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The map label for this gene is flhF [H]

Identifier: 227082195

GI number: 227082195

Start: 2159829

End: 2161316

Strand: Reverse

Name: flhF [H]

Synonym: VCM66_1992

Alternate gene names: 227082195

Gene position: 2161316-2159829 (Counterclockwise)

Preceding gene: 227082196

Following gene: 227082194

Centisome position: 74.72

GC content: 50.27

Gene sequence:

>1488_bases
TTGAAAATAAAACGATTTTTTGCCAAGGATATGAAAACTGCGCTGCTTCAGGTCAAAGAAGAGCTGGGCGTGGATGCTGT
GATCATGTCGAATAAAAAAGTCGCGGGTGGGGTCGAAATTGTGGCAGCGGTTGATGGCGATACTGCGCCCGCACCAGCCA
AGCGTTACAACTCGCAGCCTAGACATGGTTATAACCAAGTCTCACCCTCGGTCGCTCCGACCAAGAGCAGAGAGCTGGCG
GATGACCGTGTCAGTTTGCAATCGAGCGCCGACTCTGGCCGTTCTATGACTCAACGCTTTGCTAATATGCTCAAGCAATA
CAGCTCGGCAGATGAGCAAGAGCATCGTGCGGAAAACGAAGATTCGCTCTCTGCGCTGCTCAAGCGCCAGTCAGAAAATC
GTGCGCCACAACGTAATCAGACACAACGTAGTCAGAGTAGTTCAGAGCGTCAACGTCCTGACTCTCCGCTCGGTAAGTTG
CTGCAAGAAGATGCCGATGCACGCCGTAAGCCTAGACTCGATCCGACTCGTTATGATCGAAAAGTGCCTGAAGAGCCGAT
GGTCGCCGTCAGTGAATTAGAATCGATGCGTGAAGAGATGACCTCGATTCGCCGTTTGCTTGAGCATCAAGTTTCCGGAT
TAATGTGGCAAGAAGTCGAGCGTCGTGAGCCTCTGCGTGCCATGTTGATCAAACGCTTAGAGCGCATGGGTGTCTCTTTG
GAAATGGCGGACCAATTGGCTTGCTATATCCCAGAGGAAACGCCTCCTCCCAAAGCATGGAAAGCATTGTTAGGTTTAGT
CTCCGACCAAATTCCAGTCGTCAAACACGACATCCTAAAGCGTGGTGGCGTGGTTGCCTTACTTGGGCCAACGGGGGTAG
GGAAAACCACAACCGTGGCTAAATTGGCGGCTCGTGCAGCGATGGAATACGGCTCTGACAACGTCGCATTGGTGACCACA
GACACTTACCGTATTGGCGCCCATGAGCAATTGTCGATTTATGGCAGAATTATGGGATGTCCTGTAAGAGTTGCTAAAGA
TTCCAACGAGTTAGCCGATGTAATCTATCAGCTGCGTAATCGTCGCCTGATTTTAGTCGATACCGCAGGGATGGGACAAC
GCGATGTTCGGTTGTCAGAACAGCTGGATACCTTGATGCAAGAGAGCGGAGAGACTATCCATAGTTACCTTGTTTTGCCT
GCGACCGCGCAGCGCAAAGTATTACAAGAAACCATTGAGCATTTCAGAAGGATACCGCTTTCTGGATGCATTATGACCAA
ACTCGACGAATGTTTGAGCCTAGGCGAATTCGTCAGTGTGGTTGTACAAAATGCGTTGCCCGTCGCTTACATCGCGAATG
GACAACGAGTGCCCGAAGATATCGTAATCGCGCAACCCAAATACATGGTCGCAAAGGCAAACGAATTGCTAGAGAAGTCG
ACTGAAGATGAACCTCATTACTGGACCAGTGATTCAGAGAGATTCTAG

Upstream 100 bases:

>100_bases
GTGCTCTCCTATCAGGAGATCCCCGATGAGAAACAAATTCGAATTGTGCAGGCCGTGGGTAATTAATCGTCCGCACGTTT
TTTAAAGACGGAAATCTGCT

Downstream 100 bases:

>100_bases
GCGGCGGACAGGTATGACAAACAAAATGATATATGACCAAGCAAGCGGCCTACGTCGCTTAACCCAACCGTCAGTGACCA
AAGTAATTTCGGTCACTGGG

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKIKRFFAKDMKTALLQVKEELGVDAVIMSNKKVAGGVEIVAAVDGDTAPAPAKRYNSQPRHGYNQVSPSVAPTKSRELA
DDRVSLQSSADSGRSMTQRFANMLKQYSSADEQEHRAENEDSLSALLKRQSENRAPQRNQTQRSQSSSERQRPDSPLGKL
LQEDADARRKPRLDPTRYDRKVPEEPMVAVSELESMREEMTSIRRLLEHQVSGLMWQEVERREPLRAMLIKRLERMGVSL
EMADQLACYIPEETPPPKAWKALLGLVSDQIPVVKHDILKRGGVVALLGPTGVGKTTTVAKLAARAAMEYGSDNVALVTT
DTYRIGAHEQLSIYGRIMGCPVRVAKDSNELADVIYQLRNRRLILVDTAGMGQRDVRLSEQLDTLMQESGETIHSYLVLP
ATAQRKVLQETIEHFRRIPLSGCIMTKLDECLSLGEFVSVVVQNALPVAYIANGQRVPEDIVIAQPKYMVAKANELLEKS
TEDEPHYWTSDSERF

