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The map label for this gene is cheR-2

Identifier: 227082325

GI number: 227082325

Start: 2284233

End: 2285060

Strand: Reverse

Name: cheR-2

Synonym: VCM66_2124

Alternate gene names: 227082325

Gene position: 2285060-2284233 (Counterclockwise)

Preceding gene: 227082326

Following gene: 227082324

Centisome position: 79.0

GC content: 46.38

Gene sequence:

>828_bases
ATGACTGCTATAACGATTAGCGACCAAGAGTATCGCGACTTTTGCCGATTTCTGGAATCTCAGTGCGGAATTGTACTGGG
TGACAGCAAGCAGTATTTGGTGCGAAGTCGTCTAAGTCCACTCGTCACCAAATTCAAGTTAAGTTCGTTGTCTGACTTGT
TGCGGGATGTCGTGACAGGACGAAATCGCGATCTGCGCGTAGCAGCGGTTGACGCCATGACAACCAACGAAACCTTATGG
TTTCGAGATTCCTACCCATTTACGGTTTTAGCTGACAAATTATTGCCAGAAATGGCGGCAAATAAACGGCCGATAAAAAT
CTGGTCGGCGGCCAGTTCATCAGGCCAAGAGCCGTACTCCATGGCGATGACGATTTTAGAAGTGCAGCAAAAACGTCCGG
GATTGTTGCCAAGCGTGTCAATTACTGCCACGGATATTTCAGCCAGTATGTTAGATATGTGCCGAGCCGGGATTTATGAC
AACTTAGCTTTAGGACGTGGTTTATCTCCTGAGCGTCGTCGAGTGTTTTTCGAAGATGCTGGGGATGGCCGGATGAAGGT
GAAGGATAACGTAAAACGTTTAGTGAACTTCCGTCCGCAAAACTTAATGGAAAGTTACTCCTTGCTGGGTAAGTTTGACA
TCATTTTTTGCCGTAACGTGCTGATCTATTTCTCGCCAGATATGAAATCTAAAGTGCTTAATCAGATGGCGGCAAGTTTG
AACCCCGGCGGCTATTTACTGTTAGGGGCATCCGAATCCCTGACTGGGCTGACGGATAAGTTTGAAATGGTGCGTTGTAA
CCCTGGTATCATCTATAAACTCAAATAA

Upstream 100 bases:

>100_bases
ATGGTTGAGAGAGTTGGGGCAAATGCGTTTATTGCGAAATTTAACCCTGATGAGCTTGGTAATGCGGTGAAAGCTGCGCT
AGTTAAATAAAGAGAATTGA

Downstream 100 bases:

>100_bases
AGAAATTAATCCATCTCATTTTGCCCGGTTTCTACCGGGCTTTTTCTTTTCTTTCAGACTCTAATGAACAGGAACGCCTT
TTTCTTGCAAGGAGTCGGTT

Product: chemotaxis protein methyltransferase CheR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTAITISDQEYRDFCRFLESQCGIVLGDSKQYLVRSRLSPLVTKFKLSSLSDLLRDVVTGRNRDLRVAAVDAMTTNETLW
FRDSYPFTVLADKLLPEMAANKRPIKIWSAASSSGQEPYSMAMTILEVQQKRPGLLPSVSITATDISASMLDMCRAGIYD
NLALGRGLSPERRRVFFEDAGDGRMKVKDNVKRLVNFRPQNLMESYSLLGKFDIIFCRNVLIYFSPDMKSKVLNQMAASL
NPGGYLLLGASESLTGLTDKFEMVRCNPGIIYKLK

Sequences:

>Translated_275_residues
MTAITISDQEYRDFCRFLESQCGIVLGDSKQYLVRSRLSPLVTKFKLSSLSDLLRDVVTGRNRDLRVAAVDAMTTNETLW
FRDSYPFTVLADKLLPEMAANKRPIKIWSAASSSGQEPYSMAMTILEVQQKRPGLLPSVSITATDISASMLDMCRAGIYD
NLALGRGLSPERRRVFFEDAGDGRMKVKDNVKRLVNFRPQNLMESYSLLGKFDIIFCRNVLIYFSPDMKSKVLNQMAASL
NPGGYLLLGASESLTGLTDKFEMVRCNPGIIYKLK
>Mature_274_residues
TAITISDQEYRDFCRFLESQCGIVLGDSKQYLVRSRLSPLVTKFKLSSLSDLLRDVVTGRNRDLRVAAVDAMTTNETLWF
RDSYPFTVLADKLLPEMAANKRPIKIWSAASSSGQEPYSMAMTILEVQQKRPGLLPSVSITATDISASMLDMCRAGIYDN
LALGRGLSPERRRVFFEDAGDGRMKVKDNVKRLVNFRPQNLMESYSLLGKFDIIFCRNVLIYFSPDMKSKVLNQMAASLN
PGGYLLLGASESLTGLTDKFEMVRCNPGIIYKLK

Specific function: Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain

Homologues:

Organism=Escherichia coli, GI1788193, Length=266, Percent_Identity=32.3308270676692, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHER1_VIBCH (Q9KQ06)

Other databases:

- EMBL:   AE003852
- EMBL:   AF139167
- PIR:   H82106
- RefSeq:   NP_231832.1
- ProteinModelPortal:   Q9KQ06
- SMR:   Q9KQ06
- GeneID:   2613241
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2201
- TIGR:   VC_2201
- HOGENOM:   HBG661948
- OMA:   DIVYCRN
- ProtClustDB:   CLSK874707
- BRENDA:   2.1.1.80
- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- Gene3D:   G3DSA:1.10.155.10
- PRINTS:   PR00996
- SMART:   SM00138

Pfam domain/function: PF01739 CheR; PF03705 CheR_N; SSF47757 CheR_mtfrase

EC number: =2.1.1.80

Molecular weight: Translated: 30871; Mature: 30740

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50123 CHER

Important sites: BINDING 76-76 BINDING 78-78 BINDING 82-82 BINDING 117-117 BINDING 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITISDQEYRDFCRFLESQCGIVLGDSKQYLVRSRLSPLVTKFKLSSLSDLLRDVVTG
CEEEEECCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RNRDLRVAAVDAMTTNETLWFRDSYPFTVLADKLLPEMAANKRPIKIWSAASSSGQEPYS
CCCCEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
MAMTILEVQQKRPGLLPSVSITATDISASMLDMCRAGIYDNLALGRGLSPERRRVFFEDA
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHEEEEECC
GDGRMKVKDNVKRLVNFRPQNLMESYSLLGKFDIIFCRNVLIYFSPDMKSKVLNQMAASL
CCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCEEEEECCCHHHHHHHHHHHHC
NPGGYLLLGASESLTGLTDKFEMVRCNPGIIYKLK
CCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEC
>Mature Secondary Structure 
TAITISDQEYRDFCRFLESQCGIVLGDSKQYLVRSRLSPLVTKFKLSSLSDLLRDVVTG
EEEEECCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RNRDLRVAAVDAMTTNETLWFRDSYPFTVLADKLLPEMAANKRPIKIWSAASSSGQEPYS
CCCCEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
MAMTILEVQQKRPGLLPSVSITATDISASMLDMCRAGIYDNLALGRGLSPERRRVFFEDA
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHEEEEECC
GDGRMKVKDNVKRLVNFRPQNLMESYSLLGKFDIIFCRNVLIYFSPDMKSKVLNQMAASL
CCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCEEEEECCCHHHHHHHHHHHHC
NPGGYLLLGASESLTGLTDKFEMVRCNPGIIYKLK
CCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301; 10400589