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The map label for this gene is rihA [H]

Identifier: 227813620

GI number: 227813620

Start: 922441

End: 923382

Strand: Direct

Name: rihA [H]

Synonym: BAMEG_1024

Alternate gene names: 227813620

Gene position: 922441-923382 (Clockwise)

Preceding gene: 227813619

Following gene: 227813621

Centisome position: 17.64

GC content: 36.52

Gene sequence:

>942_bases
TTGAGGGCGATGAAAAAAGTATTATTTTTAGGAGACCCAGGAATTGATGACTCTTTAGCAATTATGTATGGATTGTTGCA
TCCTGATATTGATATTGTTGGTGTAGTAACTGGATATGGAAATGTAACGCAAGAAAAGGCGACAAGTAATGCGGCATATT
TATTGCAACTGGCAGGACGGGAAGATATACCTATTATTAATGGTGCGAAAATCCCTTTATCTGGAGATATTACAACGTAT
TATCCAGAAATTCATGGGGCGGAAGGCTTAGGACCAATTCGACCGCCGAAAAATCTTTCTCCAAATATAAGGCCTTTTTG
TGAGTTTTTTGACATTCTTGAAAAATATAAAGGAGAATTAATTATAGTTGATGCTGGGAGGTCAACGACACTTGCAACAG
CATTTATTTTAGAAAAACCATTGATGAAGTATGTGAAAGAATATTATATAATGGGCGGTGCTTTTTTAATGCCTGGAAAT
GTTACACCAGTCGCAGAAGCGAATTTTCATGGTGACCCTATTGCATCACAATTAGTCATGCAAAATGCCAAGAATGTGAC
GTTGGTGCCGCTGAATGTTACATCTGAAGCTATAATCACGCCAGAGATGGTAAAGTACATTACGAAACATTCTAAAACGA
GTTTTAATAAATTAATTGAACCGATTTTTACGTATTATTATAAAGCTTATAGAAAGTTAAATCCGAAAATAACAGGAAGT
CCAGTACATGACGTTGTTACAATGATGGTCGCGGCGAATCCTTCAATACTGGATTATGTGTATCGTCGTGTAGATGTAGA
TACAGTGGGGATTGCAAAAGGAGAAAGTATTGCAGATTTCCGTCCTCAACCTGATGCAAAAGCCTTAAAAAATTGGGTAC
GAATTGGTTGGTCATTACATTATAAAAAATTCCTTGAGGATTTTGTGAAAATCATGACGTAG

Upstream 100 bases:

>100_bases
ACGTAGTCCTTACAGGACTGCGTTTTTTCGTATAAATTTGTTCTTTTTATTCTGTAATCCTGTGAATATTCATAAATTAA
TAACAATTGAGGGGATGGAG

Downstream 100 bases:

>100_bases
ACATAATGGGAGTATACAAGTTAAAATAATGGAGATGGTTATTCAAATAACCATCTCCATTATTTTGTTTTTTTAAGATA
GAGAGAAAGAGTTACACAAT

Product: inosine-uridine preferring nucleoside hydrolase family protein

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTY
YPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGN
VTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGS
PVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT

Sequences:

>Translated_313_residues
MRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTY
YPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGN
VTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGS
PVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT
>Mature_313_residues
MRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTY
YPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGN
VTPVAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGS
PVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=280, Percent_Identity=29.2857142857143, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788486, Length=279, Percent_Identity=27.2401433691756, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786213, Length=306, Percent_Identity=28.4313725490196, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI32565474, Length=202, Percent_Identity=28.7128712871287, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565698, Length=200, Percent_Identity=29, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320608, Length=275, Percent_Identity=30.5454545454545, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34823; Mature: 34823

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGR
CCCHHCEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECCC
EDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGEL
CCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
IIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVM
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHH
QNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGS
HCCCCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLH
HHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
YKKFLEDFVKIMT
HHHHHHHHHHHCC
>Mature Secondary Structure
MRAMKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGR
CCCHHCEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECCC
EDIPIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGEL
CCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
IIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVM
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHH
QNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGS
HCCCCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PVHDVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLH
HHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
YKKFLEDFVKIMT
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA