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The map label for this gene is ansA1 [H]

Identifier: 227815657

GI number: 227815657

Start: 2804069

End: 2805001

Strand: Direct

Name: ansA1 [H]

Synonym: BAMEG_3076

Alternate gene names: 227815657

Gene position: 2804069-2805001 (Clockwise)

Preceding gene: 227815653

Following gene: 227815671

Centisome position: 53.61

GC content: 37.08

Gene sequence:

>933_bases
ATGGAGGAAGATAAAGAAACTGGGGTCGTACAACCAGGTGAAAAAAATCCTCTTTTAAAATTCATTCCTGATTTAGAAGG
CGATGTTGATTTAATCGTTGAAGATGTGTTCCATCTACCATCTCCTCATATGACACCAAGCGAAATGTTACAATTACAAG
TCATCATTGATGAAAGAGTAAAACAAGACAACATTCATGGCGTAGTCATTACGCATGGTACGGATACATTAGAAGAAACA
GCCTATTTCTTAGATTTAACAGTACAAGCAACTATTCCGATCGTTGTAACAGGTGCAATGCGATCTAGTAATGAATTAGG
TGCTGATGGTCTATATAACTTCTTATCAGCTGTAAAAGTCGCTAGCAGTAGTGAAGCCGCAGAAAAAGGTGTTTTAGTCG
TATTAAACGACGAAATTCATTGCGCTACAAACGTAACGAAAACACATACAAGCAATGTAGCAACATTCCAAAGTCCACAG
TATGGACCAATTGGTATGGTAACAAAACGCGGTGTTGTATTCCACCATGCTTTAGTACATCACGAAACATATTCAGTAAA
TAAGGTTTCTAAAAACGTAGTCGTTCTAAAAGCATACGCAGGCATGGACGATACATTGTTAGCAGCAATTGAAAACCTTC
CAGTAGACGGGATTGTTATTGAAGCACTCGGACAAGGTAATTTACCTCCAAGAACACTTCCTAGTCTAGAACGATTAATA
AACAAAGGAATTCCTGTCGTATTAGTTTCTCGCTGTTTCAACGGCATCGTTCAAGATGTATATTCATATGAAGGCGGCGG
CAAACAATTAAAAGATATGGGTATTGTATTCACATACGGTTTAAATGCTCAAAAAGCACGTATTAAATTATTAGTTGCAT
TAGAGAATACAACTTCTCATGAAGCAATCCAAAATATGTTTATGCACGATTAA

Upstream 100 bases:

>100_bases
ATTTACTCAAAACATGTTATGTTCTAAATGTATTTTTTACTTGAAAGGGGTATAAGCATTGAAAAGAATCCTAGTTTTAC
ACACAGGTGGAACAATTGCA

Downstream 100 bases:

>100_bases
AAAAGAAGCTGTCGAAGTTTATTTCGACAGCTTCTTTTTATATTTCTCTTGTCGCAATGGTTTGCGCAATTGCATCTCCA
TGAAATCGACCGTTCTCAAT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHEAIQNMFMHD

Sequences:

>Translated_310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHEAIQNMFMHD
>Mature_310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHEAIQNMFMHD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=235, Percent_Identity=28.5106382978723, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789327, Length=299, Percent_Identity=36.1204013377926, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1788065, Length=233, Percent_Identity=26.6094420600858, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=236, Percent_Identity=26.271186440678, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17558278, Length=236, Percent_Identity=26.271186440678, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=274, Percent_Identity=25.9124087591241, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=236, Percent_Identity=26.271186440678, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323189, Length=304, Percent_Identity=29.6052631578947, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323187, Length=304, Percent_Identity=29.6052631578947, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323186, Length=304, Percent_Identity=29.6052631578947, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323184, Length=304, Percent_Identity=29.6052631578947, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320527, Length=299, Percent_Identity=29.0969899665552, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24645530, Length=238, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24639609, Length=233, Percent_Identity=24.0343347639485, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33792; Mature: 33792

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERV
CCCCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHEEEEEEEHHHC
KQDNIHGVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKV
CCCCCCEEEEECCCCCHHHHEEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
ASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVH
CCCCCHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHH
HETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHH
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSH
HCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCEEEEEEEEECCCCHH
EAIQNMFMHD
HHHHHHHCCC
>Mature Secondary Structure
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERV
CCCCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHEEEEEEEHHHC
KQDNIHGVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKV
CCCCCCEEEEECCCCCHHHHEEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
ASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVH
CCCCCHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHH
HETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLI
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHH
NKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSH
HCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCEEEEEEEEECCCCHH
EAIQNMFMHD
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]