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The map label for this gene is yfkN [H]

Identifier: 227814670

GI number: 227814670

Start: 1894944

End: 1895738

Strand: Reverse

Name: yfkN [H]

Synonym: BAMEG_2080

Alternate gene names: 227814670

Gene position: 1895738-1894944 (Counterclockwise)

Preceding gene: 227814671

Following gene: 227814669

Centisome position: 36.25

GC content: 35.35

Gene sequence:

>795_bases
ATGCAAAAAATGAAATGGAAGAACTATTTATGTTATTTTGTTATTTTTATACTTCTTGGAACAGCAGTAACAGTCAAACC
ATCTATCTCAAAAGCTGAGGAATCTGATGTTAACATTACACTGTTAGGTACTGCAGATATTCATGGTAGATTTATGCCTT
GGGATTATGCGCTTGATGGTGCAAATACGAGTGGAAGTTTAACGCAGCTTTATACGGTTATAAAGAAGGTGCGTCAGGAA
AATCCCAATACAATATTAGTAGATGCTGGGGATACAGTTCAAGGAAACTCAGTAGAGTTATTCAATGATCAGCCACAATC
GCCAATGATGGTAGCGATGAATGCAATGGGATATGATGCTTGGGCATTTGGAAATCATGAATTTAACTTTGGATTAGATA
CATTGAAAAAAGTTAGTGAACAATATAAGGGAAATACATTAGCAGGAAATATTTATAAGGAAAATGGAGAGCGTTTTCTT
CCTGCATATACGATTGTTGAAAAAGGTGGAATTAAAGTTGGGATTATTGGTATGAATACACCAATGATTAGTGATTTCGA
AAAAGGGACAGATCATTTGGATGGATTGGTAGTGAAAAATCCAGTAGAAGAGACAAAAAAGGCAATTAAAGAATTAGAGG
GTAAAGTAGATGTAATGGTAGGGGTTATGCATATGGGATTAGAAAATGAGAATGGAATCCCTGGTACTGGGGTTCAAGAT
ATTGCGAATGCTTGTCCGGAATTAAGTGCTATTTTTGCAGCTCATATGCATAAGCTTGTAAAAAAGAAGTTGTAA

Upstream 100 bases:

>100_bases
TGAAATTACTTTTTGTAGTTGAAGTTTTCCGAATAATAAGTTACATTTTTAAAGTTCAAGCATCTATACGACAGTACAAA
AGGGGAGAAATATTGTTATT

Downstream 100 bases:

>100_bases
ATGGTGTCATTATCACGGAACCAGATAAATATGGAACACATATCTCACGTATTGACCTTACTTTTACAAAGCAAGATGGG
AAGTTGGTTTTAAAAAATAA

Product: hypothetical protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQKMKWKNYLCYFVIFILLGTAVTVKPSISKAEESDVNITLLGTADIHGRFMPWDYALDGANTSGSLTQLYTVIKKVRQE
NPNTILVDAGDTVQGNSVELFNDQPQSPMMVAMNAMGYDAWAFGNHEFNFGLDTLKKVSEQYKGNTLAGNIYKENGERFL
PAYTIVEKGGIKVGIIGMNTPMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
IANACPELSAIFAAHMHKLVKKKL

Sequences:

>Translated_264_residues
MQKMKWKNYLCYFVIFILLGTAVTVKPSISKAEESDVNITLLGTADIHGRFMPWDYALDGANTSGSLTQLYTVIKKVRQE
NPNTILVDAGDTVQGNSVELFNDQPQSPMMVAMNAMGYDAWAFGNHEFNFGLDTLKKVSEQYKGNTLAGNIYKENGERFL
PAYTIVEKGGIKVGIIGMNTPMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
IANACPELSAIFAAHMHKLVKKKL
>Mature_264_residues
MQKMKWKNYLCYFVIFILLGTAVTVKPSISKAEESDVNITLLGTADIHGRFMPWDYALDGANTSGSLTQLYTVIKKVRQE
NPNTILVDAGDTVQGNSVELFNDQPQSPMMVAMNAMGYDAWAFGNHEFNFGLDTLKKVSEQYKGNTLAGNIYKENGERFL
PAYTIVEKGGIKVGIIGMNTPMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
IANACPELSAIFAAHMHKLVKKKL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=228, Percent_Identity=28.0701754385965, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786687, Length=258, Percent_Identity=30.6201550387597, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790658, Length=211, Percent_Identity=31.2796208530806, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 29037; Mature: 29037

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKMKWKNYLCYFVIFILLGTAVTVKPSISKAEESDVNITLLGTADIHGRFMPWDYALDG
CCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEECC
ANTSGSLTQLYTVIKKVRQENPNTILVDAGDTVQGNSVELFNDQPQSPMMVAMNAMGYDA
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCEEEEECCCCCE
WAFGNHEFNFGLDTLKKVSEQYKGNTLAGNIYKENGERFLPAYTIVEKGGIKVGIIGMNT
EEECCCCCCCCHHHHHHHHHHHCCCCEECCEECCCCCEECCEEEEEECCCEEEEEEECCC
PMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
CCCCCHHCCHHHHCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHH
IANACPELSAIFAAHMHKLVKKKL
HHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKMKWKNYLCYFVIFILLGTAVTVKPSISKAEESDVNITLLGTADIHGRFMPWDYALDG
CCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEECC
ANTSGSLTQLYTVIKKVRQENPNTILVDAGDTVQGNSVELFNDQPQSPMMVAMNAMGYDA
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCEEEEECCCCCE
WAFGNHEFNFGLDTLKKVSEQYKGNTLAGNIYKENGERFLPAYTIVEKGGIKVGIIGMNT
EEECCCCCCCCHHHHHHHHHHHCCCCEECCEECCCCCEECCEEEEEECCCEEEEEEECCC
PMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
CCCCCHHCCHHHHCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHH
IANACPELSAIFAAHMHKLVKKKL
HHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]