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The map label for this gene is yodT [H]

Identifier: 227814889

GI number: 227814889

Start: 2096646

End: 2097956

Strand: Reverse

Name: yodT [H]

Synonym: BAMEG_2300

Alternate gene names: 227814889

Gene position: 2097956-2096646 (Counterclockwise)

Preceding gene: 227814890

Following gene: 227814888

Centisome position: 40.11

GC content: 40.81

Gene sequence:

>1311_bases
ATGCGCGATTACTTAATTAAACCACTTGTTGGTCAGCCGTATCCAATGATTTCACATGGAAAAGGTGTGTATTTGTATGA
TCAAAACGGAAATAAATATTTTGATGGCTCGTCAGGGGCAATTACGGCAGGGATTGGGCATGGCGTAAAGGAGATTGCGG
ACGTTATCAAAAAGCAAGCAGAGGAGATTGCTTTCGTTTATAGATCACAGTTTACGAGTGAACCAGCTGAAAAATTAGCG
AAGAAATTAAGCGATTTAAGTGTAGGAGATTTGAACTGGAGCTTTTTTGTAAATAGTGGTACGGAAGCAAATGAAACAGC
TATGAAAATTGCAATTCAGCATTTTCAAGAGCGGGGTATTCAAGGAAAGCATAAAATTTTGTCTCGATGGATGAGTTATC
ACGGCATTACGATGGGAGCATTATCGATGTCTGGGCATCCACTCCGTAGACAACGTTTCGTATCAATTTTAGAAGATTAT
CCAACTATTCCAGCTCCATATTGTTTTAGATGTCCGGTGCAAAAAGTATATCCAACTTGTCAGCTTGCCTGTGCGACGGA
ACTAGAAAGGTCAATTGAAAGAATTGGAGCAGAACATATTGCAGCTTTTATCGCTGAACCGATTATTGGAGCAGCTGGGG
GCGCAGTCGTTCCGCCGAAAGAATATTATAAAGTTATTAAAGATATTTGTAGCCATTACGATATTTTATTTATTGCTGAT
GAAGTAATGACTGGGCTTGGTCGTACTGGTGCATGGTTTGCGATGGAGCATTGGGGTGTAGAACCGGATATTATGACTCT
TGGTAAAGGTTTAGGAGCTGGATACACACCGATGGCAGCGACGGTTGTAAGTGACCGTGTTATGGAGCCGATTTTACGAG
GGTCACGTTCTGTTATGAGTGGGCATACGTTAAGTGCAAATCCATTATCTGCAGCAACAGCTCTAGCTGTTATTGAATAT
ATGGAGAAACATAATCTACCTGAAAAAACAGCGGAAAAGGGAGAGTATTTAATAAAGGGACTACAGAAAGTTCAGCAACA
ATCGACAATCATTGCTGATGTGCGCGGAAAAGGATTGCTAATCGGAGTAGAATTGCAACCGTTTTCAAAAGCGTCGGAAC
TTATTTCGGTTGCAGCTAAAAATGGTCTTCTTTTATACCAAGCTGTTTCAGGGCAAGCAGGAAAAGAAGATAGTGCACTG
CTTGTGGCACCGCCAATGACAACTACATATTCCGAGTTAGATGAATTACTTTCAATTTTTGCAAAAAGTGTAGAAGAAAT
GATGCAAAAAGGAGGGCATAGTATCGCATGA

Upstream 100 bases:

>100_bases
TTTTTGATAAATTCACTGCTTTCGCAGAGGGTTCTTTTTTATGAAAGAAACGTCAATTTTCTTCATATATATATACAAAT
TGGAGAAATGGGGGAGCGCT

Downstream 100 bases:

