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The map label for this gene is queF

Identifier: 227815810

GI number: 227815810

Start: 2937786

End: 2938283

Strand: Reverse

Name: queF

Synonym: BAMEG_3233

Alternate gene names: 227815810

Gene position: 2938283-2937786 (Counterclockwise)

Preceding gene: 227815811

Following gene: 227815808

Centisome position: 56.18

GC content: 33.53

Gene sequence:

>498_bases
ATGGCAGGAAGATTAGATGAAGATTTAAAAGATGTAACATTATTAGGAAATCAAAATACAAAATATTTATTTGAATACAG
TCCAGAAATTTTAGAGGTATTTGATAACAATCATCCAAACCGTGATTATTTTGTTAAATTTAATTGTCCTGAATTTACAA
GCTTATGTCCGAAAACAGGCCAACCGGATTTTGCAACAATTTATATTAGCTACATTCCAGAACAAAGAATGGTAGAGAGT
AAATCTCTAAAGCTATATTTATTTAGTTTCCGCAATCACGGTGATTTTCACGAAGATTGCATGAACGTTATTATGAATGA
TTTAATTAAATTAATGGATCCACGTTACATTGAGGTATGGGGGAAATTCACACCACGTGGAGGGATTTCAATTGATCCTT
ACTGCAACTACGGTCGTCCAGGAACGAAATATGAACAAATGGCAGACTACCGCATGATGAACCATGATCTATATCCAGAA
ACAATTGACAACCGTTAA

Upstream 100 bases:

>100_bases
AGTTAACCGTAAAGAAATGAATAATGCAAAAGTATTGCCGCAGCTTCATGCGTTAGTATGGGGAAATAAACGCGGAGTAT
AATGAGAAGGGATGTTTAAA

Downstream 100 bases:

>100_bases
TATATAATAGAAAGAAAGGATCTGTATGTTATACAGATCCTTTCTTTCTATTATATTATGCATTTTGATTAATGACAGTA
TAGTTTTTATGACTAACAAC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MAGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQRMVES
KSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPE
TIDNR

Sequences:

>Translated_165_residues
MAGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQRMVES
KSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPE
TIDNR
>Mature_164_residues
AGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQRMVESK
SLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPET
IDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=113, Percent_Identity=33.6283185840708, Blast_Score=73, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_BACAA (C3P4F9)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002865856.1
- ProteinModelPortal:   C3P4F9
- SMR:   C3P4F9
- EnsemblBacteria:   EBBACT00000130279
- GeneID:   7850053
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_1430
- GeneTree:   EBGT00050000002144
- ProtClustDB:   PRK13258
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 19528; Mature: 19397

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTG
CCCCCCCCCHHEEEECCCCCEEEEEECHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCC
QPDFATIYISYIPEQRMVESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVW
CCCEEEEEEEECCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
GKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPETIDNR
ECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTG
CCCCCCCCHHEEEECCCCCEEEEEECHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCC
QPDFATIYISYIPEQRMVESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVW
CCCEEEEEEEECCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
GKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPETIDNR
ECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA