGACAGTCATTTTGTAACACCAAACGGATTGCATGATCCGAATCATTATACTACGCCATATGA
TATGGCTATGATTACGAGAGGTGTGCAAAAGTATCCTGAAATTTTACAAGCGATGAATACGAAAAGAACAACAGTTACGA
CATCTAGGCAGACGGTGTCTATTTTTAATAAATCTACTTATTTTGAAAATCCATTTAGTATTGGTGGTAAGACAGGTTTT
ACAAATGAAGCGCGCAATACACTCGTTTTATTAAATGAGAAGGATGGAAATCGTATTATAAACGTAGTAATGGCTTCTCA
AAGACCTGAAATTTATGAAGATTTAAAGCAGATGGCGGACTATTCTTTCGGTCAATTTGTGAAGCAAACTGTACTGGATA
AACATAGTTGGCATCAAAAGACAACGTATTTAAATAAAGATATTGATAGTGAGCTTGAAAAAAGTGCAGAACTAATGCTT
AAGAAAGATGAAGTGAAAAATGTGAAGACGACGTTCCGGGCGGCTTCATTAGATAAAGATTCTTTATATCATAAAGGAAT
TCATCGTGGTGAAGTAGTTGGTGCAGTTGATATTACGAAAAATAATCAAACAATTGCGACGGTAAATGTTCTTTCTAAAG
AAGACGTTACTTTTGCGATGCCTAAAAAAGATACGATCGAGCCTGAAGTAAACGAATCAAACGTGAAAATAATAAGCATT
GGAATAGGTGCTGCCATATTATTTGGAGCCATTTTATTTGTAGTGCTACGCCGAAATACAAAAGGAATGAAATCAGAAGA
AAAATAA

Upstream 100 bases:

>100_bases
GTAAAAAACAGTGAATGTTCTTCCAATTTAATACAAATTACATATAGATTGGTAGATGCAGTTTTTAAGCTGTGAGAGTA
TAGTGAAAAGGGAGAGTCAT

Downstream 100 bases:

>100_bases
ATTACTAGTTTAAAAGGACTGTGAAGAGACTTCTTCACAGTCCTTTTTTTATATTAAAAATAACTAATAATTGTAAATTT
TCTTATTGCATTTCTAATGT

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVNFKKISAFITVIGLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAELML
KKDEVKNVKTTFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISI
GIGAAILFGAILFVVLRRNTKGMKSEEK

Sequences:

>Translated_428_residues
MVNFKKISAFITVIGLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAELML
KKDEVKNVKTTFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISI
GIGAAILFGAILFVVLRRNTKGMKSEEK
>Mature_428_residues
MVNFKKISAFITVIGLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERA
KQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLNKDIDSELEKSAELML
KKDEVKNVKTTFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISI
GIGAAILFGAILFVVLRRNTKGMKSEEK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=375, Percent_Identity=24.2666666666667, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082022, Length=356, Percent_Identity=24.438202247191, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786851, Length=336, Percent_Identity=24.1071428571429, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082054, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47948; Mature: 47948

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNFKKISAFITVIGLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTG
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCC
DILYDKNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETI
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
NRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERAKQLGATDSHFVTPNGLHDPN
CCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCC
HYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHCCCCCCCCCCCCCCCCCC
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQK
CCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
TTYLNKDIDSELEKSAELMLKKDEVKNVKTTFRAASLDKDSLYHKGIHRGEVVGAVDITK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEC
NNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISIGIGAAILFGAILFVVLRRNT
CCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
KGMKSEEK
CCCCCCCC
>Mature Secondary Structure
MVNFKKISAFITVIGLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTG
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCC
DILYDKNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETI
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
NRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERAKQLGATDSHFVTPNGLHDPN
CCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCC
HYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSTYFENPFSIGGKTGF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHCCCCCCCCCCCCCCCCCC
TNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQK
CCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
TTYLNKDIDSELEKSAELMLKKDEVKNVKTTFRAASLDKDSLYHKGIHRGEVVGAVDITK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEC
NNQTIATVNVLSKEDVTFAMPKKDTIEPEVNESNVKIISIGIGAAILFGAILFVVLRRNT
CCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
KGMKSEEK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]