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The map label for this gene is flhB [H]

Identifier: 227082245

GI number: 227082245

Start: 2212125

End: 2213255

Strand: Reverse

Name: flhB [H]

Synonym: VCM66_2043

Alternate gene names: 227082245

Gene position: 2213255-2212125 (Counterclockwise)

Preceding gene: 227082246

Following gene: 227082243

Centisome position: 76.52

GC content: 50.4

Gene sequence:

>1131_bases
TTGGCAGAATCCGACGGACAAGAAAGGACTGAAGAAGCCACGCCCCGAAGGTTGCAGCAGGCCAAGGAAAAAGGCCAAGT
TGCGCGTTCGAAGGAGTTGGCTTCTGTCTCGGTATTGGTAGTAGGTGCGGTTTCCTTGATGTGGTTTGGCGAGATCTTGG
CCAAAGGATTGATGGTGGCCATGCAGCGCCTTTTTGAGCTAAGCCGTGAAGAGATTTTCGATCTTGGTAAGCTGTTTGAC
ATCATTGGTGGCTCACTAGTCAACCTGCTGTTGCCCTTGCTCATGATCCTGAGCACCTTGTTTATCGCTGCCTTAATCGG
TGCGGCGGGGGTCGGTGGGATCAGTTTTTCCGCTGAAGCGGCGATGCCTAAGCTGTCGAAAATGAATCCGCTCAGTGGCT
TAAAGCGAATGTTTGGTATGCAAAGCTGGGTCGAGCTGCTCAAATCCATCTTGAAAGTGTTGCTGGTGTCTGGCGTCGCT
TTTTATCTGATTGAAGCTTCGCAAAAGGATCTGTTCCAGCTCAGTCTTGATGTGTATCCGCAAAACATCTTTCATGCGCT
GGATATTCTGCTCAACTTTGTGTTGCTGATCAGTTGTTCGTTACTGGTGGTGGTGGCGATTGATATTCCATTCCAGATTT
GGCAGCACGCTGAACAACTGAAAATGACCAAACAGGAAGTCAAAGACGAGTACAAAGACACCGAAGGTAAACCAGAGGTG
AAAGGGCGCATTCGTATGTTGCAACGTGAAGCGGCGCAGCGACGTATGATGGCTGCACTGCCACAGGCCGATGTCATCAT
CACTAACCCTGAGCACTTTTCGGTAGCACTGCGTTATAAGCAAAACACCGATAAAGCGCCGGTGGTGATAGCTAAAGGTG
TCGATCATATGGCACTCAAGATCCGTGAAATCGCGCGGGAATATGACATTGCGATTGTTCCTGCACCGCCCTTAGCACGC
GCCTTGTACCATACGACCGAGCTGGAGCAGCAAATTCCGGATGGCCTATTCGTGGCCGTCGCTCAAGTGTTGGCATTTGT
GTTCCAGCTTAAACAGTATCGCCGCAAAGGTGGTCAGCGACCGAAACTCAATGAGGAGAATATGCCGATCCCACCGGATA
TGCGTTACTAA

Upstream 100 bases:

>100_bases
GTACATCGTCCACGGTTTGTACACCCATTACGATATTTATTGGCAAGAGGCGGAAGAGCAAGTTTGCCGTCTCATTCGCT
TAAACTGTTAGGAGGCTGAT

Downstream 100 bases:

>100_bases
AGATGCGCTATTTTTCAGGAATCGACAGCAGTACCAGTAGCGCGCCATCACCGCCAAACTCAAGTGGGGCTTGGTGGAAC
GCCAGCACATCGGGGTGCTG

Product: flagellar biosynthesis protein FlhB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAESDGQERTEEATPRRLQQAKEKGQVARSKELASVSVLVVGAVSLMWFGEILAKGLMVAMQRLFELSREEIFDLGKLFD
IIGGSLVNLLLPLLMILSTLFIAALIGAAGVGGISFSAEAAMPKLSKMNPLSGLKRMFGMQSWVELLKSILKVLLVSGVA
FYLIEASQKDLFQLSLDVYPQNIFHALDILLNFVLLISCSLLVVVAIDIPFQIWQHAEQLKMTKQEVKDEYKDTEGKPEV
KGRIRMLQREAAQRRMMAALPQADVIITNPEHFSVALRYKQNTDKAPVVIAKGVDHMALKIREIAREYDIAIVPAPPLAR
ALYHTTELEQQIPDGLFVAVAQVLAFVFQLKQYRRKGGQRPKLNEENMPIPPDMRY

Sequences:

>Translated_376_residues
MAESDGQERTEEATPRRLQQAKEKGQVARSKELASVSVLVVGAVSLMWFGEILAKGLMVAMQRLFELSREEIFDLGKLFD
IIGGSLVNLLLPLLMILSTLFIAALIGAAGVGGISFSAEAAMPKLSKMNPLSGLKRMFGMQSWVELLKSILKVLLVSGVA
FYLIEASQKDLFQLSLDVYPQNIFHALDILLNFVLLISCSLLVVVAIDIPFQIWQHAEQLKMTKQEVKDEYKDTEGKPEV
KGRIRMLQREAAQRRMMAALPQADVIITNPEHFSVALRYKQNTDKAPVVIAKGVDHMALKIREIAREYDIAIVPAPPLAR
ALYHTTELEQQIPDGLFVAVAQVLAFVFQLKQYRRKGGQRPKLNEENMPIPPDMRY
>Mature_375_residues
AESDGQERTEEATPRRLQQAKEKGQVARSKELASVSVLVVGAVSLMWFGEILAKGLMVAMQRLFELSREEIFDLGKLFDI
IGGSLVNLLLPLLMILSTLFIAALIGAAGVGGISFSAEAAMPKLSKMNPLSGLKRMFGMQSWVELLKSILKVLLVSGVAF
YLIEASQKDLFQLSLDVYPQNIFHALDILLNFVLLISCSLLVVVAIDIPFQIWQHAEQLKMTKQEVKDEYKDTEGKPEVK
GRIRMLQREAAQRRMMAALPQADVIITNPEHFSVALRYKQNTDKAPVVIAKGVDHMALKIREIAREYDIAIVPAPPLARA
LYHTTELEQQIPDGLFVAVAQVLAFVFQLKQYRRKGGQRPKLNEENMPIPPDMRY

Specific function: Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with fliI and fliH, may constitute the export apparatus of flagellin [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=370, Percent_Identity=43.5135135135135, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006136
- InterPro:   IPR006135 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 42070; Mature: 41939

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AESDGQERTEEATPRRLQQAKEKGQVARSKELASVSVLVVGAVSLMWFGEILAKGLMVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQRLFELSREEIFDLGKLFDIIGGSLVNLLLPLLMILSTLFIAALIGAAGVGGISFSAEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AMPKLSKMNPLSGLKRMFGMQSWVELLKSILKVLLVSGVAFYLIEASQKDLFQLSLDVYP
HCCHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QNIFHALDILLNFVLLISCSLLVVVAIDIPFQIWQHAEQLKMTKQEVKDEYKDTEGKPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
KGRIRMLQREAAQRRMMAALPQADVIITNPEHFSVALRYKQNTDKAPVVIAKGVDHMALK
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCEEEECCCHHHHHH
IREIAREYDIAIVPAPPLARALYHTTELEQQIPDGLFVAVAQVLAFVFQLKQYRRKGGQR
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PKLNEENMPIPPDMRY
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002587; 11677609; 3298217 [H]