Click here to switch to the map view.

The map label for this gene is flrA [H]

Identifier: 227082262

GI number: 227082262

Start: 2228333

End: 2229790

Strand: Reverse

Name: flrA [H]

Synonym: VCM66_2060

Alternate gene names: 227082262

Gene position: 2229790-2228333 (Counterclockwise)

Preceding gene: 227082263

Following gene: 227082261

Centisome position: 77.09

GC content: 49.86

Gene sequence:

>1458_bases
ATGCAGAGTTTAGCGAAACTACTTGTGATTGAGGACGATGCCGCGATCCGGCTCAATCTCAGTGTTATTTTAGAATTTGT
AGGAGAGCAGTGTGAGGTCATTGAGTCTACTCAGATTGACCAAATCAATTGGTCGGCAGTGTGGGGTGGGTGCATTTTAG
GCTCGCTACGTGGTCAAGCGCTTTCTGAACAATTAATCCAATCTTTAAACCATATCCCGTTGCTGGTTGCCAATAAGCAG
CCTTATTCCTTAGAAGAATTCCCCAACTATGTGGGTGAGTTAGATTTTCCGCTCAACTATCCGCAGCTGAGCGATGCTTT
GCGTCACTGTAAAGAATTTCTTGGGCGCAAAGGTTTTCAAGTCCTTGCGACCGCACGCAAAAACACCCTGTTTCGTAGCC
TTGTTGGGCAAAGCATGGGGATTCAAGAAGTTCGCCACTTGATTGAGCAAGTCTCGACCACGGAAGCCAACGTGCTGATC
CTCGGTGAGTCGGGCACGGGTAAAGAAGTGGTTGCGCGTAACATTCACTACCATTCAGGACGCCGTAATGGCCCTTTTGT
CCCCATCAACTGTGGTGCAATCCCGGCTGAATTGCTGGAAAGTGAACTGTTTGGTCATGAAAAAGGGGCGTTTACCGGAG
CGATTACCGCACGTAAAGGCCGTTTTGAACTGGCTGAAGGCGGCACACTCTTTTTAGATGAAATTGGCGATATGCCGATG
AGCATGCAGGTTAAACTGCTGCGCGTGCTGCAAGAGCGCTGTTTTGAACGGGTGGGAGGCAACAGCACCATCAAAGCGAA
TGTGCGCGTGATTGCCGCAACGCACCGTAATTTGGAAGAGATGATTGACGGACAGAAATTTCGTGAAGATCTCTACTACC
GCTTAAACGTGTTCCCGATTGAAATGCCCGCTCTGCGCGATCGGATTGATGACATTCCTTTGCTGTTACAAGAGCTGATG
ACTCGTATGGAAGCGGAAGGGGCGCAGCCCATCTGTTTCACGCCGCGTGCGATCAATTCGATGATGGAGCATGACTGGCC
GGGTAATGTGCGTGAACTTGCCAACTTGGTTGAGCGTATGGTCATCCTGTATCCAAACAGTTTGGTCGATGTGAATCACC
TGCCGACCAAATACCGTTACAGTGATATTCCGGAATTCCAGCCAGAGCCTAGCCGTTTTAGCTCGGTTGAAGAGCAAGAG
CGTGATGTGTTGGAAGGCATTTTTGCAGAAGACTTTAATTTTGAAGAGCCGCAGGAGTTTGTCCCTGATATAGATGCGCC
GCAAGCGCTGCCACCTGAAGGGGTGAATCTCAAAGAGTTGCTCGCTGATTTAGAAGTGAACCTCATCAACCAAGCGTTAG
AAGCGCAAGGGGGGGTAGTGGCGAGAGCGGCAGACATGCTCGGTATGCGTCGCACCACCTTGGTTGAGAAGATGCGCAAA
TACAACATGCAACGCTAG

Upstream 100 bases:

>100_bases
CTATCGTTTATTCAGGATCCAGAATCAAAATTAACACCTGAAAATGATGTTCAAACGGTGCAACCACAACTTCTTATAAT
AAAGGCAAATAATCTCACCT

Downstream 100 bases:

>100_bases
GGAAACCATAGTCAATATATTGGCATTAATTAACGTGTTGATTTTAAACATGAATGGCTTGGCATGACTCTTGCGTGCCA
AGCCATTGTTGCATTTAAAA

Product: flagellar regulatory protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQALSEQLIQSLNHIPLLVANKQ
PYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRKGFQVLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEANVLI
LGESGTGKEVVARNIHYHSGRRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGDMPM
SMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNVFPIEMPALRDRIDDIPLLLQELM
TRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLVERMVILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVEEQE
RDVLEGIFAEDFNFEEPQEFVPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEKMRK
YNMQR

Sequences:

>Translated_485_residues
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQALSEQLIQSLNHIPLLVANKQ
PYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRKGFQVLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEANVLI
LGESGTGKEVVARNIHYHSGRRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGDMPM
SMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNVFPIEMPALRDRIDDIPLLLQELM
TRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLVERMVILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVEEQE
RDVLEGIFAEDFNFEEPQEFVPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEKMRK
YNMQR
>Mature_485_residues
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQALSEQLIQSLNHIPLLVANKQ
PYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRKGFQVLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEANVLI
LGESGTGKEVVARNIHYHSGRRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGDMPM
SMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNVFPIEMPALRDRIDDIPLLLQELM
TRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLVERMVILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVEEQE
RDVLEGIFAEDFNFEEPQEFVPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEKMRK
YNMQR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=377, Percent_Identity=42.9708222811671, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788550, Length=335, Percent_Identity=42.9850746268657, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1789087, Length=359, Percent_Identity=40.6685236768802, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790437, Length=423, Percent_Identity=39.4799054373522, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1790299, Length=495, Percent_Identity=34.5454545454545, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788905, Length=228, Percent_Identity=53.5087719298246, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1789233, Length=355, Percent_Identity=40, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=46.7532467532467, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1786524, Length=252, Percent_Identity=43.6507936507937, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87081872, Length=355, Percent_Identity=35.7746478873239, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=246, Percent_Identity=39.4308943089431, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789828, Length=235, Percent_Identity=34.468085106383, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI87081858, Length=338, Percent_Identity=27.5147928994083, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54692; Mature: 54692

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQA
CCCHHEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LSEQLIQSLNHIPLLVANKQPYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRKGFQ
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
VLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEANVLILGESGTGKEVVARNIHYHSG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCHHCCC
RRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGDMPM
CCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCEEEEHHHCCCCH
SMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNVFPI
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCHHHHHCHHHHHHHHHEEEEEEEE
EMPALRDRIDDIPLLLQELMTRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLVERM
CCHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHH
VILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVEEQERDVLEGIFAEDFNFEEPQEF
HHHCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEKMRK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
YNMQR
HCCCC
>Mature Secondary Structure
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQA
CCCHHEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LSEQLIQSLNHIPLLVANKQPYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRKGFQ
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
VLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEANVLILGESGTGKEVVARNIHYHSG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCHHCCC
RRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGDMPM
CCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCEEEEHHHCCCCH
SMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNVFPI
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCHHHHHCHHHHHHHHHEEEEEEEE
EMPALRDRIDDIPLLLQELMTRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLVERM
CCHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHH
VILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVEEQERDVLEGIFAEDFNFEEPQEF
HHHCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEKMRK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
YNMQR
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]