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The map label for this gene is potD [H]

Identifier: 227815871

GI number: 227815871

Start: 2990879

End: 2991916

Strand: Reverse

Name: potD [H]

Synonym: BAMEG_3296

Alternate gene names: 227815871

Gene position: 2991916-2990879 (Counterclockwise)

Preceding gene: 227815872

Following gene: 227815870

Centisome position: 57.21

GC content: 34.39

Gene sequence:

>1038_bases
ATGAAATTAACGAAGAGATTAGCTGGCGCAGCAATTAGCTTTAGCCTTGTTGCTGGTTGTGGTGAAAAGAAAGAAGAGTT
AAACATTTATAGCTGGGCTGACAATTTTGATGAGCAAGTGCTAAGAGATTTTGAAAAGAAATATAACGTGAAAATTAACT
ATGATAAGTATGCAAGTAATGAAGAAATGCTTGCGAAATTACAAGCTGGTGGCGCGAAGTATGATTTAATTCAGCCGTCT
GACTACATGGTTAAAACAATGGCAAAAATGGATTTATTAGCGCCGTTAGATAAAAAGAATATCCCAAATATCGAAAACAT
GGTTTCTAATTTCAAAACACCTGCGTTTGATCCAGAGAATAAATATTCTTTAGTATACACTTGGGGTGTAACTGGGATTG
CATACAATAAAAAGTATGTGAAAGAAGCACCTACAAGTTGGACTGACTTATGGAATGAGAAATATAAAGGGCATGTAACT
TTATTAAATGATTCTCGTGAAGTATTAGGTATGGGTCTTAAGAAAAACGGCTTCTCAAACAGTACGAAAGATGATGCACA
GTTAAAGACAGCAGCAACTGATTTACAGAAGCTACTTCCAAACTTATTAGCATTTGATACAGATAATATTAAACAAAAGT
TCATTACAGAAGATGCTTGGATCGGAACAGTTTGGTCTGGAGATGCAGCATTTATCGCAAAAGATAATAAAGATGTAGAG
TACGTTGTACCAAAAGAAGGCGGCACAATTTGGGCTGATACGTTAGCAATTCCAAAAGGTGCGAAAAATAAAGAGCTTGC
AGAGAAGTTTATGAACTACTTATTAGATGAAAAAGTAAGTGTGAAAAACTACGAGTCAATTGGATACAGTAATCCAAATG
AAAAAGCGCATCCTCTTCATAGTAAAGAATATCGTGAAAACCATATGATTTTCTTAACGAAAGAAGAATTAGATCGTACA
GAATGGCTTGTTGATGTAGATGATAAGTTAAAAGATTATGATCGTTACTGGACAGAGTTAAAAACAAAAGGTAAATAA

Upstream 100 bases:

>100_bases
AAATCTTCCGTAACAAAGGTGCAGATGGTGAAGAAAACTCTGGAGGACACCTTCCTTTATAATACGAACGATATAGAAAG
TACGAGGGGGTAATAAACCG

Downstream 100 bases:

>100_bases
GTAAGGAAATGCGGTTTCTAAAGTAGAAATCGCATTTTTTCATAGTAGGAGGAAATCGTTTGAAGAAGAAGTTGCATCAA
TATGAGGTAATGTGTATCGG

Product: spermidine/putrescine ABC transporter, spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKLTKRLAGAAISFSLVAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMLAKLQAGGAKYDLIQPS
DYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKEAPTSWTDLWNEKYKGHVT
LLNDSREVLGMGLKKNGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDNKDVE
YVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKAHPLHSKEYRENHMIFLTKEELDRT
EWLVDVDDKLKDYDRYWTELKTKGK

Sequences:

>Translated_345_residues
MKLTKRLAGAAISFSLVAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMLAKLQAGGAKYDLIQPS
DYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKEAPTSWTDLWNEKYKGHVT
LLNDSREVLGMGLKKNGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDNKDVE
YVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKAHPLHSKEYRENHMIFLTKEELDRT
EWLVDVDDKLKDYDRYWTELKTKGK
>Mature_345_residues
MKLTKRLAGAAISFSLVAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMLAKLQAGGAKYDLIQPS
DYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKEAPTSWTDLWNEKYKGHVT
LLNDSREVLGMGLKKNGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDNKDVE
YVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKAHPLHSKEYRENHMIFLTKEELDRT
EWLVDVDDKLKDYDRYWTELKTKGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=328, Percent_Identity=36.5853658536585, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1787078, Length=361, Percent_Identity=32.6869806094183, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1787711, Length=257, Percent_Identity=26.0700389105058, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39520; Mature: 39520

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKRLAGAAISFSLVAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASN
CCHHHHHHHHHHHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHCCEEEEEHHHCCC
EEMLAKLQAGGAKYDLIQPSDYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAFDPEN
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCC
KYSLVYTWGVTGIAYNKKYVKEAPTSWTDLWNEKYKGHVTLLNDSREVLGMGLKKNGFSN
CEEEEEEECCCEEEECHHHHHHCCCHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCC
STKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDNKDVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCEEEEEECCCCEE
YVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKAHPLH
EEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCC
SKEYRENHMIFLTKEELDRTEWLVDVDDKLKDYDRYWTELKTKGK
CHHHCCCCEEEEEHHHCCCHHEEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLTKRLAGAAISFSLVAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASN
CCHHHHHHHHHHHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHCCEEEEEHHHCCC
EEMLAKLQAGGAKYDLIQPSDYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAFDPEN
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCC
KYSLVYTWGVTGIAYNKKYVKEAPTSWTDLWNEKYKGHVTLLNDSREVLGMGLKKNGFSN
CEEEEEEECCCEEEECHHHHHHCCCHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCC
STKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDNKDVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCEEEEEECCCCEE
YVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKAHPLH
EEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCC
SKEYRENHMIFLTKEELDRTEWLVDVDDKLKDYDRYWTELKTKGK
CHHHCCCCEEEEEHHHCCCHHEEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]