Click here to switch to the map view.

The map label for this gene is pyrF [H]

Identifier: 227082041

GI number: 227082041

Start: 1997896

End: 1998591

Strand: Reverse

Name: pyrF [H]

Synonym: VCM66_1835

Alternate gene names: 227082041

Gene position: 1998591-1997896 (Counterclockwise)

Preceding gene: 227082042

Following gene: 227082038

Centisome position: 69.1

GC content: 48.42

Gene sequence:

>696_bases
ATGAACGACCCAAAAGTGATTGTTGCACTGGACTATGACAATCTGGCGGATGCGCTCGCGTTTGTGGACAAAATTGACCC
AAGCACATGTCGACTCAAAGTGGGTAAAGAGATGTTTACTCTATTTGGTCCTGATTTTGTCCGTGAGCTGCATAAACGAG
GTTTTTCTGTGTTTCTGGATCTTAAATTTCACGATATTCCGAATACCTGTTCAAAAGCGGTGAAAGCTGCCGCTGAGCTT
GGGGTTTGGATGGTGAATGTGCACGCGAGTGGTGGCGAGCGAATGATGGCAGCATCGCGTGAGATTCTGGAGCCGTATGG
TAAAGAGCGCCCACTGCTGATTGGTGTCACTGTATTGACCAGTATGGAATCTGCCGATTTACAAGGTATTGGCATCTTGA
GTGCGCCACAAGATCATGTGCTGCGATTAGCAACGCTCACCAAAAATGCTGGTCTTGATGGTGTGGTATGCTCCGCACAA
GAGGCATCACTATTGAAGCAACATTTGGGGCGTGAATTCAAATTGGTGACGCCAGGAATTCGTCCAGCGGGTTCAGAGCA
AGGCGATCAACGCCGTATTATGACTCCAGCCCAAGCCATTGCCTCTGGTTCAGATTATCTCGTGATCGGTCGCCCTATCA
CTCAAGCCGCACATCCTGAAGTGGTTCTGGAAGAGATAAACAGCTCTTTAGTTTAG

Upstream 100 bases:

>100_bases
GCCAATCAGAGGCTTAGATGGTGAGTAATTAAAAATCAACAGTATGACGCAGCTATTTATGGCTGCTTTTTTTTAACTCG
ATATACAGATAGGAGAAACG

Downstream 100 bases:

>100_bases
CTTTACTAGATTGGATAAACTCAAGCCCAGTCACAGTACTGGGCTTTTTTAAAAAGGCTATTTAGGAATACCACTGTGAA
ACTTGAAATCGCTATCGGGA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVKAAAEL
GVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLTSMESADLQGIGILSAPQDHVLRLATLTKNAGLDGVVCSAQ
EASLLKQHLGREFKLVTPGIRPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV

Sequences:

>Translated_231_residues
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVKAAAEL
GVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLTSMESADLQGIGILSAPQDHVLRLATLTKNAGLDGVVCSAQ
EASLLKQHLGREFKLVTPGIRPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV
>Mature_231_residues
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVKAAAEL
GVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLTSMESADLQGIGILSAPQDHVLRLATLTKNAGLDGVVCSAQ
EASLLKQHLGREFKLVTPGIRPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=229, Percent_Identity=70.3056768558952, Blast_Score=325, Evalue=2e-90,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25003; Mature: 25003

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLD
CCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEECHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVKAAAELGVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHH
SMESADLQGIGILSAPQDHVLRLATLTKNAGLDGVVCSAQEASLLKQHLGREFKLVTPGI
CCCCCCCCCEEEECCCHHHHEEHHHHHCCCCCCCEEECHHHHHHHHHHCCCEEEEECCCC
RPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV
CCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLD
CCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEECHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVKAAAELGVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHH
SMESADLQGIGILSAPQDHVLRLATLTKNAGLDGVVCSAQEASLLKQHLGREFKLVTPGI
CCCCCCCCCEEEECCCHHHHEEHHHHHCCCCCCCEEECHHHHHHHHHHCCCEEEEECCCC
RPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV
CCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA