Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 227082398

GI number: 227082398

Start: 2365274

End: 2366464

Strand: Reverse

Name: proB [H]

Synonym: VCM66_2197

Alternate gene names: 227082398

Gene position: 2366464-2365274 (Counterclockwise)

Preceding gene: 227082399

Following gene: 227082397

Centisome position: 81.81

GC content: 51.55

Gene sequence:

>1191_bases
TTGCAGTGTGGATTGCTAAAACATCGTCTCTTAAAGTTTTTCTCAACAGAAGCAATCATGACCACAAATCAACGAAGCAT
CAAAGCTCCACAAACGGTTGTTGTGAAGCTGGGAACCAGTGTTCTGACCGGCGGTACTCTTGCGCTGGATCGTGCTCATA
TGGTTGAGCTCGCTCGCCAGTGTGCTGAACTCAAAAAGCAAGGTCACTCAGTAGTGATCGTGACCTCGGGCGCCATTGCG
GCTGGCCGTGAGCATTTAGGTTACCCTGCGCTGCCCAACGCGATTTCCAGCAAGCAACTGCTGGCAGCGGTTGGGCAAAG
TCGTTTGATTCAGGTCTGGGAATCGTTATTTGCGATTTATGGCATCAAAATTGGTCAGATGCTGCTCACCCGTGCTGATC
TTGATGACCGTGAGCGTTTCCTTAATGCGCGTGACACCATCAATGCGTTAGTTGAAAATGGCATTGTGCCTGTGGTCAAC
GAAAACGATGCGGTAGCAACCAATGAAATCAAAGTCGGTGACAACGATAACTTATCGGCGCTGGTCGGTATTTTGTGTGG
TGCGGATAAGCTGCTGCTGTTGACCGATCAAAAAGGCTTGTTCACCGCCGATCCGCGTAAAGATCCCAATGCCCAACTGA
TCAAAGAAGTGACCACCATTGATGACACACTGCGCAAAATTGCTGGTGGCAGTGGCACTACACTCGGTACGGGCGGCATG
GCCACCAAGCTGCAAGCGGCGGACATTGCTCGTCGTGCTGGTATTGAAGTCATCATTGCGGCAGGTCGTGCGCCCAATGT
GATTTTTGACTCCTTAAGTGATGAGCCACAAGGCACGCGCTTCTTAGCTTTGGAAGAAGCGTTAGAAAACCGCAAACGCT
GGATTTTGGCAGGCCCAGCGGCATCAGGCGATATTGTGATTGACGATGGCGCAGTGAAAGCGGTGCAAACCAAGGGCAGC
AGCTTGCTCGCCAAAGGTGTGGTGCGAGTGCAAGGTCAGTTCGCCCGTGGTGAAGTGGTGCGGGTACTGGATAAGCAAAG
TCATCTGATTGCGCGCGGCATCGCGGCCTACTCCAATGACGAGTTGGCGCAAATTGCTGGTAAGCACAGTAAAGAGATCA
TCGATATTTTAGGCTACGATCACGGCTCAGAAGTGATCCACCGCGACGACATGGTCGTGATTCAAGAGTAA

Upstream 100 bases:

>100_bases
TGTCACTGTCTCAACCGAGTCCGCGCCATTTGCCTAATTAACCCTGTTCAATCGATAACCCGAAGAAAAAGGTGCTTTTG
CACCTTTTTCTGCTTGTGCA

Downstream 100 bases:

>100_bases
AGGAGAGAATGATGGATTTAACCGTACTCGGTAAAGCGGCCAAAGCGGCCTCTTTCCAACTGGCTACCGCCAGTACCGCG
CAAAAAAATCAGGCTCTCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MQCGLLKHRLLKFFSTEAIMTTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVIVTSGAIA
AGREHLGYPALPNAISSKQLLAAVGQSRLIQVWESLFAIYGIKIGQMLLTRADLDDRERFLNARDTINALVENGIVPVVN
ENDAVATNEIKVGDNDNLSALVGILCGADKLLLLTDQKGLFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGM
ATKLQAADIARRAGIEVIIAAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPAASGDIVIDDGAVKAVQTKGS
SLLAKGVVRVQGQFARGEVVRVLDKQSHLIARGIAAYSNDELAQIAGKHSKEIIDILGYDHGSEVIHRDDMVVIQE

Sequences:

>Translated_396_residues
MQCGLLKHRLLKFFSTEAIMTTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVIVTSGAIA
AGREHLGYPALPNAISSKQLLAAVGQSRLIQVWESLFAIYGIKIGQMLLTRADLDDRERFLNARDTINALVENGIVPVVN
ENDAVATNEIKVGDNDNLSALVGILCGADKLLLLTDQKGLFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGM
ATKLQAADIARRAGIEVIIAAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPAASGDIVIDDGAVKAVQTKGS
SLLAKGVVRVQGQFARGEVVRVLDKQSHLIARGIAAYSNDELAQIAGKHSKEIIDILGYDHGSEVIHRDDMVVIQE
>Mature_396_residues
MQCGLLKHRLLKFFSTEAIMTTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVIVTSGAIA
AGREHLGYPALPNAISSKQLLAAVGQSRLIQVWESLFAIYGIKIGQMLLTRADLDDRERFLNARDTINALVENGIVPVVN
ENDAVATNEIKVGDNDNLSALVGILCGADKLLLLTDQKGLFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGM
ATKLQAADIARRAGIEVIIAAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPAASGDIVIDDGAVKAVQTKGS
SLLAKGVVRVQGQFARGEVVRVLDKQSHLIARGIAAYSNDELAQIAGKHSKEIIDILGYDHGSEVIHRDDMVVIQE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=302, Percent_Identity=32.7814569536424, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI62912457, Length=300, Percent_Identity=32.6666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786437, Length=362, Percent_Identity=66.5745856353591, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17569967, Length=280, Percent_Identity=32.5, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=280, Percent_Identity=32.5, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=412, Percent_Identity=36.6504854368932, Blast_Score=229, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=36.5617433414044, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI21357643, Length=300, Percent_Identity=33, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 42369; Mature: 42369

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCGLLKHRLLKFFSTEAIMTTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQ
CCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHH
CAELKKQGHSVVIVTSGAIAAGREHLGYPALPNAISSKQLLAAVGQSRLIQVWESLFAIY
HHHHHHCCCEEEEEECCCHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIKIGQMLLTRADLDDRERFLNARDTINALVENGIVPVVNENDAVATNEIKVGDNDNLSA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHH
LVGILCGADKLLLLTDQKGLFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGM
HHHHHHCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
ATKLQAADIARRAGIEVIIAAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPA
HHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCCCEEEHHHHHHHCCCEEEEECCC
ASGDIVIDDGAVKAVQTKGSSLLAKGVVRVQGQFARGEVVRVLDKQSHLIARGIAAYSND
CCCCEEEECCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
ELAQIAGKHSKEIIDILGYDHGSEVIHRDDMVVIQE
HHHHHHCCHHHHHHHHHCCCCCCCEEECCCEEEEEC
>Mature Secondary Structure
MQCGLLKHRLLKFFSTEAIMTTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQ
CCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHH
CAELKKQGHSVVIVTSGAIAAGREHLGYPALPNAISSKQLLAAVGQSRLIQVWESLFAIY
HHHHHHCCCEEEEEECCCHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIKIGQMLLTRADLDDRERFLNARDTINALVENGIVPVVNENDAVATNEIKVGDNDNLSA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHH
LVGILCGADKLLLLTDQKGLFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGM
HHHHHHCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
ATKLQAADIARRAGIEVIIAAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPA
HHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCCCEEEHHHHHHHCCCEEEEECCC
ASGDIVIDDGAVKAVQTKGSSLLAKGVVRVQGQFARGEVVRVLDKQSHLIARGIAAYSND
CCCCEEEECCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
ELAQIAGKHSKEIIDILGYDHGSEVIHRDDMVVIQE
HHHHHHCCHHHHHHHHHCCCCCCCEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]