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The map label for this gene is fliA [H]

Identifier: 227082193

GI number: 227082193

Start: 2158201

End: 2158935

Strand: Reverse

Name: fliA [H]

Synonym: VCM66_1990

Alternate gene names: 227082193

Gene position: 2158935-2158201 (Counterclockwise)

Preceding gene: 227082194

Following gene: 227082192

Centisome position: 74.64

GC content: 45.44

Gene sequence:

>735_bases
GTGAATAAAGCGCTTACATACGATCAATACGGAAATCTTAATAGCCAGCAGCTATTTCTTGAGAAGTACTCTGTATTGGT
TAAGCGTATCGCTCATCATTTGATTGGTCGTCTTCCGCCCAGTGTTCTGATCGAAGACCTCATTCAAGCTGGCATGGTTG
GTTTGCTTGAGGCACAAAAAAATTATGATGGAAGTAAAGGCGCGAGCTTTGAAACCTATGCCGGAATTCGGATTCGTGGG
GCTATGCTCGACGATATCCGACGTGGTGACTGGGTTCCGCGATCTGTGCACAAACATAATCGGGAAATCAGTCAAGCGAT
TGCAGTGCTCGAGGGAGAACTCAATCGCGATCCAACGGATGCTGAAGTGGCGAAGTTTTTAAACATGTCACTAGAGCAGT
ATCACAGCGTGCTGATGGACATTAACTGTTCACGTATTGTTGGGATAGAGGATCTCGGTGTTTCTGACGACGCGATTTCA
CCGTTTGAAGAGGGGGAAGATAACTCGCCTTTTCACGGTGTCGCTGAAGAATCTTTCCGTAAAGCACTCGTTGAATCAAT
AAAATCGCTCCCGGAGCGTGAAGCTTTGGTACTTTCGCTCTATTATGATGAAGAGCTAAACTTAAAAGAAATTGGTGAGG
TACTTGGAGTTAGTGAATCTCGCGTCAGCCAGATACTCAGCCAATCTATGCAGCGGCTAAGAACAAAGTTAAGTTCTTGG
ACTCGGAATGACTAA

Upstream 100 bases:

>100_bases
AAAGCGTTGACTTGGCCGATACCGAGAACACCAAGTGGACATTTGGAGTTTTTCGTTGAACGCTTACTTAATCGGTCAGA
AACAGTAGGGGAACCTTTCG

Downstream 100 bases:

>100_bases
ATATCACTGATCTCAAACTCAGTGGAGGCAATTTTGAATAAAAACATGAAGATCCTTATTGTTGATGACTTTTCAACAAT
GCGCCGAATCGTTAAAAACC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNKALTYDQYGNLNSQQLFLEKYSVLVKRIAHHLIGRLPPSVLIEDLIQAGMVGLLEAQKNYDGSKGASFETYAGIRIRG
AMLDDIRRGDWVPRSVHKHNREISQAIAVLEGELNRDPTDAEVAKFLNMSLEQYHSVLMDINCSRIVGIEDLGVSDDAIS
PFEEGEDNSPFHGVAEESFRKALVESIKSLPEREALVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSSW
TRND

Sequences:

>Translated_244_residues
MNKALTYDQYGNLNSQQLFLEKYSVLVKRIAHHLIGRLPPSVLIEDLIQAGMVGLLEAQKNYDGSKGASFETYAGIRIRG
AMLDDIRRGDWVPRSVHKHNREISQAIAVLEGELNRDPTDAEVAKFLNMSLEQYHSVLMDINCSRIVGIEDLGVSDDAIS
PFEEGEDNSPFHGVAEESFRKALVESIKSLPEREALVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSSW
TRND
>Mature_244_residues
MNKALTYDQYGNLNSQQLFLEKYSVLVKRIAHHLIGRLPPSVLIEDLIQAGMVGLLEAQKNYDGSKGASFETYAGIRIRG
AMLDDIRRGDWVPRSVHKHNREISQAIAVLEGELNRDPTDAEVAKFLNMSLEQYHSVLMDINCSRIVGIEDLGVSDDAIS
PFEEGEDNSPFHGVAEESFRKALVESIKSLPEREALVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSSW
TRND

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=235, Percent_Identity=46.8085106382979, Blast_Score=196, Evalue=8e-52,
Organism=Escherichia coli, GI1789448, Length=202, Percent_Identity=29.2079207920792, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27465; Mature: 27465

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKALTYDQYGNLNSQQLFLEKYSVLVKRIAHHLIGRLPPSVLIEDLIQAGMVGLLEAQK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
NYDGSKGASFETYAGIRIRGAMLDDIRRGDWVPRSVHKHNREISQAIAVLEGELNRDPTD
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
AEVAKFLNMSLEQYHSVLMDINCSRIVGIEDLGVSDDAISPFEEGEDNSPFHGVAEESFR
HHHHHHHHHHHHHHHHHHHCCCCHHEECCHHCCCCCCCCCCHHCCCCCCCCCCHHHHHHH
KALVESIKSLPEREALVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSSW
HHHHHHHHHCCCHHHEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
TRND
CCCC
>Mature Secondary Structure
MNKALTYDQYGNLNSQQLFLEKYSVLVKRIAHHLIGRLPPSVLIEDLIQAGMVGLLEAQK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
NYDGSKGASFETYAGIRIRGAMLDDIRRGDWVPRSVHKHNREISQAIAVLEGELNRDPTD
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
AEVAKFLNMSLEQYHSVLMDINCSRIVGIEDLGVSDDAISPFEEGEDNSPFHGVAEESFR
HHHHHHHHHHHHHHHHHHHCCCCHHEECCHHCCCCCCCCCCHHCCCCCCCCCCHHHHHHH
KALVESIKSLPEREALVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSSW
HHHHHHHHHCCCHHHEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
TRND
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]