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The map label for this gene is dctA [H]

Identifier: 227813583

GI number: 227813583

Start: 883056

End: 884318

Strand: Direct

Name: dctA [H]

Synonym: BAMEG_0987

Alternate gene names: 227813583

Gene position: 883056-884318 (Clockwise)

Preceding gene: 227813582

Following gene: 227813584

Centisome position: 16.88

GC content: 35.31

Gene sequence:

>1263_bases
ATGAGAATTGTAAAGAATTTAACATTCCAAGTTATTGTGGCCATTATTTGTGGTATTGCAGTAGGAGCAATTTGGCCGAG
TGTTGGACAAGAGATGAAGCCAATTGGTGAAACATTCATCAATATGATTAAAATGGTTATTGCACCCATTATCTTTTTAA
CAATTGTGCTTGGTATTGCAAGTATGGGAAGTATGAAAAAGGTAGGCCGTGTTGGCGGGAAGGCACTATTATATTTTGAA
ATTGTTACAACAGCGGCTTTAATAATTGGTATCATTGTAGCGAATGTTGTACGTCCAGGAGATGGATTAGATCCCTCAAA
ACTAAAAGGCGGAGATGTTTCACAATATGTACAAAGCGGGCAAGAAATGAAATGGATGGATTTCTTTTTACATATTGTGC
CGTCTAACATGTTTGAAGCATTTGCAAAAGGTGATATTTTACAAGTTTTGTTCTTCTCCATTTTATTCGGTGCAGGGTTA
ACGATGTTAGGGAAGAATGGACAACCGGTAATCGACTTTTTTGAAAGATTATCGAAAGTATTTTTTAACATTTTATCAAT
TGTAATGAAATTAGCACCGGTTGGAGCATTCGGCGGTATGGCGTTTACAATTGGCAAATATGGTTTAAGTACACTTATTC
CACTTGGTAAATTAATGATTTGTGTATATGCAACAATGGCATTATTTGTTTTTATTGTTTTAAATTTCATTTGTAAATTT
TACAAATTTAGTTTGTGGAAATATTTAGCGCATATTAAAGAAGAATTACTTATTGTTCTTGGGACATCATCATCAGAATC
AGTATTGCCGCGAATGATGACAAAGATGGAAGACTTCGGATGTTCGAAGCCGGTAGTAGGTTTAGTCATTCCAACGGGGT
ACTCTTTTAATTTAGATGGAACAACAATTTATTTATCGATGGCTACTATATTTTTAGCGCAAGTCTTTCACGTCGATCTT
TCACTAGGTCAACAATTAACTATAATAGCTATTTTATTAGTTACATCTAAAGGAGCAGCGGCAGTAACAGGCGGGGGATT
TATTGTATTAGCCTCTACTTTGTCAGCAATGAATGTTATCCCACTAGAAGGGTTAGCGCTATTACTTGGTGTAGATCGTT
TCATGTCAGAAGCACGTGCTATCGTTAATTTAATTGGTAATGGCATTGCGACTGTAGTTGTTGCAAAAAGTGAAAATGAA
TTTGATAACGAGAAGTATATGAAAGTAGTAGAGGAAATGAAAAGAGAAAAAATGGCCGGGTAA

Upstream 100 bases:

>100_bases
AGTTAAGGTTTAAGTTACATAACAGGGGGGCCATCACATAGAATAAATGTTATTGATGAAAATAGTTTGGGAGAAATGAA
AAAATAAGGGAGTATTAAAA

Downstream 100 bases:

>100_bases
AAAGTCAGCTTTGCTGACTTTTTTATTTTTGTAGAAAAGAGGTTTGTTACGATATGCATTATTTATTAACAATAATTTAC
AAGTGTTTTATAGTGAAGGG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKF
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDNEKYMKVVEEMKREKMAG

Sequences:

>Translated_420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKF
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDNEKYMKVVEEMKREKMAG
>Mature_420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKF
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDNEKYMKVVEEMKREKMAG

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=449, Percent_Identity=24.7216035634744, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI21314632, Length=431, Percent_Identity=25.754060324826, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI40254478, Length=475, Percent_Identity=24.4210526315789, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI5032093, Length=423, Percent_Identity=26.0047281323877, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI223468566, Length=285, Percent_Identity=28.4210526315789, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI223468564, Length=285, Percent_Identity=28.4210526315789, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI66773030, Length=421, Percent_Identity=26.1282660332542, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI194239697, Length=432, Percent_Identity=24.537037037037, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI4827012, Length=270, Percent_Identity=27.4074074074074, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=24.2021276595745, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI301601644, Length=147, Percent_Identity=29.9319727891156, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789947, Length=410, Percent_Identity=55.609756097561, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1790514, Length=430, Percent_Identity=40.2325581395349, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1789473, Length=319, Percent_Identity=22.884012539185, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=425, Percent_Identity=26.8235294117647, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71983099, Length=424, Percent_Identity=25.2358490566038, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=424, Percent_Identity=25.2358490566038, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=406, Percent_Identity=26.8472906403941, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=413, Percent_Identity=25.6658595641646, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=437, Percent_Identity=24.9427917620137, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=436, Percent_Identity=23.394495412844, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24583025, Length=407, Percent_Identity=23.0958230958231, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17137668, Length=407, Percent_Identity=23.0958230958231, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583023, Length=407, Percent_Identity=23.0958230958231, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281360483, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137666, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281360481, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45530; Mature: 45530

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure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PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]