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The map label for this gene is vanS [H]

Identifier: 227812938

GI number: 227812938

Start: 265387

End: 266844

Strand: Direct

Name: vanS [H]

Synonym: BAMEG_0318

Alternate gene names: 227812938

Gene position: 265387-266844 (Clockwise)

Preceding gene: 227812937

Following gene: 227812943

Centisome position: 5.07

GC content: 34.71

Gene sequence:

>1458_bases
TTGATTGAGAAATTAGTAAAGGGCGTACGAAAGAGTATAAGAATTCAACTGATTACTACTTTTGCCGCTTGTGCGTTATT
AGGAGTTTTTTTTGCAAAGGGAGCTGCACCATTTTTTGAGAATGCGAATCGTCAAGCGACTATTGATTATCGAGCTGGTA
TGGAACAAATTAATCACGAAGCTCAAAGAGCAGCAAATAGCTCGGTTCATGAAAATAAATTAGAAGCTATATCCAATATG
ATCGAAATGGAGAATCAAAATTTAGAGCGAGGATCTAGAGCTCTAAAAATATTGGTTACTGATGAAAGTGGTAAAGTTTT
ATATAAAACGAAGCAGGCGCAAGAAGAGCAAATCGATTTGCATAATACGATTCGTAATGCAGCATCATTTGCGATTAACT
ATTCAAATGGTCGAGATGTATTTGAAAATACAAGAAAAGAATTTATAGCTTTTTCGCCTATTACAATAGAAGATAAGAAC
TTATATATGTTCGTTAGTGGAATACCAGATGGTGTAGTGATGTATGATACACAAGAAGGACCATTTCCATTTTTAATTGG
TGTACTTGTATTTATTTTCTCTTTCTTCTATATAACAAAGAGAAAGATGAAGCAAATTGAAGCGATGGCAGAAGGTGTAA
AGGAAATAGAAAAAGGCAACTTAGCGTACCGTATAGAGAAGAAAGGTGAAGATGAGATTGCATCTTTAACAGAAAATATT
AATAATATGGCAGAAGAGCTTATGAATAATATAGAAAAGGAACGTAAGTTAGAGAAGCAAAAGAACGAGCTTATTACAAA
TGTATCTCACGATTTGCGTACACCACTGACTTCTATTATGGGTTACTTGCGATTACTTCGAGATTCTAAATATGAAAATA
AAGAGCAACACGATGAATATACGAGAATTGCTTTTGCGAAGTCAGAGCAGTTAAAGAATTTAATAGAAGATTTATTTGAG
TATACGAAGTTAACGAATGAGCAAGTTGTATTAGAAAAACAAGAAGTATGTGTAAATGAGTTATTGGAGCAATTAATAGA
AGAGTTAGTACCGCAAGCGGAAGAGCATGGACTTACATTTGTTAAGAAGTTTCCTGAGGAACGTGCCTATGCAGCGATTG
ATTCGGAAAAGATGGTCCGTGTATTTGATAATCTATTAATGAACGCGATTAAGTATAGTAAAGATGATGGGGAGATAAAA
GTTTCCCTTCAAAGGCAGCGCTGGGAGATACAAATTGTAATTGCGAATCATAGTGAAGAGTTTACGAGAGAAGAGTTAGC
GAGTTTGTTTGAACGTTTTTATAAGAAAGATCAATCTAGAAGTAGAGTAACAGAAGGGTCAGGCCTTGGATTGGCGATTG
CGAAAAGTATTGTTGAATTACAAGGTGGTAGTATTCGAGCTGAATGTAAGGATGGTATTATTCAGTTTATCGTCTCGTTA
CCAATTATAGAAAGATAA

Upstream 100 bases:

>100_bases
AATCCAAGGAAACCTAGATATATAAAAACAGTATGGGGAGTGGGGTATAAGATTGAAAAAGATATTTAATCCATTTACTT
ATATAAGGAAAGCAAGACAA

Downstream 100 bases:

