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The map label for this gene is potB [H]

Identifier: 227815873

GI number: 227815873

Start: 2992762

End: 2993610

Strand: Reverse

Name: potB [H]

Synonym: BAMEG_3298

Alternate gene names: 227815873

Gene position: 2993610-2992762 (Counterclockwise)

Preceding gene: 227815874

Following gene: 227815872

Centisome position: 57.24

GC content: 36.75

Gene sequence:

>849_bases
TTGAAAAAAGGGAAATTACTCGCACTACCTACAGTCGCATGGCTGTTAATCTTCTTCCTAATTCCCTTTCTGTTTGTATT
GGCATTTGCCTTCATGCAGCGTGGGGCGTATGGAACAGTGGAAATGCAATTTACATTAGAAAACATAGCACGAGTATTTG
ACCCATTATATATGGGGACGTTATGGGAAACAGTGAAAATTGCGGTTATTACTACAGTAATATGTTTACTGATCGGTTAT
CCATTTGCATATACAATTACAATTGTTGATCGCAAATATCGTTCCATTCTTTTATTATTGGCAACGATCCCGTTTTGGAT
TAACTTCCTTGTTCGTTCATACGCATGGATTGTTATTTTACGTTCACAAGGTCTTGTGAACACGTTTTTATTAAAGCTCG
GTATTATTAGTGAACCTCTAAATTTACTATATAATACACCTTCTGTAATACTCGGAATGGTATATTCTTTATTACCATTT
ATGATTTTACCAGTGTATGCAGCGATTGAGCAGCTTGATAAGCGTAAGCTAGAAGCAGCTTATGATTTAGGGGCAACACC
AGTAAAGGCATTTTGGAATGTTACAGTACCAATGACGATGTCAGGGATTGCTACTGGTTCTATTTTAGTGTTTGTTTCTT
CTATCGGAATGTTTGTTGTATCAGACGTTATGGGTGGATCGAAAGTAGCATTAATCGGAAACGTAATTCAAAATCAATTC
TTAGGAGCGCGTGATTGGCCGTTTGGATCTGCGTTATCTATGATTGTTGTTCTATTCTCTGTTCTGTTAATTTACTTATA
TTATCGTGCAACGAAAGTATATAAATATGATGGGAACGGAGGGGAATAG

Upstream 100 bases:

>100_bases
AATTACTAATGGCATATCAAACTGCTGAAGAAGCAGCGCAGTGGAGCATTGGAGATAATGTATACGTAGGCTGGGAGCAA
GAGGACGAGGTGACCTTAAA

Downstream 100 bases:

>100_bases
GCAAAGATGAAGAAGTTTTTAGTTTCTTATTCTTGGTTAATTTTATTGTTCTTGTATTTTCCAATGATGGTTTTAATGGT
GTATTCCTTCAATGATTCTC

Product: spermidine/putrescine ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKKGKLLALPTVAWLLIFFLIPFLFVLAFAFMQRGAYGTVEMQFTLENIARVFDPLYMGTLWETVKIAVITTVICLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTFLLKLGIISEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQLDKRKLEAAYDLGATPVKAFWNVTVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSMIVVLFSVLLIYLYYRATKVYKYDGNGGE

Sequences:

>Translated_282_residues
MKKGKLLALPTVAWLLIFFLIPFLFVLAFAFMQRGAYGTVEMQFTLENIARVFDPLYMGTLWETVKIAVITTVICLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTFLLKLGIISEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQLDKRKLEAAYDLGATPVKAFWNVTVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSMIVVLFSVLLIYLYYRATKVYKYDGNGGE
>Mature_282_residues
MKKGKLLALPTVAWLLIFFLIPFLFVLAFAFMQRGAYGTVEMQFTLENIARVFDPLYMGTLWETVKIAVITTVICLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTFLLKLGIISEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQLDKRKLEAAYDLGATPVKAFWNVTVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSMIVVLFSVLLIYLYYRATKVYKYDGNGGE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=262, Percent_Identity=44.2748091603053, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1787080, Length=247, Percent_Identity=40.4858299595142, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=32.0987654320988, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31556; Mature: 31556

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGKLLALPTVAWLLIFFLIPFLFVLAFAFMQRGAYGTVEMQFTLENIARVFDPLYMGT
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHH
LWETVKIAVITTVICLLIGYPFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVIL
HHHHHHHHHHHHHHHHHHCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
RSQGLVNTFLLKLGIISEPLNLLYNTPSVILGMVYSLLPFMILPVYAAIEQLDKRKLEAA
ECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDLGATPVKAFWNVTVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
HCCCCCCHHHHHEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGARDWPFGSALSMIVVLFSVLLIYLYYRATKVYKYDGNGGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MKKGKLLALPTVAWLLIFFLIPFLFVLAFAFMQRGAYGTVEMQFTLENIARVFDPLYMGT
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHH
LWETVKIAVITTVICLLIGYPFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVIL
HHHHHHHHHHHHHHHHHHCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
RSQGLVNTFLLKLGIISEPLNLLYNTPSVILGMVYSLLPFMILPVYAAIEQLDKRKLEAA
ECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDLGATPVKAFWNVTVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
HCCCCCCHHHHHEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGARDWPFGSALSMIVVLFSVLLIYLYYRATKVYKYDGNGGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]