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The map label for this gene is ilvE1 [H]

Identifier: 227815756

GI number: 227815756

Start: 2899086

End: 2899982

Strand: Reverse

Name: ilvE1 [H]

Synonym: BAMEG_3179

Alternate gene names: 227815756

Gene position: 2899982-2899086 (Counterclockwise)

Preceding gene: 227815757

Following gene: 227815755

Centisome position: 55.45

GC content: 36.34

Gene sequence:

>897_bases
GTGAACGAGCAATGGATTTTCTTAAATGGAGAATTTGTTCCAAAAGACGAGGCAAAAGTATCAGTATATGATCATGGTTA
TTTATATGGCGATGGAGTATTTGAAGGAATTAGAGTTTATAGCGGTAATGTTTTCCGTTTGAGAGAGCATCTTGTCCGGT
TATATGAATCAGCAAAATCCATCATGTTAGAAATTCCATATTCTTTAGATGAAATAACGAATATTGTTGTTGAGACGATC
CGACAAAATAAATTATCTAATGGATACATTCGCCTAGTTGTATCGAGAGGAGCAGGAAATCTTGGGTTAGATCCTGATTC
TTGTACGAAACCGAATGTAGTAGTAATTGCAGAGCAACTATCTTTATTCCCGCAAGAGTATTATGAAAAAGGGATTCCAG
TTGTAACAGTTGCAACGCGCCGAAACCGTCCAGATGTATTGTCGCCTCAAGTAAAATCTTTAAATTACTTAAACAATATT
TTAGTTCGCATCGAAGCGAAATTGGCTGGAGTACAAGAAGCGCTTATGTTAAATGATCAAGGATACGTAGCTGAAGGCTC
AGGAGATAACGTGTTTATTGTAAAAGGAAATAAATTAATTACACCACCAAGTTCTGCTGGAGCGTTAGAAGGTATTACAC
GAAACGCTATTTTAGAAATCGGTGAAAAACTTGGATATGACGTTAGAGAAGAGTTATTTACAAGACATGATGTATATGTA
GCTGATGAAGTATTTTTAACAGGAACGGCTGCGGAAGTAATTGCTGTTACGACAGTAGATGGTAGAACGATCGGTTTAGG
GCAAACAGGTCCACATACGAATCGTTTATTAGAAGAATTCCGAAAGTTAGTTATAGAAGATGGAGAGAAAATTTACGAAG
AAAATAAAGTTGGATAA

Upstream 100 bases:

>100_bases
TAAAAATTATAAATTTTCTAAAAATTCACTTGATTTATTTAGAAAGGAGAGTAAACTAGGAAACAATAAAAAATTCATAG
AACATAGGAGGCGCTTTCCA

Downstream 100 bases:

>100_bases
CAACTTTTTATAACATAAGAAAGCGTTGAGAGGGAGGAGTAGTTGATTGTGAAGCCTCACAGAGAGTCGGTGGTTGCTGG
AAACCGATGGTTCACGTCGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKSIMLEIPYSLDEITNIVVETI
RQNKLSNGYIRLVVSRGAGNLGLDPDSCTKPNVVVIAEQLSLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNI
LVRIEAKLAGVQEALMLNDQGYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVIEDGEKIYEENKVG

Sequences:

>Translated_298_residues
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKSIMLEIPYSLDEITNIVVETI
RQNKLSNGYIRLVVSRGAGNLGLDPDSCTKPNVVVIAEQLSLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNI
LVRIEAKLAGVQEALMLNDQGYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVIEDGEKIYEENKVG
>Mature_298_residues
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKSIMLEIPYSLDEITNIVVETI
RQNKLSNGYIRLVVSRGAGNLGLDPDSCTKPNVVVIAEQLSLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNI
LVRIEAKLAGVQEALMLNDQGYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVIEDGEKIYEENKVG

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=38.7959866220736, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1787338, Length=249, Percent_Identity=25.7028112449799, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33138; Mature: 33138

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKS
CCCCEEEECCEECCCCCCEEEEEECCEEEECCHHCCEEEECCCHHHHHHHHHHHHHHHHH
IMLEIPYSLDEITNIVVETIRQNKLSNGYIRLVVSRGAGNLGLDPDSCTKPNVVVIAEQL
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHH
SLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNILVRIEAKLAGVQEALMLNDQ
HCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHEEEEEEHHHHCHHHHEEECCC
GYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
CEEEECCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEE
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVIEDGEKIYEENKVG
ECEEEEECCCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKS
CCCCEEEECCEECCCCCCEEEEEECCEEEECCHHCCEEEECCCHHHHHHHHHHHHHHHHH
IMLEIPYSLDEITNIVVETIRQNKLSNGYIRLVVSRGAGNLGLDPDSCTKPNVVVIAEQL
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHH
SLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNILVRIEAKLAGVQEALMLNDQ
HCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHEEEEEEHHHHCHHHHEEECCC
GYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
CEEEECCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEE
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVIEDGEKIYEENKVG
ECEEEEECCCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]