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The map label for this gene is kinB1 [H]

Identifier: 227815814

GI number: 227815814

Start: 2940509

End: 2941717

Strand: Direct

Name: kinB1 [H]

Synonym: BAMEG_3238

Alternate gene names: 227815814

Gene position: 2940509-2941717 (Clockwise)

Preceding gene: 227815809

Following gene: 227815819

Centisome position: 56.22

GC content: 33.17

Gene sequence:

>1209_bases
ATGCTCGTATACCATTTATTTTGGAATCAAAAAGGGAAACGTTCTCCTAAATTAAATTCAGCAATATTTATTGTATTATG
TTGCCTCGTTACTATACTATGTATAACTTTCGCAGCAAAAACAATTTACGGATTTCAATTTGATATGCGTCATATCGTAT
TAATTGTCGGTACATTAACCGGAGGTCCTATTGCTGGTGGATCTATATTAGTTGTATTAAATATATACCGCTTCTTATTA
GGAGGAATAGGTGTTTTCCCATCACTTATCGGTTCTATCCTTCTATTTATTGTCCTATTATTTACATACAAATTTTTCAA
TCGAACTTCTAATCGTATAAAAATAACACTTGCTATTATCTACAGTCTCATATACGGATTTAGTTGGATACCTTTCTTCC
TTTCCAAGGTTACAAACAAGGCTGATTATATACCACATATTATTGTGTATGAGTTATGTACGATACTGGGTACGATTCTT
ATTTTATATTTACTACATATATTACAAACTCAAGTTCGACTCCAAAACGAACTTATGAATGCTGAAAAATTTCATTTAAT
TGGTGAAATGGCAGCGTCTATCTCTCATGAAATTCGCAATCCGTTAACTTCAACGAAAGGATTTTTGCAACTTTTACAAT
CAGATACATGCACTGAGCAGGAGCGAAAATTATATATCGACATAGCCATCAACGGAATCGAACAAGCAAACCACGTTCTT
ACAGATTATTTAACCTTTGCAAAACCAAGTATTGAAAAAGAACAAAGATTACAGTTAGAAGAAGAGCTGCTGCACGCATT
ATCGTTAATTACACCGCTTGCTAATTTAACAAATGTGCGTACACATTATATAAAGCAAAGCACTTCTTTTTTTATCGCGG
GAGAAAAACAAAAACTAAATCAATGCTTGTTAAATATTTTAAAAAACTGCATTGAGGCTATGCCAAAAGGCGGTGATCTT
TATTTCACATTAGTTCCTGATCATAAGCATATTCAGTTATATATAAAAGATACAGGAGTTGGTATGGATCAAGAACAAGT
AAAACGCCTTGGATCCCCTTTCTACTCAACAAAAGAAAAAGGTGCTGGCCTTGGAATGATGGTCGTATTCAGTGTTGTTC
AAGCTATGAATGGAAAAATTGATATTATAAGCGAAAAAGGTACAGGTACAACCTTCTTATTAACTTTTCCACTCATACAA
AAAACGTGA

Upstream 100 bases:

>100_bases
TTTTATAGTATGATTAATTTAATGTAAGAAGAGAGGAGGCGTCCCACTGAAATCCTTTATTATTTATCTCTTTTTAAATA
TATTATTCATCCTTGTTACA

Downstream 100 bases:

>100_bases
TGAAATACTCATCACGTTTTTTCTGTTCCTTCAACTAATTCAGCAAAAGCTTTCACTTTGCCATGTGGTTTTTGATTTTC
TTTACGTGCTTTCCAAGCTC

Product: sporulation kinase B

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLTGGPIAGGSILVVLNIYRFLL
GGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAIIYSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTIL
ILYLLHILQTQVRLQNELMNAEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLNQCLLNILKNCIEAMPKGGDL
YFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEKGAGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQ
KT

Sequences:

>Translated_402_residues
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLTGGPIAGGSILVVLNIYRFLL
GGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAIIYSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTIL
ILYLLHILQTQVRLQNELMNAEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLNQCLLNILKNCIEAMPKGGDL
YFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEKGAGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQ
KT
>Mature_402_residues
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLTGGPIAGGSILVVLNIYRFLL
GGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAIIYSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTIL
ILYLLHILQTQVRLQNELMNAEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLNQCLLNILKNCIEAMPKGGDL
YFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEKGAGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQ
KT

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=254, Percent_Identity=28.3464566929134, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788549, Length=227, Percent_Identity=29.0748898678414, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=28.3842794759825, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=25.1063829787234, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786783, Length=219, Percent_Identity=26.027397260274, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790861, Length=292, Percent_Identity=23.2876712328767, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI48994928, Length=221, Percent_Identity=22.6244343891403, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=22.4770642201835, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=27.8481012658228, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45323; Mature: 45323

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLT
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEECC
GGPIAGGSILVVLNIYRFLLGGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
YSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTILILYLLHILQTQVRLQNELMN
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHH
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
QCLLNILKNCIEAMPKGGDLYFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEK
HHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCHHC
GAGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQKT
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHCC
>Mature Secondary Structure
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLT
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEECC
GGPIAGGSILVVLNIYRFLLGGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
YSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTILILYLLHILQTQVRLQNELMN
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHH
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
QCLLNILKNCIEAMPKGGDLYFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEK
HHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCHHC
GAGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQKT
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]