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The map label for this gene is iolD1 [H]

Identifier: 227814677

GI number: 227814677

Start: 1900141

End: 1902075

Strand: Reverse

Name: iolD1 [H]

Synonym: BAMEG_2087

Alternate gene names: 227814677

Gene position: 1902075-1900141 (Counterclockwise)

Preceding gene: 227814678

Following gene: 227814676

Centisome position: 36.37

GC content: 38.86

Gene sequence:

>1935_bases
ATGCAAACTGTTAGAATGACAACGGCGCAAGCATTGGTAAAGTTTTTAAATCAGCAATATGTGGAGTTTGATGGAAAGCA
ACAAAAGTTTGTTAAGGGAATCTTTACGATTTTTGGTCATGGAAATGTAGTAGGCCTAGGTCAGGCTTTAGAAGAGGACG
CTGGGGAATTAGAAGTATATCAAGGGAGAAATGAGCAAGGAATGGCAAATGCTGCGATGGCTTTTGCAAAACAAAAACAT
AGAAAACAAATTATGGCATGTACTTCCTCTGTTGGTCCTGGATCAGCAAATATGATTACCTCTGCAGCAACAGCTTCTGC
AAATAATATTCCCGTTTTATTACTTCCAGGAGATGTATTTGCAACGAGACAACCTGATCCTGTTCTTCAACAAATTGAAC
AAACACATGACTTATCTATTTCTACAAATGATGCTTTTCGTGCAGTAAGTAAGTACTGGGACCGGATAAATCGTCCTGAA
CAATTGATGACCGCTATGATTCAAGCGATGCGTGTTTTAACGAATCCGGCAGATACAGGAGCTGTAACCATTTGTTTACC
ACAAGATGTCCAAGGAGAAGCATGGGATTTTCCAAGTTACTTCTTCCAAAAGCGTGTTCACCGTATTGAACGCCGTCTAC
CTACAAAAGCCAGCTTAGCGGATGCAGTGGAAATGATTAAGAGAAAGAAAAAGCCAGTTATGATTTGCGGTGGTGGTGTA
AGATACGCAGAAGCGGCAGAAGAGTTAAAACAGTTTGCTGAAACATTCCACATTCCATTTGGAGAAACACAAGCTGGAAA
AAGTGCGATTGAAAGTAGTCATCCATATAATCTAGGCGGCATTGGGGTAACGGGAAATGTAGCAGCTAATACAATTGCAA
AGGAAGCGGATCTTGTTATTGGCATCGGGACGAGATTTACTGATTTTACAACGGCATCTAAACAATTATTTCAAAATGAA
GAAGTTGAGTTTTTAAACATCAACATTTCAGAATTCCATGCGAATAAGCTGGATGCATTAAAGGTTATAGCGGACGCGAA
AGAAGCACTTCTTGCTCTAATAGATGAACTGCAAGAAATTGATTATCAATCTAGTTATACAGTAGAAATTGCTGATGCAA
AAGATGCTTGGGAAACAGAATTATCACGTCTACATAATATTCGCTTTACATGTCAAGATTTCACACCAGAAGTCGAAGGT
CATTTCAATGAAAATTTAAATGAGTATGTAGACGCACTTGGTACACAATTAACACAGACTGCAGTCATTGGACAAATCAA
TACATTACTTGATAAAGATGCAATTATTGTTGGAGCAGCAGGAAGTCTTCCAGGTGATTTACAAAGAATGTGGGCATCGA
GAAAACCTAATACATATCACATGGAGTATGGATACTCTTGCATGGGTTATGAGGTTGCGGGCGCACTTGGTGCAAAGCTA
GCTGAACCATCGAAAGAAGTATATGCAATGGTAGGGGATGGTAGTTACCAGATGCTTCATTCTGAGCTTGTCACAAGTCT
TCAAGAAAACAAAAAAATTAACGTCTTATTGTTTGATAACTCCGGATTTGGCTGCATTAATAACTTACAAATGGGCAATG
GAATGGGGAGCTTTGGAACAGAGTTTCGCTACCGAAACCAGGAAACACGTAAATTAGACGGAGCAATTATGAAAATCGAT
TTTGCAGCTAGCGCAGCTGGATATGGTGTGAAAACATATCATGTTACATCACTAGAACAATTACAAGAAGCGCTTATAGA
TGCGAAAAAACAAACAGTTTCTACATTAATTGATATTAAAGTATTACCCAAAACAATGACAAATGGATACGAGTCATGGT
GGCATGTAGGTGTTGCAGAAGTATCTAAAAATCAAAGTGTACAAGCTGCGTACGAGAGTAAAGTAAGTAACTTGCAACAG
GCAAGATCTTATTAG

Upstream 100 bases:

>100_bases
GTAGTAAAACATAAGAAGGAAGTATTAGGAGTTTATCGAATTCCTGATACTCCTTTTTCATATGCAGAATGACTGTTAGA
GATAGAAAGGAGCAAATCAT

Downstream 100 bases:

>100_bases
ATTAGGGCACAAAGAGAGCTTTTTATCTACATGCATATTGTTTAAGAAGCTCTCTTTTTAATGAACAGATAAGAGAATTT
GGCGAAATTCTATCACATTT

Product: putative iolD protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFVKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNVAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFLNINISEFHANKLDALKVIADAKEALLALIDELQEIDYQSSYTVEIADAKDAWETELSRLHNIRFTCQDFTPEVEG
HFNENLNEYVDALGTQLTQTAVIGQINTLLDKDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGAIMKID
FAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGVAEVSKNQSVQAAYESKVSNLQQ
ARSY

Sequences:

>Translated_644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFVKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNVAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFLNINISEFHANKLDALKVIADAKEALLALIDELQEIDYQSSYTVEIADAKDAWETELSRLHNIRFTCQDFTPEVEG
HFNENLNEYVDALGTQLTQTAVIGQINTLLDKDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGAIMKID
FAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGVAEVSKNQSVQAAYESKVSNLQQ
ARSY
>Mature_644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFVKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNVAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFLNINISEFHANKLDALKVIADAKEALLALIDELQEIDYQSSYTVEIADAKDAWETELSRLHNIRFTCQDFTPEVEG
HFNENLNEYVDALGTQLTQTAVIGQINTLLDKDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGAIMKID
FAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGVAEVSKNQSVQAAYESKVSNLQQ
ARSY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=20.7815275310835, Blast_Score=93, Evalue=9e-19,
Organism=Escherichia coli, GI1790104, Length=604, Percent_Identity=24.1721854304636, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=24.9116607773852, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI87081685, Length=584, Percent_Identity=24.6575342465753, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787096, Length=527, Percent_Identity=22.5806451612903, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788716, Length=532, Percent_Identity=21.4285714285714, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531299, Length=449, Percent_Identity=24.2761692650334, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531301, Length=449, Percent_Identity=24.2761692650334, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=592, Percent_Identity=22.972972972973, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922626, Length=619, Percent_Identity=22.7786752827141, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 71014; Mature: 71014

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFVKGIFTIFGHGNVVGLGQALEEDAGELEVY
CCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMANAAMAFAKQKHRKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHCCCCCCCEEEEECCCEE
ATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPEQLMTAMIQAMRVLTNPADTG
CCCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCC
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNVAANTIAKEADLVI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEE
GIGTRFTDFTTASKQLFQNEEVEFLNINISEFHANKLDALKVIADAKEALLALIDELQEI
ECCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DYQSSYTVEIADAKDAWETELSRLHNIRFTCQDFTPEVEGHFNENLNEYVDALGTQLTQT
CCCCCEEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVIGQINTLLDKDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
HHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEECCCEEECHHHHHHHHHHH
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGT
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCC
EFRYRNQETRKLDGAIMKIDFAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIK
CCEECCCHHHHHCCEEEEEEEHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHE
VLPKTMTNGYESWWHVGVAEVSKNQSVQAAYESKVSNLQQARSY
ECCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFVKGIFTIFGHGNVVGLGQALEEDAGELEVY
CCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMANAAMAFAKQKHRKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHCCCCCCCEEEEECCCEE
ATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPEQLMTAMIQAMRVLTNPADTG
CCCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCC
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNVAANTIAKEADLVI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEE
GIGTRFTDFTTASKQLFQNEEVEFLNINISEFHANKLDALKVIADAKEALLALIDELQEI
ECCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DYQSSYTVEIADAKDAWETELSRLHNIRFTCQDFTPEVEGHFNENLNEYVDALGTQLTQT
CCCCCEEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVIGQINTLLDKDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
HHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEECCCEEECHHHHHHHHHHH
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGT
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCC
EFRYRNQETRKLDGAIMKIDFAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIK
CCEECCCHHHHHCCEEEEEEEHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHE
VLPKTMTNGYESWWHVGVAEVSKNQSVQAAYESKVSNLQQARSY
ECCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA