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The map label for this gene is nrdF [H]

Identifier: 227815798

GI number: 227815798

Start: 2929970

End: 2930938

Strand: Reverse

Name: nrdF [H]

Synonym: BAMEG_3221

Alternate gene names: 227815798

Gene position: 2930938-2929970 (Counterclockwise)

Preceding gene: 227815799

Following gene: 227815797

Centisome position: 56.04

GC content: 35.81

Gene sequence:

>969_bases
ATGCGTGCGGTAAACTGGAACAAAAAAGAAGATGATTTTAGTTTAATGTTTTGGAAGCAAAACATCGCTCAGTTTTGGAC
AGAAGAAGAAATCGCGGTGTCTTCTGACAAAAATACTTGGGTGCAATTATCAAAAGAAGAGCAAATTGCTTATAAGCGTG
TATTAGGTGGTTTAACACTTTTAGATACGAAACAAGGCGGCGAAGGGATGCCACTTGTACTTGTTCATCTTGAAAATTTA
CAAGCAAAAAGCGTATTAGCTTTCATGGGTGCTATGGAAGAAGTACATGCGAAGAGTTATAGTCATATTTTCACAACGTT
AGCTACTGAAGAAGAAATCGATGAGATTTTTGACTGGGTAGATACTCATCCGTTACTTGAGAAAAAGGCTGGTATTATTA
CAAGTTACTATCGTCGTTTATTAAAACCTGAAGTGACGAAAAAAGAGTTATACATGGCAATGGTAGCAAGTGTGTTCTTA
GAAAGTTACTTATTCTATAGTGGATTCTTCTATCCACTTTACTTAGCTGGTCAAGGAAAACTTACGGCGAGTGGTGAGAT
TATTAACTTAATAATTCGTGATGAGTCAATTCACGGCGTATTCGTCGGTATTTTAGCACAACAAATCTTCGCAGAACTTT
CTGCAGAAGATCAACAAGAAGTGCAAAAAGAAACGCAAGAGTTATTAATGGAACTATATGAAATCGAAATGGCATACACA
GAAGAAATTTACACTTCTATCGGTCTTGTAGAAGATGTAAATCGCTTCGTTCGTTACAATGCGAATAAAGGACTTATGAA
CTTAGGACTTGAGCCGAAGTTTGAAGAAGAAGAAATTAACCCAATCGTTTTAAATGGTTTACGTACAGATACGAAAAACC
ATGATTTCTTCTCTGTAAAAGGAAATGGTTACGTAAAAGCAACGAACGTTGAAAAGTTATCTGATGATGACTTCGTGTTT
AATTTTTAA

Upstream 100 bases:

>100_bases
TGTTTAATATAAAAAGTGAGACATGATGAGCACGGTTTTCTGAACCCTGCTCATGCTCAGTTATGAATTTTTCATGTTTA
TTTAGAGAAGGGGCGATTGA

Downstream 100 bases:

>100_bases
TATATGATCATAAAGAAAAGCTGTCTTATTCATTGATAAGACAGCTTTTCTTTATGATTTTGTTTACATAACACTATTAT
TTTCATATAACGATTTCTGT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRAVNWNKKEDDFSLMFWKQNIAQFWTEEEIAVSSDKNTWVQLSKEEQIAYKRVLGGLTLLDTKQGGEGMPLVLVHLENL
QAKSVLAFMGAMEEVHAKSYSHIFTTLATEEEIDEIFDWVDTHPLLEKKAGIITSYYRRLLKPEVTKKELYMAMVASVFL
ESYLFYSGFFYPLYLAGQGKLTASGEIINLIIRDESIHGVFVGILAQQIFAELSAEDQQEVQKETQELLMELYEIEMAYT
EEIYTSIGLVEDVNRFVRYNANKGLMNLGLEPKFEEEEINPIVLNGLRTDTKNHDFFSVKGNGYVKATNVEKLSDDDFVF
NF

Sequences:

>Translated_322_residues
MRAVNWNKKEDDFSLMFWKQNIAQFWTEEEIAVSSDKNTWVQLSKEEQIAYKRVLGGLTLLDTKQGGEGMPLVLVHLENL
QAKSVLAFMGAMEEVHAKSYSHIFTTLATEEEIDEIFDWVDTHPLLEKKAGIITSYYRRLLKPEVTKKELYMAMVASVFL
ESYLFYSGFFYPLYLAGQGKLTASGEIINLIIRDESIHGVFVGILAQQIFAELSAEDQQEVQKETQELLMELYEIEMAYT
EEIYTSIGLVEDVNRFVRYNANKGLMNLGLEPKFEEEEINPIVLNGLRTDTKNHDFFSVKGNGYVKATNVEKLSDDDFVF
NF
>Mature_322_residues
MRAVNWNKKEDDFSLMFWKQNIAQFWTEEEIAVSSDKNTWVQLSKEEQIAYKRVLGGLTLLDTKQGGEGMPLVLVHLENL
QAKSVLAFMGAMEEVHAKSYSHIFTTLATEEEIDEIFDWVDTHPLLEKKAGIITSYYRRLLKPEVTKKELYMAMVASVFL
ESYLFYSGFFYPLYLAGQGKLTASGEIINLIIRDESIHGVFVGILAQQIFAELSAEDQQEVQKETQELLMELYEIEMAYT
EEIYTSIGLVEDVNRFVRYNANKGLMNLGLEPKFEEEEINPIVLNGLRTDTKNHDFFSVKGNGYVKATNVEKLSDDDFVF
NF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=323, Percent_Identity=41.1764705882353, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1788567, Length=306, Percent_Identity=25.4901960784314, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17551968, Length=299, Percent_Identity=22.742474916388, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 37021; Mature: 37021

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVNWNKKEDDFSLMFWKQNIAQFWTEEEIAVSSDKNTWVQLSKEEQIAYKRVLGGLTL
CCCCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHHHCCCEE
LDTKQGGEGMPLVLVHLENLQAKSVLAFMGAMEEVHAKSYSHIFTTLATEEEIDEIFDWV
EECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
DTHPLLEKKAGIITSYYRRLLKPEVTKKELYMAMVASVFLESYLFYSGFFYPLYLAGQGK
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
LTASGEIINLIIRDESIHGVFVGILAQQIFAELSAEDQQEVQKETQELLMELYEIEMAYT
EEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EEIYTSIGLVEDVNRFVRYNANKGLMNLGLEPKFEEEEINPIVLNGLRTDTKNHDFFSVK
HHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEEEECEECCCCCCCEEEEC
GNGYVKATNVEKLSDDDFVFNF
CCCEEEECCCCCCCCCCEEEEC
>Mature Secondary Structure
MRAVNWNKKEDDFSLMFWKQNIAQFWTEEEIAVSSDKNTWVQLSKEEQIAYKRVLGGLTL
CCCCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHHHCCCEE
LDTKQGGEGMPLVLVHLENLQAKSVLAFMGAMEEVHAKSYSHIFTTLATEEEIDEIFDWV
EECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
DTHPLLEKKAGIITSYYRRLLKPEVTKKELYMAMVASVFLESYLFYSGFFYPLYLAGQGK
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
LTASGEIINLIIRDESIHGVFVGILAQQIFAELSAEDQQEVQKETQELLMELYEIEMAYT
EEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EEIYTSIGLVEDVNRFVRYNANKGLMNLGLEPKFEEEEINPIVLNGLRTDTKNHDFFSVK
HHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEEEECEECCCCCCCEEEEC
GNGYVKATNVEKLSDDDFVFNF
CCCEEEECCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]