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The map label for this gene is hisH [H]

Identifier: 227815744

GI number: 227815744

Start: 2886211

End: 2886840

Strand: Reverse

Name: hisH [H]

Synonym: BAMEG_3167

Alternate gene names: 227815744

Gene position: 2886840-2886211 (Counterclockwise)

Preceding gene: 227815745

Following gene: 227815743

Centisome position: 55.2

GC content: 35.24

Gene sequence:

>630_bases
TTGATTGCCATTATAGATTATGGAATGGGAAATATTCGTAGTGTAGAACAAGCATTAAAATACATTGGAGCAGCGTACAT
CGTAACGAGTGATAAAGAAGAGATTTTTAGAAGTGATGGAGTGATTTTACCAGGAGTAGGTGCATTTCCAAAAGCTATGG
ATATATTGGAAGAAAAAGATTTAGTTCGTATGTTACAAGAAATTGGGCGTTCAAGAAAACCACTTCTAGGCATTTGCTTA
GGAATGCAGCTTTTATTTGAAAAAAGTGAGGAACTCCAAGATTGTAACGGATTAAGTTTATTGCCAGGTGTTATTCGAAA
GTTAAAAGTTCCTTATAAAATTCCTCATATGGGATGGAATGAGTTAAAGAAAGAAGGAGAAATAGCGCTTTGGAATGGAG
TAGAGGACGGTTCTTTCGTATATTATGTCCACTCTTATTACGCAGATTGTCCAAATGAAATTGTGTATGGAATAAGTGAT
TATGGAGTGAAAGTACCTGGTTTTGTAGCAAAAGGAAATATATATGGTGCGCAGTTTCATCCTGAAAAAAGTGGTGACAT
TGGCATGCAAATGTTGAAAAATTTTAAAGGAGTGGTAGAAACATGGAAATCTTCCCAGCTATCGATTTAA

Upstream 100 bases:

>100_bases
AATTGAAGCATTATTTAAAGCGTTTGGTAGAGCGCTTAGAGAAGCAGTCGAAAGAAATGCCCACATTACTGGTGTAAATT
CAACGAAAGGGATGTTGTAA

Downstream 100 bases:

>100_bases
AAGAAGGGCGATGCGTGAGACTGTATCAAGGCGAGTTTAGTAAAGAAACAGTAATGAATGAGGACCCGGTTGCGCAAGCG
ATTATATTTGAAAAATTTGG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKDLVRMLQEIGRSRKPLLGICL
GMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWNELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISD
YGVKVPGFVAKGNIYGAQFHPEKSGDIGMQMLKNFKGVVETWKSSQLSI

Sequences:

>Translated_209_residues
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKDLVRMLQEIGRSRKPLLGICL
GMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWNELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISD
YGVKVPGFVAKGNIYGAQFHPEKSGDIGMQMLKNFKGVVETWKSSQLSI
>Mature_209_residues
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKDLVRMLQEIGRSRKPLLGICL
GMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWNELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISD
YGVKVPGFVAKGNIYGAQFHPEKSGDIGMQMLKNFKGVVETWKSSQLSI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=39.5, Blast_Score=134, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23318; Mature: 23318

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKD
CEEEEECCCCCHHHHHHHHHHHCEEEEEECCHHHHHHCCCEEECCCCCCHHHHHHHHHHH
LVRMLQEIGRSRKPLLGICLGMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCHH
ELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISDYGVKVPGFVAKGNIYGAQFH
HHHCCCCEEEECCCCCCCEEEEEEHHHHCCCHHHEECHHHCCEECCCEEECCCEEECEEC
PEKSGDIGMQMLKNFKGVVETWKSSQLSI
CCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKD
CEEEEECCCCCHHHHHHHHHHHCEEEEEECCHHHHHHCCCEEECCCCCCHHHHHHHHHHH
LVRMLQEIGRSRKPLLGICLGMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCHH
ELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISDYGVKVPGFVAKGNIYGAQFH
HHHCCCCEEEECCCCCCCEEEEEEHHHHCCCHHHEECHHHCCEECCCEEECCCEEECEEC
PEKSGDIGMQMLKNFKGVVETWKSSQLSI
CCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA