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The map label for this gene is rob [H]

Identifier: 227814605

GI number: 227814605

Start: 1832658

End: 1833752

Strand: Reverse

Name: rob [H]

Synonym: BAMEG_2015

Alternate gene names: 227814605

Gene position: 1833752-1832658 (Counterclockwise)

Preceding gene: 227814607

Following gene: 227814603

Centisome position: 35.06

GC content: 29.77

Gene sequence:

>1095_bases
ATGAATGAACATATACAGCAGATGATTGACTGGATTGAGGTCAATTTAAAAAAGGAATTTTCATTAGATGAATTGTCCCG
TTATATGGGGTATTCTCCTTATTATTGTTCTTTTAAATTTCACCAAGTAACGGGTTTCAGTATTAGACGCTATATTCTTC
TTAGAAGGTTATATTTATCTATAGAAGATTTAAAGAATGGTAGGAAAATAATTGAGATCGCATTGGATTATGATTACTCT
TCCCAAGAGGCTTATAGTAGAGCTTTCAAAAATGTTTTTGGAATGAATCCAAGAGAATACCAACTTAGCAAAATGCCTAT
TCAATCATTTGTTAAACTCAATTTAAATAATGAAGGGGCGTTTAATGTGAATATTTCTAGAAAAATAGAGGTTGAACAGT
TGCGAAATAGGAAGAGTGAGCTATTTGATAAAGAGGTACTTAACATATTAAATGGTCAACTTATGTATGAAGAATTTAAA
AACGAAAAGCTAATGGGTGATTCTGATTATGCACCATTTAATGAAGCGATGTGTGTAAACCGAGTTACTACACTAGTTTT
TGATGAAGAATTTATTAAGACAAGGGCAGCAGGACATAACAGTTCAGTAGAAAGTTACACAAATAAGGTTATAGATCCAT
TAAAGAAACTTTTTACGAAAGAGTATAAATGTATTGTTTTATGGTTTGGTGAAGATATGTTCTGCCAAATGAACTTACTT
ACCATACTTTCATACCTTGAACATTCTCGTTATGAGGGGAAGTTATACTTAAATAGCTTCAGAGAAGATGAATTTAAAGT
AAATCAAATTGAACTTGAATTAGGAAAATATTCTTCGGTATACAATGAAGTATTAGTAAATCATAAAAAGACTTTCTATA
AGGTACCACCTGTAATGTATCAGGCTATAGATTTATATTTAGAAATGCTAAAAGAAGATAATGCAGTAATAAAATTCATC
TCTAGAAATAAAGATTTATCAACTCGTGAATTATTAATAAAGTTGTTTCATCTTTTTCCAACAATTGGATATGGAGATAC
TCAGTACATAGAGCTGATTAATAAAATAAAGAAGAAAGCTACACCTAAAATATAG

Upstream 100 bases:

>100_bases
AATTTAAAGATATTACAGAAAGTCTCATGTTATAAAACACAAGAAATATCAAAAGAAAGAAATGCACTTCTCTTATACTT
TTAAATAAGGAGTGGAGGAA

Downstream 100 bases:

>100_bases
GTATAGTTTTCTTCTTATAATTGCTCAAGGAATTCTAATTCTTTTTCTACAAATAGCATTAATTCTTTAATTGTTTCGCC
AGAAAAATCAAAACGAATAC

Product: transcriptional regulator, AraC family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRLYLSIEDLKNGRKIIEIALDYDYS
SQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNNEGAFNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYEEFK
NEKLMGDSDYAPFNEAMCVNRVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQMNLL
TILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPPVMYQAIDLYLEMLKEDNAVIKFI
SRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIKKKATPKI

Sequences:

>Translated_364_residues
MNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRLYLSIEDLKNGRKIIEIALDYDYS
SQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNNEGAFNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYEEFK
NEKLMGDSDYAPFNEAMCVNRVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQMNLL
TILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPPVMYQAIDLYLEMLKEDNAVIKFI
SRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIKKKATPKI
>Mature_364_residues
MNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRLYLSIEDLKNGRKIIEIALDYDYS
SQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNNEGAFNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYEEFK
NEKLMGDSDYAPFNEAMCVNRVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQMNLL
TILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPPVMYQAIDLYLEMLKEDNAVIKFI
SRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIKKKATPKI

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081928, Length=106, Percent_Identity=33.9622641509434, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790497, Length=98, Percent_Identity=34.6938775510204, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 43240; Mature: 43240

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRLYLS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHHH
IEDLKNGRKIIEIALDYDYSSQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNNEGA
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHEEECCCCCE
FNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYEEFKNEKLMGDSDYAPFNEAMCVN
EEEEECCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHH
RVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQMNLL
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
TILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPPVMY
HHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCHHEEECCHHHH
QAIDLYLEMLKEDNAVIKFISRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIKKKA
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
TPKI
CCCC
>Mature Secondary Structure
MNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRLYLS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHHH
IEDLKNGRKIIEIALDYDYSSQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNNEGA
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHEEECCCCCE
FNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYEEFKNEKLMGDSDYAPFNEAMCVN
EEEEECCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHH
RVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQMNLL
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
TILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPPVMY
HHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCHHEEECCHHHH
QAIDLYLEMLKEDNAVIKFISRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIKKKA
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
TPKI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]