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The map label for this gene is nrdE [H]

Identifier: 227815801

GI number: 227815801

Start: 2933451

End: 2934182

Strand: Reverse

Name: nrdE [H]

Synonym: BAMEG_3224

Alternate gene names: 227815801

Gene position: 2934182-2933451 (Counterclockwise)

Preceding gene: 227815802

Following gene: 227815800

Centisome position: 56.1

GC content: 37.84

Gene sequence:

>732_bases
ATGCAGGACGGTTTTTATCAGCTTCATAAAGATAAAGAGGCATTAGAAGTCTTTATGGAAGAAGCTAGAGAGAATACCGT
TCATTTTAATAGCGTGGCAGAGCGAATGGAGTATATGAAAGAACATGATTACTATTACAACGTTCTGGACGAGTATAACT
TGGAGGAAGTAGAAGAGGTATATAACATCGCTTATGGTGAAAACTTCGAGTTCCAATCTTATATGGCAGCATCTAAGTTT
TACAAAGATTATGCGTTAAAAACAAATGATCAAAAACAATACTTAGAAAGCTATGAAGATCGTGTAGCAATTGTGTCATT
ATACTTAGGACGCGGTGATGTTGCCAAGGCAAAACAATTCGCAAGCATGATTGTCAAACAAAACTACCAACCAGCAACAC
CAACCTTTTTAAATGCGGGAAGAAGCAGAAGAGGAGAAATGGTGTCTTGTTTCTTGTTAGAGATGGATGATAGCTTAAAT
TCAATCGGCTTTAACATTAATACTGCAATGCAATTATCAAAAATCGGCGGAGGAGTAGCTTTAAACTTATCTAAGCTACG
CGCACGTGGTGAGCAAATTAAAGGTATCGACAACGCTGCAAGTGGTGTAGTACCTGTTATGAAGTTACTTGAAGATTCGT
TTTCATATGCGAATCAGCTTGGCCAACGAAAAGGTGCTGGCGCAGTATACTTGAACATTTTCCATTGGGATATTATTGAA
TTCCTGGATTAA

Upstream 100 bases:

>100_bases
TCAAAATTTGAGTTATCAGGAACAAATAACGATGTAGAATATTTTAAGGAAAGGGTGCGGGAGATTGCGACACATTGAAC
TGAATAATGAAATCACGCAA

Downstream 100 bases:

>100_bases
TTGGTAGTCCCTCTACACAGTAATGTGTATGAGAAAACCTTGTGAACCTAACCAATAGGGTGTCGCATTAGCGGCTAACG
GTATCAGTTGAATAAGGGGT

Product: ribonucleoside-diphosphate reductase, alpha subunit

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGENFEFQSYMAASKF
YKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLN
SIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
FLD

Sequences:

>Translated_243_residues
MQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGENFEFQSYMAASKF
YKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLN
SIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
FLD
>Mature_243_residues
MQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGENFEFQSYMAASKF
YKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLN
SIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
FLD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=183, Percent_Identity=32.2404371584699, Blast_Score=95, Evalue=4e-20,
Organism=Escherichia coli, GI1789030, Length=242, Percent_Identity=49.5867768595041, Blast_Score=241, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17555242, Length=182, Percent_Identity=31.3186813186813, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322123, Length=235, Percent_Identity=26.3829787234043, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320914, Length=183, Percent_Identity=29.5081967213115, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17352481, Length=203, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 27871; Mature: 27871

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEV
CCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHH
YNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQF
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEHEECCCCCHHHHHHH
ASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGGVA
HHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHCCCCHHHHHHHHHCCCCEE
LNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
FLD
HCC
>Mature Secondary Structure
MQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEV
CCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHH
YNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQF
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEHEECCCCCHHHHHHH
ASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGGVA
HHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHCCCCHHHHHHHHHCCCCEE
LNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
FLD
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]