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The map label for this gene is mltA [H]

Identifier: 227082436

GI number: 227082436

Start: 2399900

End: 2401006

Strand: Reverse

Name: mltA [H]

Synonym: VCM66_2235

Alternate gene names: 227082436

Gene position: 2401006-2399900 (Counterclockwise)

Preceding gene: 227082438

Following gene: 227082435

Centisome position: 83.01

GC content: 49.5

Gene sequence:

>1107_bases
GTGAGTAAACGTCTTCTTTCTCTCGCCAGTTTGGCACTGCTGTTTGGTTGTGCTCAGCAACCTAACGATCGTACTCAGCA
ATACCAACAACAAACTTTTCCACACATTTTGAATCGTGCCGATGTGGTTGAATCCAATAAGCCGCGTGACTATACCGAAT
TCAGTAAACAGAGTGAGTTGGTCGTGCAAGGCTCAGCCTCCATGGCCAAGATCTATCGACCGCTGTATGAACAATTGAAT
GAGTGGGTTCTGCAAAGCGGAGATCCTGCCACTTTAGCGCAATTTGGCATTCAAGCAGCGCAGTTAGGTGGCGGGGATAA
ACAAGGTAATGTATTGTTTACTGGATATTTTTCCCCTGTGATTGAGCTGCGTCATCAACCCGACAGCGTGTTTAAATATC
CGGTTTACGGCTTGCCAAAATGCAACAAAAACTGCCCCACTCGAGCGGAGATTTACCAAGGTGCGCTGGATGGTCAAGGT
TTAGAATTGGGCTATGCGGAGAACTTGATCGATCCTTTCATCATGGAAGTGCAGGGCAGTGGCTTTGTTCATTTTGGGGA
TGACGATACTTTGGAATACTTCGCTTACGCGGGTAAAAATAACAAGGCTTACGTCAGCATTGGTAAAGTGTTGATTGAAC
GCGGTTTGGTTGAGCGCGAGAAAATGTCGCTCAAAGCGATCAAAGATTGGGTGCTGGCCAACGATGAAGCAACCGTACGT
GAACTGCTTGAGGAAAACCCTTCCTTCGTATTTTTCAAACCCAGCGCCGCGGCACCTGTGAAGGGCAGCGCCGGCATTCC
TTTATTGCCGATGGCATCGGTTGCTGGGGACCGTTCAATTTTGCCTATGGGGACGCCAATTCTAGCGGAAGTCCCCCTGC
TGAATGCCGATGGCACTTGGTCTGGCGCACATCAACTGCGTTTGTTGATTGTGCTTGATACAGGCGGTGCCGTGAAACAA
AACCATCTCGACTTGTACCACGGTATGGGACCACGTGCAGGGTTAGAAGCGGGGCACTATAAGCACTTTGGCCGTGTGTG
GAAACTCGGGTTAGAAAACTCGCCTACCCAAGCACCATGGGCATTGCCGCCGGAAAAACAGCAATAG

Upstream 100 bases:

>100_bases
TCAGAAAAAGTAAAAAATAATCGCTATTAATCAAACGCCAATTGCACAGTTTCACTGACTTTGTCATGCTCTTGGCAAAC
ATAAACCATGGACCTCTTTT

Downstream 100 bases:

>100_bases
TTTTTCCTGATATTGTGCCGGTAGCAAACTGGACATTTTTGTGAAGAGTGCGTATAAATCGCACTCTTTATTTTTCAGCT
AGAGTGGAAATGTGGTTATG

Product: murein transglycosylase A

Products: Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase A [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSKRLLSLASLALLFGCAQQPNDRTQQYQQQTFPHILNRADVVESNKPRDYTEFSKQSELVVQGSASMAKIYRPLYEQLN
EWVLQSGDPATLAQFGIQAAQLGGGDKQGNVLFTGYFSPVIELRHQPDSVFKYPVYGLPKCNKNCPTRAEIYQGALDGQG
LELGYAENLIDPFIMEVQGSGFVHFGDDDTLEYFAYAGKNNKAYVSIGKVLIERGLVEREKMSLKAIKDWVLANDEATVR
ELLEENPSFVFFKPSAAAPVKGSAGIPLLPMASVAGDRSILPMGTPILAEVPLLNADGTWSGAHQLRLLIVLDTGGAVKQ
NHLDLYHGMGPRAGLEAGHYKHFGRVWKLGLENSPTQAPWALPPEKQQ

Sequences:

>Translated_368_residues
MSKRLLSLASLALLFGCAQQPNDRTQQYQQQTFPHILNRADVVESNKPRDYTEFSKQSELVVQGSASMAKIYRPLYEQLN
EWVLQSGDPATLAQFGIQAAQLGGGDKQGNVLFTGYFSPVIELRHQPDSVFKYPVYGLPKCNKNCPTRAEIYQGALDGQG
LELGYAENLIDPFIMEVQGSGFVHFGDDDTLEYFAYAGKNNKAYVSIGKVLIERGLVEREKMSLKAIKDWVLANDEATVR
ELLEENPSFVFFKPSAAAPVKGSAGIPLLPMASVAGDRSILPMGTPILAEVPLLNADGTWSGAHQLRLLIVLDTGGAVKQ
NHLDLYHGMGPRAGLEAGHYKHFGRVWKLGLENSPTQAPWALPPEKQQ
>Mature_367_residues
SKRLLSLASLALLFGCAQQPNDRTQQYQQQTFPHILNRADVVESNKPRDYTEFSKQSELVVQGSASMAKIYRPLYEQLNE
WVLQSGDPATLAQFGIQAAQLGGGDKQGNVLFTGYFSPVIELRHQPDSVFKYPVYGLPKCNKNCPTRAEIYQGALDGQGL
ELGYAENLIDPFIMEVQGSGFVHFGDDDTLEYFAYAGKNNKAYVSIGKVLIERGLVEREKMSLKAIKDWVLANDEATVRE
LLEENPSFVFFKPSAAAPVKGSAGIPLLPMASVAGDRSILPMGTPILAEVPLLNADGTWSGAHQLRLLIVLDTGGAVKQN
HLDLYHGMGPRAGLEAGHYKHFGRVWKLGLENSPTQAPWALPPEKQQ

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi [H]

COG id: COG2821

COG function: function code M; Membrane-bound lytic murein transglycosylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789179, Length=350, Percent_Identity=48.2857142857143, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010611
- InterPro:   IPR014733
- InterPro:   IPR005300 [H]

Pfam domain/function: PF06725 3D; PF03562 MltA [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40489; Mature: 40357

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLLSLASLALLFGCAQQPNDRTQQYQQQTFPHILNRADVVESNKPRDYTEFSKQSEL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCE
VVQGSASMAKIYRPLYEQLNEWVLQSGDPATLAQFGIQAAQLGGGDKQGNVLFTGYFSPV
EEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCEEEEECHHHH
IELRHQPDSVFKYPVYGLPKCNKNCPTRAEIYQGALDGQGLELGYAENLIDPFIMEVQGS
HHHCCCCCCEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCEEEEECCC
GFVHFGDDDTLEYFAYAGKNNKAYVSIGKVLIERGLVEREKMSLKAIKDWVLANDEATVR
CEEEECCCCCEEEEEEECCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHH
ELLEENPSFVFFKPSAAAPVKGSAGIPLLPMASVAGDRSILPMGTPILAEVPLLNADGTW
HHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHEECCEECCCCCC
SGAHQLRLLIVLDTGGAVKQNHLDLYHGMGPRAGLEAGHYKHFGRVWKLGLENSPTQAPW
CCCCEEEEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCC
ALPPEKQQ
CCCCCCCC
>Mature Secondary Structure 
SKRLLSLASLALLFGCAQQPNDRTQQYQQQTFPHILNRADVVESNKPRDYTEFSKQSEL
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCE
VVQGSASMAKIYRPLYEQLNEWVLQSGDPATLAQFGIQAAQLGGGDKQGNVLFTGYFSPV
EEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCEEEEECHHHH
IELRHQPDSVFKYPVYGLPKCNKNCPTRAEIYQGALDGQGLELGYAENLIDPFIMEVQGS
HHHCCCCCCEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCEEEEECCC
GFVHFGDDDTLEYFAYAGKNNKAYVSIGKVLIERGLVEREKMSLKAIKDWVLANDEATVR
CEEEECCCCCEEEEEEECCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHH
ELLEENPSFVFFKPSAAAPVKGSAGIPLLPMASVAGDRSILPMGTPILAEVPLLNADGTW
HHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHEECCEECCCCCC
SGAHQLRLLIVLDTGGAVKQNHLDLYHGMGPRAGLEAGHYKHFGRVWKLGLENSPTQAPW
CCCCEEEEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCC
ALPPEKQQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]