>100_bases
CAACTATTACGAATACATTCGATAAATTAAAAGAGATAGAGGAAGTAATTTCTTTATTCCACGATGATATGACATTAATG
TTTGGGGGATTTGGGGGGAT

Product: hypothetical protein

Products: N-Acetyl-L-glutamate 5-semialdehyde; L-Glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRDYLIKPLVGQPYPMISHGKGVYLYDQNGNKYFDGSSGAITAGIGHGVKEIADVIKKQAEEIAFVYRSQFTSEPAEKLA
KKLSDLSVGDLNWSFFVNSGTEANETAMKIAIQHFQERGIQGKHKILSRWMSYHGITMGALSMSGHPLRRQRFVSILEDY
PTIPAPYCFRCPVQKVYPTCQLACATELERSIERIGAEHIAAFIAEPIIGAAGGAVVPPKEYYKVIKDICSHYDILFIAD
EVMTGLGRTGAWFAMEHWGVEPDIMTLGKGLGAGYTPMAATVVSDRVMEPILRGSRSVMSGHTLSANPLSAATALAVIEY
MEKHNLPEKTAEKGEYLIKGLQKVQQQSTIIADVRGKGLLIGVELQPFSKASELISVAAKNGLLLYQAVSGQAGKEDSAL
LVAPPMTTTYSELDELLSIFAKSVEEMMQKGGHSIA

Sequences:

>Translated_436_residues
MRDYLIKPLVGQPYPMISHGKGVYLYDQNGNKYFDGSSGAITAGIGHGVKEIADVIKKQAEEIAFVYRSQFTSEPAEKLA
KKLSDLSVGDLNWSFFVNSGTEANETAMKIAIQHFQERGIQGKHKILSRWMSYHGITMGALSMSGHPLRRQRFVSILEDY
PTIPAPYCFRCPVQKVYPTCQLACATELERSIERIGAEHIAAFIAEPIIGAAGGAVVPPKEYYKVIKDICSHYDILFIAD
EVMTGLGRTGAWFAMEHWGVEPDIMTLGKGLGAGYTPMAATVVSDRVMEPILRGSRSVMSGHTLSANPLSAATALAVIEY
MEKHNLPEKTAEKGEYLIKGLQKVQQQSTIIADVRGKGLLIGVELQPFSKASELISVAAKNGLLLYQAVSGQAGKEDSAL
LVAPPMTTTYSELDELLSIFAKSVEEMMQKGGHSIA
>Mature_436_residues
MRDYLIKPLVGQPYPMISHGKGVYLYDQNGNKYFDGSSGAITAGIGHGVKEIADVIKKQAEEIAFVYRSQFTSEPAEKLA
KKLSDLSVGDLNWSFFVNSGTEANETAMKIAIQHFQERGIQGKHKILSRWMSYHGITMGALSMSGHPLRRQRFVSILEDY
PTIPAPYCFRCPVQKVYPTCQLACATELERSIERIGAEHIAAFIAEPIIGAAGGAVVPPKEYYKVIKDICSHYDILFIAD
EVMTGLGRTGAWFAMEHWGVEPDIMTLGKGLGAGYTPMAATVVSDRVMEPILRGSRSVMSGHTLSANPLSAATALAVIEY
MEKHNLPEKTAEKGEYLIKGLQKVQQQSTIIADVRGKGLLIGVELQPFSKASELISVAAKNGLLLYQAVSGQAGKEDSAL
LVAPPMTTTYSELDELLSIFAKSVEEMMQKGGHSIA

Specific function: Involved In Both The Arginine And Lysine Biosynthetic Pathways. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=420, Percent_Identity=30.7142857142857, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI13994255, Length=459, Percent_Identity=29.1938997821351, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI37574042, Length=421, Percent_Identity=28.5035629453682, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI226442705, Length=421, Percent_Identity=28.7410926365796, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI226442709, Length=377, Percent_Identity=28.6472148541114, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI284507298, Length=331, Percent_Identity=32.02416918429, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI24119277, Length=429, Percent_Identity=27.039627039627, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI188536080, Length=440, Percent_Identity=25.2272727272727, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38679950, Length=440, Percent_Identity=25.2272727272727, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38679946, Length=440, Percent_Identity=25.2272727272727, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789759, Length=428, Percent_Identity=28.9719626168224, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI145693181, Length=407, Percent_Identity=29.4840294840295, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788044, Length=422, Percent_Identity=29.3838862559242, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1789016, Length=435, Percent_Identity=26.8965517241379, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1786991, Length=422, Percent_Identity=30.3317535545024, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787560, Length=420, Percent_Identity=28.0952380952381, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786349, Length=362, Percent_Identity=29.2817679558011, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32564660, Length=444, Percent_Identity=30.8558558558559, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25144271, Length=429, Percent_Identity=27.7389277389277, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI25144274, Length=300, Percent_Identity=29.3333333333333, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71992977, Length=439, Percent_Identity=25.7403189066059, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17541228, Length=437, Percent_Identity=24.4851258581236, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=425, Percent_Identity=28.9411764705882, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324432, Length=426, Percent_Identity=30.9859154929577, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324386, Length=305, Percent_Identity=30.4918032786885, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28574759, Length=461, Percent_Identity=30.58568329718, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI161085790, Length=467, Percent_Identity=30.1927194860814, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21357415, Length=419, Percent_Identity=28.4009546539379, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI21356575, Length=434, Percent_Identity=28.3410138248848, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281366494, Length=379, Percent_Identity=22.9551451187335, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667139, Length=379, Percent_Identity=22.9551451187335, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667143, Length=379, Percent_Identity=22.9551451187335, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.11 [C]

