BacMap

Definition	Bacillus anthracis str. CDC 684, complete genome.
Accession	NC_012581
Length	5,230,115

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The map label for this gene is yngF [H]

Identifier: 227814639

GI number: 227814639

Start: 1864133

End: 1864921

Strand: Reverse

Name: yngF [H]

Synonym: BAMEG_2049

Alternate gene names: 227814639

Gene position: 1864921-1864133 (Counterclockwise)

Preceding gene: 227814640

Following gene: 227814638

Centisome position: 35.66

GC content: 37.52

Gene sequence:

>789_bases
ATGCTACAATTACAAAATATTTCAGTTGATTATGCAACACCGCACGTTGTAAAGATTTCATTAAATCGTGAAAGACAAGC
AAACTCATTATCTTTAGCGTTATTAGAAGAGTTACAAAACATATTAACTCAAATAAATGAAGAGGCAAATACTCGTGTAG
TTATTTTAACAGGTGCTGGTGAAAAAGCGTTTTGTGCTGGTGCTGATTTAAAAGAACGTGCTGGCATGAATGAAGAACAA
GTTCGCCATGCTGTTAGTATGATTCGCACTACTATGGAAATGGTTGAACAATTACCACAGCCAGTTATTGCTGCTATAAA
TGGCATCGCACTTGGTGGTGGTACGGAATTAAGTTTAGCTTGTGATTTTAGAATTGCGGCTGAATCTGCGAGTCTTGGTC
TTACTGAAACTACACTTGCAATTATACCAGGAGCAGGTGGTACGCAGCGTTTGCCAAGATTAATTGGTGTTGGTAGAGCG
AAAGAATTAATTTATACAGGAAGACGTATTTCGGCACAAGAAGCGAAAGAATATGGTCTAGTAGAATTTGTTGTACCAGT
TCATTTACTTGAAGAAAAAGCGATTGAAATAGCAGAGAAAATTGCTAGTAATGGTCCCATTGCTGTTCGATTAGCGAAAG
AAGCAATTTCAAACGGTATTCAAGTTGATTTACATACCGGATTACAAATGGAAAAACAAGCGTATGAAGGTGTAATCCAT
ACGAAAGACAGATTAGAAGGATTACAGGCATTCAAGGAAAAACGCACACCAATGTATAAGGGGGAGTAA

Upstream 100 bases:

>100_bases
TTATTAAGAGCGAGTCAATTTATTCAAAGTAAATTAAATATCCAATTACCGAGTCATGTGTATAGAGCGCTTCAACATAA
AACGATAAGTAGGTGAGAAG

Downstream 100 bases:

>100_bases
ATATGATAGACCAAAAACAACAATCGAATACATTTGAAGAACGAGTTGAAACGATTAAACGAGGCGGCGCACCAAAATAT
CATGAACAAAATAAAGCGAA

Product: enoyl-CoA hydratase

Products: crotonoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLQLQNISVDYATPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAGEKAFCAGADLKERAGMNEEQ
VRHAVSMIRTTMEMVEQLPQPVIAAINGIALGGGTELSLACDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRA
KELIYTGRRISAQEAKEYGLVEFVVPVHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
TKDRLEGLQAFKEKRTPMYKGE

Sequences:

>Translated_262_residues
MLQLQNISVDYATPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAGEKAFCAGADLKERAGMNEEQ
VRHAVSMIRTTMEMVEQLPQPVIAAINGIALGGGTELSLACDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRA
KELIYTGRRISAQEAKEYGLVEFVVPVHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
TKDRLEGLQAFKEKRTPMYKGE
>Mature_262_residues
MLQLQNISVDYATPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAGEKAFCAGADLKERAGMNEEQ
VRHAVSMIRTTMEMVEQLPQPVIAAINGIALGGGTELSLACDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRA
KELIYTGRRISAQEAKEYGLVEFVVPVHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
TKDRLEGLQAFKEKRTPMYKGE

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=49.21875, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=42.6229508196721, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=39.0438247011952, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=33.8645418326693, Blast_Score=121, Evalue=5e-28,
Organism=Homo sapiens, GI70995211, Length=264, Percent_Identity=29.5454545454545, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI68989263, Length=201, Percent_Identity=36.318407960199, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI20127408, Length=210, Percent_Identity=35.2380952380952, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI261878539, Length=115, Percent_Identity=44.3478260869565, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI37594471, Length=196, Percent_Identity=31.1224489795918, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI157694516, Length=220, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI213417737, Length=219, Percent_Identity=27.3972602739726, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=24.7899159663866, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=24.7899159663866, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221307494, Length=214, Percent_Identity=27.5700934579439, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI25453481, Length=242, Percent_Identity=24.7933884297521, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538573, Length=242, Percent_Identity=24.7933884297521, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI116235487, Length=177, Percent_Identity=27.1186440677966, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4757966, Length=235, Percent_Identity=25.1063829787234, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI51538581, Length=235, Percent_Identity=25.1063829787234, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI157694520, Length=185, Percent_Identity=29.7297297297297, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=24.7663551401869, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=24.7663551401869, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=24.7663551401869, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4757968, Length=242, Percent_Identity=24.3801652892562, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI49355787, Length=242, Percent_Identity=24.3801652892562, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI62530384, Length=232, Percent_Identity=28.8793103448276, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=40.7258064516129, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=35.2, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=30.7692307692308, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1788682, Length=199, Percent_Identity=39.6984924623116, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=33.4630350194552, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=28.6852589641434, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1790281, Length=193, Percent_Identity=32.6424870466321, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=217, Percent_Identity=47.4654377880184, Blast_Score=210, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=43.1451612903226, Blast_Score=197, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=39.5161290322581, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17560910, Length=241, Percent_Identity=31.1203319502075, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=227, Percent_Identity=30.8370044052863, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=275, Percent_Identity=26.1818181818182, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=225, Percent_Identity=32.8888888888889, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17535521, Length=206, Percent_Identity=31.5533980582524, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=239, Percent_Identity=25.5230125523013, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=253, Percent_Identity=31.6205533596838, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=253, Percent_Identity=31.6205533596838, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=253, Percent_Identity=31.6205533596838, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=190, Percent_Identity=31.0526315789474, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=190, Percent_Identity=31.0526315789474, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=230, Percent_Identity=26.9565217391304, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=251, Percent_Identity=27.4900398406374, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=177, Percent_Identity=26.5536723163842, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=50.3875968992248, Blast_Score=247, Evalue=5e-66,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=40.5737704918033, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=40.5737704918033, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=29.9559471365639, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24650670, Length=194, Percent_Identity=36.0824742268041, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21357171, Length=190, Percent_Identity=32.1052631578947, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=25.2032520325203, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19921000, Length=226, Percent_Identity=31.4159292035398, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583077, Length=226, Percent_Identity=31.4159292035398, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583079, Length=226, Percent_Identity=31.4159292035398, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28571729, Length=198, Percent_Identity=30.8080808080808, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571730, Length=198, Percent_Identity=30.8080808080808, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24654903, Length=173, Percent_Identity=23.6994219653179, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921018, Length=171, Percent_Identity=30.9941520467836, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24583165, Length=171, Percent_Identity=28.6549707602339, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 28463; Mature: 28463

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLQNISVDYATPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAG
CEEEECCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
EKAFCAGADLKERAGMNEEQVRHAVSMIRTTMEMVEQLPQPVIAAINGIALGGGTELSLA
CCEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEECCCCEEEEE
CDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRAKELIYTGRRISAQEAKEYGL
EEEEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCH
VEFVVPVHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCEEEEECCCHHHHHHHCCHHC
TKDRLEGLQAFKEKRTPMYKGE
CHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLQLQNISVDYATPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAG
CEEEECCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
EKAFCAGADLKERAGMNEEQVRHAVSMIRTTMEMVEQLPQPVIAAINGIALGGGTELSLA
CCEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEECCCCEEEEE
CDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRAKELIYTGRRISAQEAKEYGL
EEEEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCH
VEFVVPVHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCEEEEECCCHHHHHHHCCHHC
TKDRLEGLQAFKEKRTPMYKGE
CHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]