Sequences:

>Translated_495_residues
MKIKRFFAKDMKTALLQVKEELGVDAVIMSNKKVAGGVEIVAAVDGDTAPAPAKRYNSQPRHGYNQVSPSVAPTKSRELA
DDRVSLQSSADSGRSMTQRFANMLKQYSSADEQEHRAENEDSLSALLKRQSENRAPQRNQTQRSQSSSERQRPDSPLGKL
LQEDADARRKPRLDPTRYDRKVPEEPMVAVSELESMREEMTSIRRLLEHQVSGLMWQEVERREPLRAMLIKRLERMGVSL
EMADQLACYIPEETPPPKAWKALLGLVSDQIPVVKHDILKRGGVVALLGPTGVGKTTTVAKLAARAAMEYGSDNVALVTT
DTYRIGAHEQLSIYGRIMGCPVRVAKDSNELADVIYQLRNRRLILVDTAGMGQRDVRLSEQLDTLMQESGETIHSYLVLP
ATAQRKVLQETIEHFRRIPLSGCIMTKLDECLSLGEFVSVVVQNALPVAYIANGQRVPEDIVIAQPKYMVAKANELLEKS
TEDEPHYWTSDSERF
>Mature_495_residues
MKIKRFFAKDMKTALLQVKEELGVDAVIMSNKKVAGGVEIVAAVDGDTAPAPAKRYNSQPRHGYNQVSPSVAPTKSRELA
DDRVSLQSSADSGRSMTQRFANMLKQYSSADEQEHRAENEDSLSALLKRQSENRAPQRNQTQRSQSSSERQRPDSPLGKL
LQEDADARRKPRLDPTRYDRKVPEEPMVAVSELESMREEMTSIRRLLEHQVSGLMWQEVERREPLRAMLIKRLERMGVSL
EMADQLACYIPEETPPPKAWKALLGLVSDQIPVVKHDILKRGGVVALLGPTGVGKTTTVAKLAARAAMEYGSDNVALVTT
DTYRIGAHEQLSIYGRIMGCPVRVAKDSNELADVIYQLRNRRLILVDTAGMGQRDVRLSEQLDTLMQESGETIHSYLVLP
ATAQRKVLQETIEHFRRIPLSGCIMTKLDECLSLGEFVSVVVQNALPVAYIANGQRVPEDIVIAQPKYMVAKANELLEKS
TEDEPHYWTSDSERF

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1789874, Length=190, Percent_Identity=32.6315789473684, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 55434; Mature: 55434

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRFFAKDMKTALLQVKEELGVDAVIMSNKKVAGGVEIVAAVDGDTAPAPAKRYNSQP
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCHHHCCCCC
RHGYNQVSPSVAPTKSRELADDRVSLQSSADSGRSMTQRFANMLKQYSSADEQEHRAENE
CCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCH
DSLSALLKRQSENRAPQRNQTQRSQSSSERQRPDSPLGKLLQEDADARRKPRLDPTRYDR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHC
KVPEEPMVAVSELESMREEMTSIRRLLEHQVSGLMWQEVERREPLRAMLIKRLERMGVSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCH
EMADQLACYIPEETPPPKAWKALLGLVSDQIPVVKHDILKRGGVVALLGPTGVGKTTTVA
HHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHH
KLAARAAMEYGSDNVALVTTDTYRIGAHEQLSIYGRIMGCPVRVAKDSNELADVIYQLRN
HHHHHHHHHCCCCCEEEEEECCEECCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHC
RRLILVDTAGMGQRDVRLSEQLDTLMQESGETIHSYLVLPATAQRKVLQETIEHFRRIPL
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHHHHCCCC
SGCIMTKLDECLSLGEFVSVVVQNALPVAYIANGQRVPEDIVIAQPKYMVAKANELLEKS
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHEEECCCHHHHHHHHHHHHC
TEDEPHYWTSDSERF
CCCCCCEECCCCCCC
>Mature Secondary Structure
MKIKRFFAKDMKTALLQVKEELGVDAVIMSNKKVAGGVEIVAAVDGDTAPAPAKRYNSQP
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCHHHCCCCC
RHGYNQVSPSVAPTKSRELADDRVSLQSSADSGRSMTQRFANMLKQYSSADEQEHRAENE
CCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCH
DSLSALLKRQSENRAPQRNQTQRSQSSSERQRPDSPLGKLLQEDADARRKPRLDPTRYDR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHC
KVPEEPMVAVSELESMREEMTSIRRLLEHQVSGLMWQEVERREPLRAMLIKRLERMGVSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCH
EMADQLACYIPEETPPPKAWKALLGLVSDQIPVVKHDILKRGGVVALLGPTGVGKTTTVA
HHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHH
KLAARAAMEYGSDNVALVTTDTYRIGAHEQLSIYGRIMGCPVRVAKDSNELADVIYQLRN
HHHHHHHHHCCCCCEEEEEECCEECCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHC
RRLILVDTAGMGQRDVRLSEQLDTLMQESGETIHSYLVLPATAQRKVLQETIEHFRRIPL
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHHHHCCCC
SGCIMTKLDECLSLGEFVSVVVQNALPVAYIANGQRVPEDIVIAQPKYMVAKANELLEKS
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHEEECCCHHHHHHHHHHHHC
TEDEPHYWTSDSERF
CCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9503621 [H]