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The map label for this gene is pbpE [H]

Identifier: 227814533

GI number: 227814533

Start: 1766552

End: 1767574

Strand: Reverse

Name: pbpE [H]

Synonym: BAMEG_1941

Alternate gene names: 227814533

Gene position: 1767574-1766552 (Counterclockwise)

Preceding gene: 227814534

Following gene: 227814532

Centisome position: 33.8

GC content: 32.16

Gene sequence:

>1023_bases
ATGAAAACAAAAGATCAAATAGATAACATTGTAAAAGAAACATATCGTCATATAGATTTCTCTGGAGTAATTTTAGTGAA
AGAAAAAAAGGGCATAGTCTATGAAGAGGTGTTTGGTTATGCAAATCGAAATGAGTGTATAAACAATACGCTACAAACTA
GGTTTGGAATTGCCTCTGGGTGTAAAATATTCACAGCTATCGGTATCTGTCAACTTGTTGAAAATGGGGTAATTACTTTT
CAAACAAAGTTAAAAGAGTGTTTAAGTGTTAACTTTCCAAACTTTAATGAAGATATTACAATACATCACCTTTTGACGCA
TAGTTCCGGTATTCCAGATTATTTTGATGAAAGTATTATGGATAACTTCGAAGATCTTTGGAAACAAACACCTATGTATT
TATTAAAAAGTTTAAAAGACTTTTTACCATTATTTCAAAACAGAGATATGATGTATGCACCAGGAAGTAAGTTTCACTAC
AACAATGCAGGTTTTATCATACTGGGATTAATAATCGAAGAACAGACAGGACTTACATTTACGGAGTATATAGAAAAAAA
CATATTTAATCCAATTGGTATGAATCATTCGGGCTATTTTTCTTTAGATAGATTACCAAGGCAGACAGCTCTCGGATATA
TAAAAGATGAAATTAGCCAGACATGGAGAACAAATGCTTACTCTATTCCGATAAAAGGTGGTTCTGATGGTGGAGCTTTT
ATTACTGCGTCAGACATGCTGAAGTTTTGGGAAGCGTTATTTAACTATGAAATTATAAGCCAAGAATATACAAAAATACT
TTTAACTCCTCACATCCAAGTAAATAATAATCAATCTTATGGCTATGGAATATGGATTGAAACAAGAGAAAACAAGATAT
TTAAATATCATGTAATGGGATATGACCCAGGTGTCAGTTTTCGCTCGGCCGTATATCCAGACTTAGGAATTACATTAGTT
ATTCCGTCAAATAAAGGAGCAGGAGCCGAAAAATTAATGATAGAAATAGAAGGAAGTTTTTAA

Upstream 100 bases:

>100_bases
TTGATTTAAATCTTACGATGTTCAATAGGAGGGTTATCCCTCCTATATATGGAAATATAAGAAAGAAGAACAATATTGAT
AAACGAGGGTGATTTTTGTA

Downstream 100 bases:

>100_bases
TTATATGGATATAGAAATTAGAGCGTATAGAAAAGAGGATGAAACCGGTTGGGTACGTTGCCGTGTGTTATCTTTTTTAG
ATACAGCTTATTACGATAAT

Product: penicillin-binding protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEVFGYANRNECINNTLQTRFGIASGCKIFTAIGICQLVENGVITF
QTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHY
NNAGFIILGLIIEEQTGLTFTEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVYPDLGITLV
IPSNKGAGAEKLMIEIEGSF

Sequences:

>Translated_340_residues
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEVFGYANRNECINNTLQTRFGIASGCKIFTAIGICQLVENGVITF
QTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHY
NNAGFIILGLIIEEQTGLTFTEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVYPDLGITLV
IPSNKGAGAEKLMIEIEGSF
>Mature_340_residues
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEVFGYANRNECINNTLQTRFGIASGCKIFTAIGICQLVENGVITF
QTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHY
NNAGFIILGLIIEEQTGLTFTEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVYPDLGITLV
IPSNKGAGAEKLMIEIEGSF

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115533026, Length=272, Percent_Identity=26.4705882352941, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17550934, Length=180, Percent_Identity=28.3333333333333, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 38853; Mature: 38853

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEVFGYANRNECINNTLQTRFGIASG
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCC
CKIFTAIGICQLVENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIM
HHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
DNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHYNNAGFIILGLIIEEQTGLTF
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCEEEEEEEEECCCCCHH
TEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
HHHHHHHCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCE
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMG
EEHHHHHHHHHHHHCHHHHCCCCEEEEEECEEEECCCCCCEEEEEEEECCCEEEEEEEEE
YDPGVSFRSAVYPDLGITLVIPSNKGAGAEKLMIEIEGSF
ECCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEVFGYANRNECINNTLQTRFGIASG
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCC
CKIFTAIGICQLVENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIM
HHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
DNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHYNNAGFIILGLIIEEQTGLTF
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCEEEEEEEEECCCCCHH
TEYIEKNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
HHHHHHHCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCE
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMG
EEHHHHHHHHHHHHCHHHHCCCCEEEEEECEEEECCCCCCEEEEEEEECCCEEEEEEEEE
YDPGVSFRSAVYPDLGITLVIPSNKGAGAEKLMIEIEGSF
ECCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]