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The map label for this gene is purU [H]

Identifier: 227082119

GI number: 227082119

Start: 2080950

End: 2081783

Strand: Direct

Name: purU [H]

Synonym: VCM66_1916

Alternate gene names: 227082119

Gene position: 2080950-2081783 (Clockwise)

Preceding gene: 227082118

Following gene: 227082121

Centisome position: 71.94

GC content: 46.28

Gene sequence:

>834_bases
ATGGAAAAAAAAACCCTGCTGACGCATTGTCCTGATGCGCCGGGACTGATCTCCAAGATCACCAATATTTGTTACAAGCA
TCAACTCAACATCATCCACAATAATGAATTCGTGGATAATGCCAGCGGTCATTTTTTCATGCGTACCGAATTGGAAGGTT
ATTTCAATGACGCAACGCTGCTGGCCGACCTCGACCATGCTCTACCTCAAGGTACTAGGCGTAAACTGATCAGTTCGAGC
CGCAAACGCATCGTCATTCTGGTGACTAAAGAAGCGCACTGCTTAGGCGATATTTTGATGAAAAACTACGATGGTAGTCT
GGATGTGGATATTGCTGCGGTAGTCGGCAACTATGACACACTGCAACGTTTGACTGAGCGTTTTGATATTCCTTACCACT
GTGTGTCCCATGAAGGTTTGTCGCGTGAAGCTCACGAACAAGCACTGTTAGATGTGATTGACCAATATCAGCCAGATTAT
CTGGTTCTCGCCAAATATATGCGTGTGCTGACACCTGCGTTTGTGGAGCGTTTTCACCATAAAATCATCAATATCCACCA
CAGTTTCTTGCCCGCTTTTATCGGTGCGAAGCCGTATCAGCAAGCCTATGAGCGCGGTGTAAAAATCATTGGTGCAACAG
CGCATTTTGTGACGAACGATCTCGATGAAGGGCCGATCATCAAGCAAGACGTGATTCCGGTCGATCATACCTTTAGCGCG
CAAGATATGGCGCAAGCTGGGCGCGATGTAGAGAAGAATGTACTCAGTAAGGCTCTCAACAAAGTGCTGAATGATCATGT
GTTTGTCTACGGCAACAAGACCGTCATTTTGTAA

Upstream 100 bases:

>100_bases
GAAAAAATTCGCTAGCCCCAGACTGGCTGAAATGGCAAAAAATAGCCTTGTTATTTCAAAGAGAAAGGGGATTTTTATTT
TTGCATGGAAGTTGTTATCA

Downstream 100 bases:

>100_bases
CGAACCCTCAGCAGTAGACAATAACAAAGGGCACCGAAGTGCCCTTTGCCGTTCATGGTTTTCACTCAACAAGTTACAAT
CGAACTGCCAGCTCCACACC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MEKKTLLTHCPDAPGLISKITNICYKHQLNIIHNNEFVDNASGHFFMRTELEGYFNDATLLADLDHALPQGTRRKLISSS
RKRIVILVTKEAHCLGDILMKNYDGSLDVDIAAVVGNYDTLQRLTERFDIPYHCVSHEGLSREAHEQALLDVIDQYQPDY
LVLAKYMRVLTPAFVERFHHKIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHTFSA
QDMAQAGRDVEKNVLSKALNKVLNDHVFVYGNKTVIL

Sequences:

>Translated_277_residues
MEKKTLLTHCPDAPGLISKITNICYKHQLNIIHNNEFVDNASGHFFMRTELEGYFNDATLLADLDHALPQGTRRKLISSS
RKRIVILVTKEAHCLGDILMKNYDGSLDVDIAAVVGNYDTLQRLTERFDIPYHCVSHEGLSREAHEQALLDVIDQYQPDY
LVLAKYMRVLTPAFVERFHHKIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHTFSA
QDMAQAGRDVEKNVLSKALNKVLNDHVFVYGNKTVIL
>Mature_277_residues
MEKKTLLTHCPDAPGLISKITNICYKHQLNIIHNNEFVDNASGHFFMRTELEGYFNDATLLADLDHALPQGTRRKLISSS
RKRIVILVTKEAHCLGDILMKNYDGSLDVDIAAVVGNYDTLQRLTERFDIPYHCVSHEGLSREAHEQALLDVIDQYQPDY
LVLAKYMRVLTPAFVERFHHKIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHTFSA
QDMAQAGRDVEKNVLSKALNKVLNDHVFVYGNKTVIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=28.4210526315789, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=28.4210526315789, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=28.4210526315789, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=72.202166064982, Blast_Score=423, Evalue=1e-120,
Organism=Escherichia coli, GI1788846, Length=94, Percent_Identity=46.8085106382979, Blast_Score=94, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=176, Percent_Identity=33.5227272727273, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24582400, Length=202, Percent_Identity=29.7029702970297, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31373; Mature: 31373

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKTLLTHCPDAPGLISKITNICYKHQLNIIHNNEFVDNASGHFFMRTELEGYFNDATL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEEEEECCCCCCCHHH
LADLDHALPQGTRRKLISSSRKRIVILVTKEAHCLGDILMKNYDGSLDVDIAAVVGNYDT
HHHHHHHCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEHHEECCCHHH
LQRLTERFDIPYHCVSHEGLSREAHEQALLDVIDQYQPDYLVLAKYMRVLTPAFVERFHH
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHTFSA
HHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCHHCCCCCCCCCCH
QDMAQAGRDVEKNVLSKALNKVLNDHVFVYGNKTVIL
HHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEC
>Mature Secondary Structure
MEKKTLLTHCPDAPGLISKITNICYKHQLNIIHNNEFVDNASGHFFMRTELEGYFNDATL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEEEEECCCCCCCHHH
LADLDHALPQGTRRKLISSSRKRIVILVTKEAHCLGDILMKNYDGSLDVDIAAVVGNYDT
HHHHHHHCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEHHEECCCHHH
LQRLTERFDIPYHCVSHEGLSREAHEQALLDVIDQYQPDYLVLAKYMRVLTPAFVERFHH
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHTFSA
HHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCHHCCCCCCCCCCH
QDMAQAGRDVEKNVLSKALNKVLNDHVFVYGNKTVIL
HHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]