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The map label for this gene is hlyA [H]

Identifier: 227082018

GI number: 227082018

Start: 1972202

End: 1974277

Strand: Direct

Name: hlyA [H]

Synonym: VCM66_1812

Alternate gene names: 227082018

Gene position: 1972202-1974277 (Clockwise)

Preceding gene: 227082017

Following gene: 227082019

Centisome position: 68.18

GC content: 47.74

Gene sequence:

>2076_bases
ATGAAACAGACAAAAACGTTGACCGCGATAAGCGTTCTCGCGTTGAGCCATTTAATGACTCAAAGCACCGCATTCGCGTC
AAGCAGCAGTGATATACAGACCAAATTGAAATGGTCTTGGTCTACCAGCGTTTTTCATCCTGAGTCCAATCAGGTGATGG
CCGCGCCAATTGTCGTACAACTCAACGACGATAATGGCGATGGCAAAATTGATGAGAAAGATGTGGCTGACATTATTGTG
GTCACCTTTGAAGGTAATAAGTACGCGAATGGTGGTTATATCCGCGCCCTGAGCGGTGTGGATGGCTCTGAATTGTGGAG
TTATAGCAATGGAGGCGTGATTGCCGATGCTCGCTATGCACCAGCAGCCGCGGATCTCGATGGTGACGGACTGATTGAAA
TCGTCTCCACCAGCGCGTTGACACCTTATATCAATATTCTGGACCACCAAGGCAACATCAAAAAGCAACTGCTGAAATCT
GCTTCAGGCTGGCGTTCTGTAGGTGATATTGCACTGGCAGACATTAATGGCGATGGCAACATTGAAATTCTGGCTGCCGA
TGGTGTTTATAGTTACGAATCAGGCTTGCTCTTTAGTCATGATTGGGCACCAAGTTCGATTGCGTTTGATAGCAATGGCG
ATGGCCAACGTGAAGTCTTTGCCAACGGTACACTCTATCAAAACAACGGCGCTTACCTATGGCAATACCAAGCCAATGAC
ACCGTTTGGTTCTCTTCCGTAGCAAACCTTGATGGCGATGATAAGCCTGAACTGGTCGTGTCGGTTCCAGCGTCTTTGAG
CACACCCGAGAACAGTGAAATCGCTGTGCTTGAGCATGATGGCTCAGTGAAATGGCGAGTCAACAACCTATCTAACCCAG
GTGGTAGCGTACAAGCCGTCTCCAGTTTTCTGGGTAAACCAAGCTCCAGTGCAACCACTGTTGATGCTCAGTCTGCCGTT
TATGGCTACACCGACTGGGCACATCAACAACGTGTTCTCGCAGAAAATCATCAGTTAGCGATTCGTTCTGGTGCTGTAGT
CGATGCAATTGGTGCTAATAGCCAAAACATGATCGGCGGCAGCGGTGGCTCACTGAGCACAATTGATACCAGTAAAGTCC
GTGCCATTGATGTGACTTACGGTAAAAACAAATACACCTGGAAGTATGGTGTCCTCGAGATGAGTTTTACTCTCGATAAC
GGCGCAAAAGTCACTGTGGGGTCAAAAGATTCTGCGTTTACTTATCTAGGATTAGAGTGGAAAACTAAAACGGTTCCTTA
TCTAGGTGTTGAGTGGCGTACCAAAACCGTCTCTTACTGGTTCTTTGGCTGGCACACTAAACAAGTGGCTTATTTAGCGC
CAGTATGGAAAGAGAAAACCATCCCTTATGCCGTTCCTGTGACACTGTCGAAATCGACCACAGTACGCTATGACATTCCA
CAAGGCTCACAGCTCTTGGGTATGAATGTATGGAGTAAAGAGAAACACCTCTTTAAACACAAACAGCAAGTCAATGCGGT
GCAGTTCCTAGTTGGTAAAGTGACTGCTGACCAATCACACATGGGCATTGTGTACGCGGGTTACTACGCGGTTGATATGT
ACGATGCGCAAGGCAACAAAGTGTGGTCTGTGGCTAACGATGACCTGAACAGCGGCAAAATTGGTGTCTCCGCTTACGAC
TTTACCGGTGATGGTATTGATGAAGTGTTGGTTCAAGATCGTTTGAGAATGCGTATTCTCGATGGCCAAACGGGCCGTGT
GATGGGCATTATTGCGAACTCTTCTGGCACCTTGTGGGAATACCCAGTCGTGGCAGACTTAGAAGGCAACAATAACGCTT
CACTGATCATGGTTGCCAATGACTACGATCGCGAAAGCCAAGTCAACCACGGTGTGTTTGTGTATGAGTCCGCCAATCCA
AGCAAGCCTTGGCGAAATGCGACTCGTATCTGGAACCAGTACGCGTTTAATTTCTCAGATATTAACGCCAACGGTACTAT
CCCAACGAATGCACAACCAAGCTGGTTAACCCACAACAGCTTCCGTTCAGCGACCATTCGCGTTCCGCTGAAGTAA

Upstream 100 bases:

>100_bases
CCACCATCTCTTCCACGTATTTGCGCATCAATCTAACGAGAGAGTAAGGAAACTCATTATACAACCCACTCGAACTGTCA
AGAGAGATGAAGGTCAAGCC

Downstream 100 bases:

>100_bases
AGATACTTCTGCCAGCCGCTCCATCCGGAGCGGCTTTTTTATCGCTATGCTCACATCTTCTTCCACTCTTACGCCACAAA
GCCACTGATTTTCCACAAAT

Product: hemolysin-related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MKQTKTLTAISVLALSHLMTQSTAFASSSSDIQTKLKWSWSTSVFHPESNQVMAAPIVVQLNDDNGDGKIDEKDVADIIV
VTFEGNKYANGGYIRALSGVDGSELWSYSNGGVIADARYAPAAADLDGDGLIEIVSTSALTPYINILDHQGNIKKQLLKS
ASGWRSVGDIALADINGDGNIEILAADGVYSYESGLLFSHDWAPSSIAFDSNGDGQREVFANGTLYQNNGAYLWQYQAND
TVWFSSVANLDGDDKPELVVSVPASLSTPENSEIAVLEHDGSVKWRVNNLSNPGGSVQAVSSFLGKPSSSATTVDAQSAV
YGYTDWAHQQRVLAENHQLAIRSGAVVDAIGANSQNMIGGSGGSLSTIDTSKVRAIDVTYGKNKYTWKYGVLEMSFTLDN
GAKVTVGSKDSAFTYLGLEWKTKTVPYLGVEWRTKTVSYWFFGWHTKQVAYLAPVWKEKTIPYAVPVTLSKSTTVRYDIP
QGSQLLGMNVWSKEKHLFKHKQQVNAVQFLVGKVTADQSHMGIVYAGYYAVDMYDAQGNKVWSVANDDLNSGKIGVSAYD
FTGDGIDEVLVQDRLRMRILDGQTGRVMGIIANSSGTLWEYPVVADLEGNNNASLIMVANDYDRESQVNHGVFVYESANP
SKPWRNATRIWNQYAFNFSDINANGTIPTNAQPSWLTHNSFRSATIRVPLK

Sequences:

>Translated_691_residues
MKQTKTLTAISVLALSHLMTQSTAFASSSSDIQTKLKWSWSTSVFHPESNQVMAAPIVVQLNDDNGDGKIDEKDVADIIV
VTFEGNKYANGGYIRALSGVDGSELWSYSNGGVIADARYAPAAADLDGDGLIEIVSTSALTPYINILDHQGNIKKQLLKS
ASGWRSVGDIALADINGDGNIEILAADGVYSYESGLLFSHDWAPSSIAFDSNGDGQREVFANGTLYQNNGAYLWQYQAND
TVWFSSVANLDGDDKPELVVSVPASLSTPENSEIAVLEHDGSVKWRVNNLSNPGGSVQAVSSFLGKPSSSATTVDAQSAV
YGYTDWAHQQRVLAENHQLAIRSGAVVDAIGANSQNMIGGSGGSLSTIDTSKVRAIDVTYGKNKYTWKYGVLEMSFTLDN
GAKVTVGSKDSAFTYLGLEWKTKTVPYLGVEWRTKTVSYWFFGWHTKQVAYLAPVWKEKTIPYAVPVTLSKSTTVRYDIP
QGSQLLGMNVWSKEKHLFKHKQQVNAVQFLVGKVTADQSHMGIVYAGYYAVDMYDAQGNKVWSVANDDLNSGKIGVSAYD
FTGDGIDEVLVQDRLRMRILDGQTGRVMGIIANSSGTLWEYPVVADLEGNNNASLIMVANDYDRESQVNHGVFVYESANP
SKPWRNATRIWNQYAFNFSDINANGTIPTNAQPSWLTHNSFRSATIRVPLK
>Mature_691_residues
MKQTKTLTAISVLALSHLMTQSTAFASSSSDIQTKLKWSWSTSVFHPESNQVMAAPIVVQLNDDNGDGKIDEKDVADIIV
VTFEGNKYANGGYIRALSGVDGSELWSYSNGGVIADARYAPAAADLDGDGLIEIVSTSALTPYINILDHQGNIKKQLLKS
ASGWRSVGDIALADINGDGNIEILAADGVYSYESGLLFSHDWAPSSIAFDSNGDGQREVFANGTLYQNNGAYLWQYQAND
TVWFSSVANLDGDDKPELVVSVPASLSTPENSEIAVLEHDGSVKWRVNNLSNPGGSVQAVSSFLGKPSSSATTVDAQSAV
YGYTDWAHQQRVLAENHQLAIRSGAVVDAIGANSQNMIGGSGGSLSTIDTSKVRAIDVTYGKNKYTWKYGVLEMSFTLDN
GAKVTVGSKDSAFTYLGLEWKTKTVPYLGVEWRTKTVSYWFFGWHTKQVAYLAPVWKEKTIPYAVPVTLSKSTTVRYDIP
QGSQLLGMNVWSKEKHLFKHKQQVNAVQFLVGKVTADQSHMGIVYAGYYAVDMYDAQGNKVWSVANDDLNSGKIGVSAYD
FTGDGIDEVLVQDRLRMRILDGQTGRVMGIIANSSGTLWEYPVVADLEGNNNASLIMVANDYDRESQVNHGVFVYESANP
SKPWRNATRIWNQYAFNFSDINANGTIPTNAQPSWLTHNSFRSATIRVPLK

Specific function: Bacterial hemolysin that causes cytolysis by forming heptameric pores in target host membranes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein. Note=In the hemolytic biotype El Tor the 80 kDa hemolysin precursor is secreted as monomer. After binding to target membranes the protein assembles into a heptameric prepore complex. Proteolytic c

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ricin B-type lectin domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022220
- InterPro:   IPR001340
- InterPro:   IPR008997
- InterPro:   IPR000772 [H]

Pfam domain/function: PF12563 Hemolysin_N; PF07968 Leukocidin; PF00652 Ricin_B_lectin [H]

EC number: NA

Molecular weight: Translated: 75330; Mature: 75330

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTKTLTAISVLALSHLMTQSTAFASSSSDIQTKLKWSWSTSVFHPESNQVMAAPIVVQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCEEECCCCCCEEEEEEEEE
LNDDNGDGKIDEKDVADIIVVTFEGNKYANGGYIRALSGVDGSELWSYSNGGVIADARYA
EECCCCCCCCCCCCCEEEEEEEECCCEECCCCEEEEECCCCHHHHEEECCCCEEEECCCC
PAAADLDGDGLIEIVSTSALTPYINILDHQGNIKKQLLKSASGWRSVGDIALADINGDGN
CCCCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCEEEEEECCCCC
IEILAADGVYSYESGLLFSHDWAPSSIAFDSNGDGQREVFANGTLYQNNGAYLWQYQAND
EEEEECCCEEECCCCEEEEECCCCCEEEECCCCCCCEEEEECCEEEECCCEEEEEEECCC
TVWFSSVANLDGDDKPELVVSVPASLSTPENSEIAVLEHDGSVKWRVNNLSNPGGSVQAV
EEEEEEECCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCHHHH
SSFLGKPSSSATTVDAQSAVYGYTDWAHQQRVLAENHQLAIRSGAVVDAIGANSQNMIGG
HHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHCCCEEEEECCCEEEEECCCCCCEECC
SGGSLSTIDTSKVRAIDVTYGKNKYTWKYGVLEMSFTLDNGAKVTVGSKDSAFTYLGLEW
CCCCEEEEECCCEEEEEEEECCCEEEEEEEEEEEEEEECCCCEEEECCCCCEEEEEEEEE
KTKTVPYLGVEWRTKTVSYWFFGWHTKQVAYLAPVWKEKTIPYAVPVTLSKSTTVRYDIP
EECCCCEEEEEEEEEEEEEEEEEEECCEEEEEECCCCCCCCCEEEEEEECCCEEEEEECC
QGSQLLGMNVWSKEKHLFKHKQQVNAVQFLVGKVTADQSHMGIVYAGYYAVDMYDAQGNK
CCCEEEEECCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCCEEEEEEEEEEEEEECCCCE
VWSVANDDLNSGKIGVSAYDFTGDGIDEVLVQDRLRMRILDGQTGRVMGIIANSSGTLWE
EEEEECCCCCCCEEEEEEEECCCCCHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCEEE
YPVVADLEGNNNASLIMVANDYDRESQVNHGVFVYESANPSKPWRNATRIWNQYAFNFSD
CCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHEEEEEE
INANGTIPTNAQPSWLTHNSFRSATIRVPLK
ECCCCCCCCCCCCCEEECCCCCCEEEEEECC
>Mature Secondary Structure
MKQTKTLTAISVLALSHLMTQSTAFASSSSDIQTKLKWSWSTSVFHPESNQVMAAPIVVQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCEEECCCCCCEEEEEEEEE
LNDDNGDGKIDEKDVADIIVVTFEGNKYANGGYIRALSGVDGSELWSYSNGGVIADARYA
EECCCCCCCCCCCCCEEEEEEEECCCEECCCCEEEEECCCCHHHHEEECCCCEEEECCCC
PAAADLDGDGLIEIVSTSALTPYINILDHQGNIKKQLLKSASGWRSVGDIALADINGDGN
CCCCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCEEEEEECCCCC
IEILAADGVYSYESGLLFSHDWAPSSIAFDSNGDGQREVFANGTLYQNNGAYLWQYQAND
EEEEECCCEEECCCCEEEEECCCCCEEEECCCCCCCEEEEECCEEEECCCEEEEEEECCC
TVWFSSVANLDGDDKPELVVSVPASLSTPENSEIAVLEHDGSVKWRVNNLSNPGGSVQAV
EEEEEEECCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCHHHH
SSFLGKPSSSATTVDAQSAVYGYTDWAHQQRVLAENHQLAIRSGAVVDAIGANSQNMIGG
HHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHCCCEEEEECCCEEEEECCCCCCEECC
SGGSLSTIDTSKVRAIDVTYGKNKYTWKYGVLEMSFTLDNGAKVTVGSKDSAFTYLGLEW
CCCCEEEEECCCEEEEEEEECCCEEEEEEEEEEEEEEECCCCEEEECCCCCEEEEEEEEE
KTKTVPYLGVEWRTKTVSYWFFGWHTKQVAYLAPVWKEKTIPYAVPVTLSKSTTVRYDIP
EECCCCEEEEEEEEEEEEEEEEEEECCEEEEEECCCCCCCCCEEEEEEECCCEEEEEECC
QGSQLLGMNVWSKEKHLFKHKQQVNAVQFLVGKVTADQSHMGIVYAGYYAVDMYDAQGNK
CCCEEEEECCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCCEEEEEEEEEEEEEECCCCE
VWSVANDDLNSGKIGVSAYDFTGDGIDEVLVQDRLRMRILDGQTGRVMGIIANSSGTLWE
EEEEECCCCCCCEEEEEEEECCCCCHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCEEE
YPVVADLEGNNNASLIMVANDYDRESQVNHGVFVYESANPSKPWRNATRIWNQYAFNFSD
CCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHEEEEEE
INANGTIPTNAQPSWLTHNSFRSATIRVPLK
ECCCCCCCCCCCCCEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3050359; 2453464; 2174833; 10952301 [H]