Molecular weight: Translated: 47538; Mature: 47538

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDYLIKPLVGQPYPMISHGKGVYLYDQNGNKYFDGSSGAITAGIGHGVKEIADVIKKQA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCEEEEHHCCCHHHHHHHHHHHH
EEIAFVYRSQFTSEPAEKLAKKLSDLSVGDLNWSFFVNSGTEANETAMKIAIQHFQERGI
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
QGKHKILSRWMSYHGITMGALSMSGHPLRRQRFVSILEDYPTIPAPYCFRCPVQKVYPTC
CHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCHH
QLACATELERSIERIGAEHIAAFIAEPIIGAAGGAVVPPKEYYKVIKDICSHYDILFIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHH
EVMTGLGRTGAWFAMEHWGVEPDIMTLGKGLGAGYTPMAATVVSDRVMEPILRGSRSVMS
HHHHCCCCCCCEEEHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHC
GHTLSANPLSAATALAVIEYMEKHNLPEKTAEKGEYLIKGLQKVQQQSTIIADVRGKGLL
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEE
IGVELQPFSKASELISVAAKNGLLLYQAVSGQAGKEDSALLVAPPMTTTYSELDELLSIF
EEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
AKSVEEMMQKGGHSIA
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRDYLIKPLVGQPYPMISHGKGVYLYDQNGNKYFDGSSGAITAGIGHGVKEIADVIKKQA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCEEEEHHCCCHHHHHHHHHHHH
EEIAFVYRSQFTSEPAEKLAKKLSDLSVGDLNWSFFVNSGTEANETAMKIAIQHFQERGI
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
QGKHKILSRWMSYHGITMGALSMSGHPLRRQRFVSILEDYPTIPAPYCFRCPVQKVYPTC
CHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCHH
QLACATELERSIERIGAEHIAAFIAEPIIGAAGGAVVPPKEYYKVIKDICSHYDILFIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHH
EVMTGLGRTGAWFAMEHWGVEPDIMTLGKGLGAGYTPMAATVVSDRVMEPILRGSRSVMS
HHHHCCCCCCCEEEHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHC
GHTLSANPLSAATALAVIEYMEKHNLPEKTAEKGEYLIKGLQKVQQQSTIIADVRGKGLL
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEE
IGVELQPFSKASELISVAAKNGLLLYQAVSGQAGKEDSALLVAPPMTTTYSELDELLSIF
EEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
AKSVEEMMQKGGHSIA
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate [C]

Metal ions: Ni2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.1 {N2-acetyl-L-ornithine}} 1.54 {N2-acetyl-L-ornithine}} 1.3 {N2-acetyl-L-ornithine}} 1.1 {N2-acetyl-L-ornithine}} 0.48 {N2-acetyl-L-ornithine}} 0.34 {N2-acetyl-L-ornithine}} 2.5 {2-oxoglutarate}} [C]

Substrates: N2-Acetyl-L-ornithine; 2-Oxoglutarate [C]

Specific reaction: N2-Acetyl-L-ornithine + 2-Oxoglutarate <==> N-Acetyl-L-glutamate 5-semialdehyde + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: Cu2+; Ornithine; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]