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The map label for this gene is nqrB [H]

Identifier: 227082418

GI number: 227082418

Start: 2385192

End: 2386475

Strand: Reverse

Name: nqrB [H]

Synonym: VCM66_2217

Alternate gene names: 227082418

Gene position: 2386475-2385192 (Counterclockwise)

Preceding gene: 227082419

Following gene: 227082417

Centisome position: 82.5

GC content: 49.69

Gene sequence:

>1284_bases
GTGCTTAGACAAGATCGAGAAAGAAGGGTAATTTCCATGGGCCTTAAAAAGTTTCTTGAAGACATCGAGCATCATTTTGA
GCCAGGCGGCAAACACGAGAAGTGGTTTGCCCTGTATGAAGCAGCGGCGACGCTGTTCTATACACCAGGTCTGGTAACCA
AAAGAAGCTCGCACGTTCGTGATAGCGTTGACCTAAAACGTATCATGATCATGGTTTGGCTTGCCGTATTCCCAGCGATG
TTCTGGGGTATGTACAACGCTGGCGGCCAAGCTATTGCAGCACTTAACCATCTGTATTCAGGTGATCAACTCGCGGCGAT
TGTGGCGGGTAACTGGCACTACTGGCTAACCGAAATGCTTGGCGGAACCATGTCAAGCGATGCTGGTTGGGGCAGTAAAA
TGCTGTTGGGTGCAACCTACTTCCTACCTATCTACGCTACGGTGTTCATCGTCGGTGGTTTCTGGGAAGTGTTGTTCTGT
ATGGTGCGCAAGCATGAAGTCAACGAAGGTTTCTTCGTTACCTCTATTCTGTTTGCTCTGATTGTTCCGCCAACTCTGCC
TCTGTGGCAAGCTGCACTGGGTATTACCTTTGGTGTTGTGGTTGCCAAAGAAGTGTTTGGTGGTACAGGCCGTAACTTCC
TTAACCCAGCGCTGGCTGGCCGTGCTTTCCTGTTCTTTGCTTACCCAGCACAGATCTCAGGTGACCTAGTATGGACTGCG
GCAGATGGCTACTCAGGCGCAACTGCGCTGAGCCAATGGGCACAAGGTGGTGCAGGTGCACTGATTAACAACGCGACTGG
TCAAACGATCACTTGGATGGATGCTTTCATCGGTAACATCCCAGGTTCAATTGGTGAAGTGTCTACTCTGGCACTCATGA
TTGGTGCAGCGTTCATTGTTTATATGGGCATTGCTTCATGGCGCATTATTGGCGGCGTGATGATTGGTATGATCCTGCTG
TCGACACTGTTTAACGTGATTGGCTCAGATACCAACGCAATGTTCAACATGCCTTGGCATTGGCACCTAGTATTGGGTGG
TTTCGCATTCGGTATGTTCTTCATGGCGACTGACCCAGTTTCTGCGTCCTTCACCAATAGCGGTAAATGGGCTTACGGTA
TTCTGATCGGTGTTATGTGTGTGCTGATCCGTGTTGTGAACCCGGCTTACCCAGAAGGCATGATGCTGGCGATCCTATTC
GCGAACCTATTTGCGCCACTGTTTGACCATGTGGTTGTAGAGAGAAATATCAAGCGGAGATTAGCGCGCTATGGCAAGCA
ATAA

Upstream 100 bases:

>100_bases
GCGCGGTTCGCTTAGGTGCTTTAGAGCTGGATGAAGAAGATCTAGCACTGTGTACGTTCGTTTGCCCAGGCAAATACGAG
TACGGTCAGCTCCTGCGTGA

Downstream 100 bases:

>100_bases
CGATAGCATTAAAAAGACGCTGTTTGTTGTTATCGCGTTGAGCCTAGTGTGCTCAATCATTGTATCGGCAGCGGCTGTCG
GTCTGCGTGACAAGCAAAAA

Product: Na(+)-translocating NADH-quinone reductase subunit B

Products: NA

Alternate protein names: Na(+)-NQR subunit B; Na(+)-translocating NQR subunit B; NQR complex subunit B; NQR-1 subunit B [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLRQDRERRVISMGLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAM
FWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFC
MVRKHEVNEGFFVTSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTA
ADGYSGATALSQWAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILL
STLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILF
ANLFAPLFDHVVVERNIKRRLARYGKQ

Sequences:

>Translated_427_residues
MLRQDRERRVISMGLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAM
FWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFC
MVRKHEVNEGFFVTSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTA
ADGYSGATALSQWAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILL
STLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILF
ANLFAPLFDHVVVERNIKRRLARYGKQ
>Mature_427_residues
MLRQDRERRVISMGLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAM
FWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFC
MVRKHEVNEGFFVTSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTA
ADGYSGATALSQWAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILL
STLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILF
ANLFAPLFDHVVVERNIKRRLARYGKQ

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. NqrA to nqrE are probably involved in the second step, the conversion of ubisemiqui

COG id: COG1805

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrB/rnfD family [H]

Homologues:

Organism=Escherichia coli, GI1787917, Length=302, Percent_Identity=27.1523178807947, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010966
- InterPro:   IPR004338 [H]

Pfam domain/function: PF03116 NQR2_RnfD_RnfE [H]

EC number: NA

Molecular weight: Translated: 46899; Mature: 46899

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQDRERRVISMGLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
DSVDLKRIMIMVWLAVFPAMFWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEML
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
GGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTA
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCEEEEEEECCCCCCCEEEEE
ADGYSGATALSQWAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIV
CCCCCCHHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YMGIASWRIIGGVMIGMILLSTLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEHHHHHHHHHHHHHHCCCCC
SASFTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVVVERNIKRR
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARYGKQ
HHHHCCC
>Mature Secondary Structure
MLRQDRERRVISMGLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
DSVDLKRIMIMVWLAVFPAMFWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEML
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
GGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTA
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCEEEEEEECCCCCCCEEEEE
ADGYSGATALSQWAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIV
CCCCCCHHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YMGIASWRIIGGVMIGMILLSTLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEHHHHHHHHHHHHHHCCCCC
SASFTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVVVERNIKRR
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARYGKQ
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10077658; 10952301 [H]