>100_bases
TAAACGAATAGAATGGTATATGATAAAAAGGCTTATTTTAAAAGGGATAAGCCTTTTTATATTTTTTTAGAAAAACATGT
TGACGATTAGGTTATAGAGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MIEKLVKGVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANRQATIDYRAGMEQINHEAQRAANSSVHENKLEAISNM
IEMENQNLERGSRALKILVTDESGKVLYKTKQAQEEQIDLHNTIRNAASFAINYSNGRDVFENTRKEFIAFSPITIEDKN
LYMFVSGIPDGVVMYDTQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENI
NNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFE
YTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIK
VSLQRQRWEIQIVIANHSEEFTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGSIRAECKDGIIQFIVSL
PIIER

Sequences:

>Translated_485_residues
MIEKLVKGVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANRQATIDYRAGMEQINHEAQRAANSSVHENKLEAISNM
IEMENQNLERGSRALKILVTDESGKVLYKTKQAQEEQIDLHNTIRNAASFAINYSNGRDVFENTRKEFIAFSPITIEDKN
LYMFVSGIPDGVVMYDTQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENI
NNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFE
YTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIK
VSLQRQRWEIQIVIANHSEEFTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGSIRAECKDGIIQFIVSL
PIIER
>Mature_485_residues
MIEKLVKGVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANRQATIDYRAGMEQINHEAQRAANSSVHENKLEAISNM
IEMENQNLERGSRALKILVTDESGKVLYKTKQAQEEQIDLHNTIRNAASFAINYSNGRDVFENTRKEFIAFSPITIEDKN
LYMFVSGIPDGVVMYDTQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENI
NNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFE
YTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIK
VSLQRQRWEIQIVIANHSEEFTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGSIRAECKDGIIQFIVSL
PIIER

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=25.9786476868327, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786783, Length=308, Percent_Identity=25.6493506493506, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788713, Length=249, Percent_Identity=28.1124497991968, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1790346, Length=284, Percent_Identity=25.3521126760563, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787894, Length=346, Percent_Identity=26.878612716763, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789149, Length=320, Percent_Identity=25.9375, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=27.5303643724696, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1786912, Length=281, Percent_Identity=25.2669039145907, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082128, Length=290, Percent_Identity=28.9655172413793, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786600, Length=210, Percent_Identity=27.6190476190476, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788549, Length=287, Percent_Identity=24.0418118466899, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994928, Length=217, Percent_Identity=25.3456221198157, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=26.431718061674, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788279, Length=254, Percent_Identity=25.9842519685039, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789808, Length=280, Percent_Identity=26.4285714285714, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=398, Percent_Identity=23.1155778894472, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI87081816, Length=233, Percent_Identity=22.7467811158798, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55703; Mature: 55703

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKLVKGVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANRQATIDYRAGMEQINHE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEHHHHHHHHHHHH
AQRAANSSVHENKLEAISNMIEMENQNLERGSRALKILVTDESGKVLYKTKQAQEEQIDL
HHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCEEEEEEECCCCCEEEECHHHHHHHHHH
HNTIRNAASFAINYSNGRDVFENTRKEFIAFSPITIEDKNLYMFVSGIPDGVVMYDTQEG
HHHHHHHHHEEEECCCCCHHHHHHHHHHEEECCEEEECCCEEEEEECCCCCEEEEECCCC
PFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
NNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
TRIAFAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHH
VKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRWEIQIVIANHSEE
HHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEEECCCHH
FTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGSIRAECKDGIIQFIVSL
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHC
PIIER
CCCCC
>Mature Secondary Structure
MIEKLVKGVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANRQATIDYRAGMEQINHE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEHHHHHHHHHHHH
AQRAANSSVHENKLEAISNMIEMENQNLERGSRALKILVTDESGKVLYKTKQAQEEQIDL
HHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCEEEEEEECCCCCEEEECHHHHHHHHHH
HNTIRNAASFAINYSNGRDVFENTRKEFIAFSPITIEDKNLYMFVSGIPDGVVMYDTQEG
HHHHHHHHHEEEECCCCCHHHHHHHHHHEEECCEEEECCCEEEEEECCCCCEEEEECCCC
PFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
NNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
TRIAFAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHH
VKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRWEIQIVIANHSEE
HHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEEECCCHH
FTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGSIRAECKDGIIQFIVSL
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHC
PIIER
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]