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The map label for this gene is yrkH [H]

Identifier: 227814651

GI number: 227814651

Start: 1873835

End: 1875289

Strand: Reverse

Name: yrkH [H]

Synonym: BAMEG_2061

Alternate gene names: 227814651

Gene position: 1875289-1873835 (Counterclockwise)

Preceding gene: 227814652

Following gene: 227814650

Centisome position: 35.86

GC content: 35.12

Gene sequence:

>1455_bases
ATGCTTTTAAAATATTTTTATGATGAAAAATTAGCACATGCTTCTTATTTAGTTGGTTGTCAGAAGGAAGGCGTAGCAAT
TGTGATTGATCCAGGTCGCTATATAGAACAATATATAGACTTTGCTAAGAAAGAGGGGATGGAAGTAATTGCTGCGGCTG
AAACTCACATTCATGCAGATTTTCTTTCTGGGTCTAGAGAACTTTCCACTCTTTATCATACCAATTTATATGTATCTGAT
GAAGGTGATTGTGATTGGAAATATCAATATCTTAACGAAGGTCGATATAATTTGGTTAGAGAGGGGACAGAGTTTAAAGT
AGGTCATATAAAATTTAATGTAATTCACACACCAGGGCATACACCTGAAAGCATTTCTTTTTTAGTAACTGATACAAGTC
AAAATAACTATACGAATGATAAGCCAATAGGAATATTCACAGGTGATTTTATATTTGTCGGAGATATAGGAAGACCCGAT
TTATTAGAAACTGCTGTAGGTATGAAAGATACCGCAAAAATAGGAGCAAAACAATTATTTGATTCGATACAAAAAATAAA
AACACTCCCTGATTATTTGCAAATATGGCCATCACATGGTGCAGGAAGCGCATGTGGAAAAGCATTAGGAGCAATTCCAA
CTTCTACCTTAGGTTATGAAAAGATGTTCAATTGGGCATTTCAGTGTAATGAAGAAAGTGATTTTATATCGACTTTATTG
GCGGGGCAGCCAGAGCCTCCAAGGTATTTTTCTTTAATGAAGAATTTAAATAAGTATGGTCCGCCCATTCGTAAGAAAAG
AGAGATTTTTGCTATTAAAACAGTAGAGGAACTTCAAGAAGTTATGAGGAGCGTTCAGCAAATAGTTGATATAAGGGATG
GAGAGAGTTTTGCTTCTGGTCACATCGAGAAATCAATTAACATACCGTGTAACAATTCCTTCACAACCTGGTGTGGATGG
TTACTAGATTATAAAACAGAAACTTTAATCATTCTAGATGAGGAAAAGGTTAGAGTGGAGGAGGTTATTAGAGACTTTGA
ATCTATTGGTCTAGATAATATTATTGCCTTTGCACCCTTACAAGTAATACAAAGACTCGATAGATTTGAAAGTTACAAAG
AAAAAACATCGATTGAATTATATCCGCATATAAAGGATGGTAGGGTTAAGGTAATTGATGTACGTAGTAAAAAAGAGTGG
GACGAAGGACATCTTCATGATGCAATACATATCCCTTTGGGAAATCTATTGAAGCAGCTAGATTGTATACCAAAGGATTG
CCCAATCGTTTTACAATGTCGAACCGGATTACGTTCCGCTATAGCAGCTAGTATTTTACAGAGAGCTGGTATAAAGGAAG
TAGTTAATCTAAAGGGAGGATTTCTTGCATGGAATAAAGAAGAGCTTCCTTATACAACTTGTAATCTCAATGTGGAGGGA
AATAATTTTTATTAA

Upstream 100 bases:

>100_bases
GTATTGGAACTTTATGTTGAAAGGAATCCTATAAGGGAAGAGAGAAAGCGGTTATAAAAATTGAAAAGCATTTTGAATTA
ACTATTTGGAGGGAATAGCT

Downstream 100 bases:

>100_bases
TTTATCCGATAAGGAAAATTACGGATTACATAGGAGTATATCTATAAATAGGAGAAATAGATAATTTATTCTATAGATCT
AAATTTATTCAGTATTATGC

Product: metallo-beta-lactamase/rhodanese-like domain protein

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIDFAKKEGMEVIAAAETHIHADFLSGSRELSTLYHTNLYVSD
EGDCDWKYQYLNEGRYNLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
AGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDGESFASGHIEKSINIPCNNSFTTWCGW
LLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEW
DEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNVEG
NNFY

Sequences:

>Translated_484_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIDFAKKEGMEVIAAAETHIHADFLSGSRELSTLYHTNLYVSD
EGDCDWKYQYLNEGRYNLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
AGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDGESFASGHIEKSINIPCNNSFTTWCGW
LLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEW
DEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNVEG
NNFY
>Mature_484_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIDFAKKEGMEVIAAAETHIHADFLSGSRELSTLYHTNLYVSD
EGDCDWKYQYLNEGRYNLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
AGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDGESFASGHIEKSINIPCNNSFTTWCGW
LLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEW
DEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNVEG
NNFY

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787158, Length=227, Percent_Identity=25.9911894273128, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17538952, Length=189, Percent_Identity=27.5132275132275, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 54883; Mature: 54883

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIDFAKKEGMEVIAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
FLSGSRELSTLYHTNLYVSDEGDCDWKYQYLNEGRYNLVREGTEFKVGHIKFNVIHTPGH
HHCCCHHHHEEEEEEEEEECCCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEEEECCCC
TPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPDLLETAVGMKDTAKIGAKQLF
CCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHH
DSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHHHHHHH
AGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDGESFASG
CCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHCC
HIEKSINIPCNNSFTTWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPL
CCCEEECCCCCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHH
QVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEWDEGHLHDAIHIPLGNLLKQL
HHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHH
DCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNVEG
HCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCEEEEEEEECC
NNFY
CCCC
>Mature Secondary Structure
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIDFAKKEGMEVIAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
FLSGSRELSTLYHTNLYVSDEGDCDWKYQYLNEGRYNLVREGTEFKVGHIKFNVIHTPGH
HHCCCHHHHEEEEEEEEEECCCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEEEECCCC
TPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPDLLETAVGMKDTAKIGAKQLF
CCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHH
DSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDFISTLL
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHHHHHHH
AGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDGESFASG
CCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHCC
HIEKSINIPCNNSFTTWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNIIAFAPL
CCCEEECCCCCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHH
QVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEWDEGHLHDAIHIPLGNLLKQL
HHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHH
DCIPKDCPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWNKEELPYTTCNLNVEG
HCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCEEEEEEEECC
NNFY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]