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The map label for this gene is rlmN [H]

Identifier: 227813228

GI number: 227813228

Start: 557328

End: 558416

Strand: Direct

Name: rlmN [H]

Synonym: BAMEG_0629

Alternate gene names: 227813228

Gene position: 557328-558416 (Clockwise)

Preceding gene: 227813227

Following gene: 227813229

Centisome position: 10.66

GC content: 36.64

Gene sequence:

>1089_bases
GTGGAAACGACTGTGAGAAAACAAAAGAAAAACTTAGAAACGAAAAAACCATCAATTTATTCTTTACAACTTCATGAAAT
GCAAGATTGGTTAAAGGAGCAAGGAGAACCAAAGTTCCGTGCTGGACAAATTTTTGATTGGTTATATAAAAAACGTGTAA
AAAATTATGAGGATATGTCAAACCTTTCTAAAGGATTGCGTGAAAAATTGTCGAATTCCTTTGATATTACTACTTTACAT
ACGTTAGTAAAACAAACGTCTTCGGATGGAACAATTAAATTCTTATTCCAATTATATGATGGATATTCTATTGAAACGGT
ATTAATGCGACATGAATATGGAAATTCCATTTGTGTAACAACACAAGTAGGTTGTCGTATTGGGTGTACGTTCTGTGCTT
CAACGCTTGGTGGTTTAAAGCGAAACTTAGAAGCTGGAGAAATTGTAGCGCAAGTAGTAGAAGTGCAACGTGCACTTGAT
GAATCAGAAGAACGTGTAAGTTCTCTTGTCGTTATGGGAATTGGAGAACCATTTGATAACTACGATAATTTAATGGGATT
CTTACGCATCATTAACCATGAAAAAGGACTTCATATTGGCGCGAGACATATGACAGTTTCGACAAGTGGAATCATTCCAA
AAATTTATAAGTTCGCTGAAGAAGACTTACAAATTAATTTTGCGATTTCACTGCATGCTCCAAACTCAGAATTACGTTCA
AAATTAATGCCGATTAACCGTGCTTATAAGCTACCTGATTTAATGGAAGCTATTAAATACTATGTAAATAGAACAGGGCG
TCGTATTACTTTTGAATATGGACTATTTGGAGGAGAAAATGACCAAGTTGAGCACGCTGAAGAACTTGCTGCGCTCTTAA
AAGGTGTAAAATGTCATGTGAACTTAATTCCGGTAAACTACGTACCTGAACGTGATTATGTACGTACGCCACGTGAACAA
ATTTTCCTATTTGAAAAAACGTTGAAAGATCGTGGAGTAAATGTAACGATTCGCCGTGAACAAGGTCATGATATTGATGC
AGCCTGCGGTCAGTTGCGTGCGAAGGAGCGCAAAGAAGAGACGAGGTGA

Upstream 100 bases:

>100_bases
CTAAGTCCGTATATGGATGAAGGTCAAGTACAAATTTTACCGCATTATTTTGCAACAGATGGCTTTTATATTGCTTGTTT
AAGGAAGAAGGTGTAGCATC

Downstream 100 bases:

>100_bases
CGAGATGAAAGCCGTGTTTCTATCAGATAAAGGAAAAGTTCGTCAACATAATGAAGATAGTGCAGGAGTTTTTCACAATT
TAGATGGAAATATTTTAGCG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFDITTLH
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEETR

Sequences:

>Translated_362_residues
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFDITTLH
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEETR
>Mature_362_residues
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFDITTLH
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=352, Percent_Identity=35.7954545454545, Blast_Score=242, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41576; Mature: 41576

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMS
CCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
NLSKGLREKLSNSFDITTLHTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVT
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEE
TQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALDESEERVSSLVVMGIGEPFDN
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHEEEEECCCCCCC
YDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
HHHHHHHHHHHHCCCCCEECCEEEEEECCCCHHHHHHHHHCCEEEEEEEEEECCCHHHHH
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHV
HHCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEE
NLIPVNYVPERDYVRTPREQIFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEE
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
TR
CC
>Mature Secondary Structure
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMS
CCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
NLSKGLREKLSNSFDITTLHTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVT
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEE
TQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALDESEERVSSLVVMGIGEPFDN
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHEEEEECCCCCCC
YDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
HHHHHHHHHHHHCCCCCEECCEEEEEECCCCHHHHHHHHHCCEEEEEEEEEECCCHHHHH
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHV
HHCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEE
NLIPVNYVPERDYVRTPREQIFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEE
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA