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The map label for this gene is pflA [H]

Identifier: 227081998

GI number: 227081998

Start: 1949020

End: 1949760

Strand: Direct

Name: pflA [H]

Synonym: VCM66_1792

Alternate gene names: 227081998

Gene position: 1949020-1949760 (Clockwise)

Preceding gene: 227081996

Following gene: 227081999

Centisome position: 67.38

GC content: 46.15

Gene sequence:

>741_bases
ATGTCTACCATTGGTCGAATTCACTCTTTCGAATCTTGTGGCACTGTTGATGGCCCAGGAATCCGCTTTATTGTCTTCCT
GCAAGGGTGCCTGTTTCGCTGCAAGTATTGTCATAACCGAGACACTTGGGATACCCATACGGGTCGTGAAGTCACGGTTG
AGGAAATCATCAAAGAAGCGAAATCTTATCGCCATTTTATGAACGCTTCTGGTGGCGGCATCACCTGCTCTGGTGGGGAA
GCCATGCTGCAACCCGAATTTGTGCGTGACTTTTTCCGCGCAGCGAAAGCCGAAGGTATTCATACCTGTTTAGACACCAA
TGGCTACGTGCGTAAATTCACACCTGTGATTGACGAAGTGTTGGAAGTGACTGATTTGGTGATGCTCGATATCAAACAGA
TGGATGATGAGATCCACCAAGATTTGATTGGCGTGTCTAACAAACGCACCTTAGATTTTGCGCGCTACCTGCATCAAATT
GGTCAAAAAACTTGGTTGCGTTACGTGGTGGTTCCAGGTTACACCGATGATGAAGCATCTGCTCATCAACTGGGTGAGTT
TATTAAAGACATGGAAAACATCGAGAAAATTGAGCTGCTGCCTTACCACAAACTTGGCGCACACAAATGGGAAGCCATGG
GCGAAGAATATCCACTGGAAGGCGTGAATCCGCCAAGCAAAGAAACCATGGATAAAATCGTAGCGATCCTTGAGCAGTAT
CACTCAAACGTAAAATATTGA

Upstream 100 bases:

>100_bases
CTGTCACTGGTGATGCGACCAATTATCAGGGAGATAAACCTATTTAATCCCTGCTATTTGTAATAAAATAACGGCCAAAC
TACATAACTGAGAAGCGCTC

Downstream 100 bases:

>100_bases
TTCATCCTCTCGACGTTATCGTAAAAAATACCAACCTCAGGTTGGTATTTTTTTAGGTAACGCAATTGGAACTCGCACAA
ATCGCTTCGACCATCATTCT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSTIGRIHSFESCGTVDGPGIRFIVFLQGCLFRCKYCHNRDTWDTHTGREVTVEEIIKEAKSYRHFMNASGGGITCSGGE
AMLQPEFVRDFFRAAKAEGIHTCLDTNGYVRKFTPVIDEVLEVTDLVMLDIKQMDDEIHQDLIGVSNKRTLDFARYLHQI
GQKTWLRYVVVPGYTDDEASAHQLGEFIKDMENIEKIELLPYHKLGAHKWEAMGEEYPLEGVNPPSKETMDKIVAILEQY
HSNVKY

Sequences:

>Translated_246_residues
MSTIGRIHSFESCGTVDGPGIRFIVFLQGCLFRCKYCHNRDTWDTHTGREVTVEEIIKEAKSYRHFMNASGGGITCSGGE
AMLQPEFVRDFFRAAKAEGIHTCLDTNGYVRKFTPVIDEVLEVTDLVMLDIKQMDDEIHQDLIGVSNKRTLDFARYLHQI
GQKTWLRYVVVPGYTDDEASAHQLGEFIKDMENIEKIELLPYHKLGAHKWEAMGEEYPLEGVNPPSKETMDKIVAILEQY
HSNVKY
>Mature_245_residues
STIGRIHSFESCGTVDGPGIRFIVFLQGCLFRCKYCHNRDTWDTHTGREVTVEEIIKEAKSYRHFMNASGGGITCSGGEA
MLQPEFVRDFFRAAKAEGIHTCLDTNGYVRKFTPVIDEVLEVTDLVMLDIKQMDDEIHQDLIGVSNKRTLDFARYLHQIG
QKTWLRYVVVPGYTDDEASAHQLGEFIKDMENIEKIELLPYHKLGAHKWEAMGEEYPLEGVNPPSKETMDKIVAILEQYH
SNVKY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=73.5772357723577, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1790389, Length=255, Percent_Identity=26.6666666666667, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790839, Length=268, Percent_Identity=28.7313432835821, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI226510931, Length=270, Percent_Identity=27.037037037037, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28111; Mature: 27980

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCC
>Mature Secondary Structure 
STIGRIHSFESCGTVDGPGIRFIVFLQGCLFRCKYCHNRDTWDTHTGREVTVEEIIKEA
CCHHHHHCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
KSYRHFMNASGGGITCSGGEAMLQPEFVRDFFRAAKAEGIHTCLDTNGYVRKFTPVIDEV
HHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
LEVTDLVMLDIKQMDDEIHQDLIGVSNKRTLDFARYLHQIGQKTWLRYVVVPGYTDDEAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCHH
AHQLGEFIKDMENIEKIELLPYHKLGAHKWEAMGEEYPLEGVNPPSKETMDKIVAILEQY
HHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HSNVKY
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]