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The map label for this gene is fliF [H]

Identifier: 227815496

GI number: 227815496

Start: 2654408

End: 2655790

Strand: Reverse

Name: fliF [H]

Synonym: BAMEG_2909

Alternate gene names: 227815496

Gene position: 2655790-2654408 (Counterclockwise)

Preceding gene: 227815497

Following gene: 227815495

Centisome position: 50.78

GC content: 37.09

Gene sequence:

>1383_bases
ATGGAAAAGATGAAAAATGTTATCCAATCGTTAAAAACGTGGCATAAGTTAGTAATCGGTGCTGCGCTTTTAGCGATTGT
AACAGGAGCACTTTTATACTTCACCTTGCCAGATAAATATGTTGTTGTATATCAAAATTTAAATGATGCAGATAAGCAAG
AGATTACAGCAGAATTATCAAAGTTAGGTGTCGATTATCAGTTAGCGGCCGATGGATCGATTCGTGTGCAAAAAAATGAT
GCTCCATGGGTGCGAAAAGAAATGAATGGGATGGGCTTACCGTTTAATTCTAAAAGCGGTGAGGAAATTTTATTAGAAAG
CTCGCTCGGTTCAAGTGAGCAAGATAAAAAAATGAAGCAAATTGTCGGTACGAAAAAGCAATTGGAGCAAGATATTGTAA
GAAACTTTGCGACAGTTGAAACGGCGAATGTTCAAATTACATTACCTGAAAAAGAGACAATTTTTGATGAAGAAAAAGCA
AAAGGAACAGCTGCGATTACTGTTGGTGTAAAGCGTGGTCAATTATTAACTGCTGATCAAGTTGCGGGTATACAGCAAAT
GATTAGTGCAGCAGTTCCTGGGGTGAAAGCAGAAGAAGTAAGTGTGATTGATAGTAAAAAAGGTGTCATCTCAAAAGGGG
CAGATGAAGCGCATTCTACTAGTTCCTCTTCTTATGAAAAAGAAGTAGAGATGCAACATCAAATTGAAGGTAAATTAAAG
CAAGATATTGATGCAACGTTAATGACGATGTTTAAACCGAATGAATATAAAGTGAATACGAAAGTATCTGTAAACTATGA
TGAAGTTACACGTCAGTCAGAAAAGTATGGTGATAAAGGTGTACTTCGTAGTAAACAAGAGCAAGAAGAAAGCTCTACTG
CACAAGAAGGTGCAGAGACGAAGCAAGGCGCTGGTATTACAGCGAACGGTGAAGTACCAAACTATGGTACGAACAATAAT
CAAAATGGTAAAATCGTCTACGATAATAAAAATGGTAACAAAATTGAAAACTATGAAATAGATAAAACAGTTGAAACAAT
TAAGAAACACCCAGAATTAACGAAAACAAATGTTGTAGTATGGGTAGACAACGATACGTTAGTAAAACGAAAAATAGATA
TGACTACTTTCAAAGAAGCAATCGGCACAGCTGCTGGGCTTCAAGCTGATCCGAATGGAAACTTTACAAACGGTCAAGTT
AACGTTGTAACTGTTCAGTTTGATCAGCCGAAAGAAGAGAAGAAGAAAGAACCAGAAGAAAGCGGCATAAACTGGTGGTT
ATTCGGTGGAATTCCAGCTGGTTTATTAGCGATTGGTGGTCTAGTATGGTTCTTCTTAGCAAGACGTAAGAGAAAGAAGA
AGAGGAATATGAAGAATACTTAG

Upstream 100 bases:

>100_bases
TCTCAAATGAAAACGGCTGCTCTCGTACGTGATAATCTTATTGAAAATTATAAGTCACTAATTAATATGCAAATTTAGGA
AAGTGGACGGGTGTGTTTAA

Downstream 100 bases:

>100_bases
CAGAAGAGGAAATTGCTGCAAGTAATGAAAGTATTATGGAAATTCCTGAAGAGAAAATAGTACCAGAGCCAAAACCAGAA
CCAGAAGAACCGAAAGAACC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MEKMKNVIQSLKTWHKLVIGAALLAIVTGALLYFTLPDKYVVVYQNLNDADKQEITAELSKLGVDYQLAADGSIRVQKND
APWVRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQIVGTKKQLEQDIVRNFATVETANVQITLPEKETIFDEEKA
KGTAAITVGVKRGQLLTADQVAGIQQMISAAVPGVKAEEVSVIDSKKGVISKGADEAHSTSSSSYEKEVEMQHQIEGKLK
QDIDATLMTMFKPNEYKVNTKVSVNYDEVTRQSEKYGDKGVLRSKQEQEESSTAQEGAETKQGAGITANGEVPNYGTNNN
QNGKIVYDNKNGNKIENYEIDKTVETIKKHPELTKTNVVVWVDNDTLVKRKIDMTTFKEAIGTAAGLQADPNGNFTNGQV
NVVTVQFDQPKEEKKKEPEESGINWWLFGGIPAGLLAIGGLVWFFLARRKRKKKRNMKNT

Sequences:

>Translated_460_residues
MEKMKNVIQSLKTWHKLVIGAALLAIVTGALLYFTLPDKYVVVYQNLNDADKQEITAELSKLGVDYQLAADGSIRVQKND
APWVRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQIVGTKKQLEQDIVRNFATVETANVQITLPEKETIFDEEKA
KGTAAITVGVKRGQLLTADQVAGIQQMISAAVPGVKAEEVSVIDSKKGVISKGADEAHSTSSSSYEKEVEMQHQIEGKLK
QDIDATLMTMFKPNEYKVNTKVSVNYDEVTRQSEKYGDKGVLRSKQEQEESSTAQEGAETKQGAGITANGEVPNYGTNNN
QNGKIVYDNKNGNKIENYEIDKTVETIKKHPELTKTNVVVWVDNDTLVKRKIDMTTFKEAIGTAAGLQADPNGNFTNGQV
NVVTVQFDQPKEEKKKEPEESGINWWLFGGIPAGLLAIGGLVWFFLARRKRKKKRNMKNT
>Mature_460_residues
MEKMKNVIQSLKTWHKLVIGAALLAIVTGALLYFTLPDKYVVVYQNLNDADKQEITAELSKLGVDYQLAADGSIRVQKND
APWVRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQIVGTKKQLEQDIVRNFATVETANVQITLPEKETIFDEEKA
KGTAAITVGVKRGQLLTADQVAGIQQMISAAVPGVKAEEVSVIDSKKGVISKGADEAHSTSSSSYEKEVEMQHQIEGKLK
QDIDATLMTMFKPNEYKVNTKVSVNYDEVTRQSEKYGDKGVLRSKQEQEESSTAQEGAETKQGAGITANGEVPNYGTNNN
QNGKIVYDNKNGNKIENYEIDKTVETIKKHPELTKTNVVVWVDNDTLVKRKIDMTTFKEAIGTAAGLQADPNGNFTNGQV
NVVTVQFDQPKEEKKKEPEESGINWWLFGGIPAGLLAIGGLVWFFLARRKRKKKRNMKNT

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=373, Percent_Identity=24.1286863270777, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 50924; Mature: 50924

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMKNVIQSLKTWHKLVIGAALLAIVTGALLYFTLPDKYVVVYQNLNDADKQEITAELS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCHHHHHHHHHH
KLGVDYQLAADGSIRVQKNDAPWVRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQ
HCCCCEEEECCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
IVGTKKQLEQDIVRNFATVETANVQITLPEKETIFDEEKAKGTAAITVGVKRGQLLTADQ
HHCCHHHHHHHHHHHHHHEEECEEEEEECCCHHHCCHHHCCCEEEEEEEECCCCEECHHH
VAGIQQMISAAVPGVKAEEVSVIDSKKGVISKGADEAHSTSSSSYEKEVEMQHQIEGKLK
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHH
QDIDATLMTMFKPNEYKVNTKVSVNYDEVTRQSEKYGDKGVLRSKQEQEESSTAQEGAET
HHHHHHHHHEECCCCEEEEEEEEECHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCH
KQGAGITANGEVPNYGTNNNQNGKIVYDNKNGNKIENYEIDKTVETIKKHPELTKTNVVV
HCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEE
WVDNDTLVKRKIDMTTFKEAIGTAAGLQADPNGNFTNGQVNVVTVQFDQPKEEKKKEPEE
EECCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHCCCHH
SGINWWLFGGIPAGLLAIGGLVWFFLARRKRKKKRNMKNT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKMKNVIQSLKTWHKLVIGAALLAIVTGALLYFTLPDKYVVVYQNLNDADKQEITAELS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCHHHHHHHHHH
KLGVDYQLAADGSIRVQKNDAPWVRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQ
HCCCCEEEECCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
IVGTKKQLEQDIVRNFATVETANVQITLPEKETIFDEEKAKGTAAITVGVKRGQLLTADQ
HHCCHHHHHHHHHHHHHHEEECEEEEEECCCHHHCCHHHCCCEEEEEEEECCCCEECHHH
VAGIQQMISAAVPGVKAEEVSVIDSKKGVISKGADEAHSTSSSSYEKEVEMQHQIEGKLK
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHH
QDIDATLMTMFKPNEYKVNTKVSVNYDEVTRQSEKYGDKGVLRSKQEQEESSTAQEGAET
HHHHHHHHHEECCCCEEEEEEEEECHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCH
KQGAGITANGEVPNYGTNNNQNGKIVYDNKNGNKIENYEIDKTVETIKKHPELTKTNVVV
HCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEE
WVDNDTLVKRKIDMTTFKEAIGTAAGLQADPNGNFTNGQVNVVTVQFDQPKEEKKKEPEE
EECCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHCCCHH
SGINWWLFGGIPAGLLAIGGLVWFFLARRKRKKKRNMKNT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]