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The map label for this gene is xth [H]

Identifier: 227081989

GI number: 227081989

Start: 1936658

End: 1937464

Strand: Direct

Name: xth [H]

Synonym: VCM66_1783

Alternate gene names: 227081989

Gene position: 1936658-1937464 (Clockwise)

Preceding gene: 227081987

Following gene: 227081994

Centisome position: 66.95

GC content: 46.1

Gene sequence:

>807_bases
ATGAAAGTTATCAGCTTCAACATAAATGGACTCAGAGCCAGATTGCATCAACTGCAAGCGTTGATCGACAAGCATCAACC
TGATGTGATTGGCCTACAAGAGATCAAAGTCCATGATGAGGCGTTTCCTCGTCAAGAAGTCGAGGCCATGGGCTATCAAG
TCTATTTTCACGGTCAAAAAGCGCACTACGGTGTGGCCATTTTATGCAAACAAACGCCTGTCGAAGTGATCAAAGGCTTT
CCAACCGACAATGAAGAACACCAAAAGCGCATGATCATGGCGACATTCGCTGATCAGAATGGGCAGAAGACCACCATTCT
CAATGGCTATTTCCCACAAGGTGATAACGTCGAACACGAAACCAAGTTTCCTTACAAACGTCAGTTTTATCGTGATTTGA
TGACCTATCTGCGTGAACACCGCAGTAATTCAGAGCGTCTGGTCGTGATGGGTGACATCAATATCAGCCCACTCGATCTG
GATATTGGTATCGGCGAAGCCAACCGCAAACGCTGGTTACAAACAGGTAAATGTTCATTTCAACCAGAAGAGCGTGAGTG
GTTACAAACGCTTTTAGATTGGGGATTGGTCGATACTTTCCGCCAATTGCATCCTGACGTCAGTGACCAATTTTCGTGGT
TTGATTACCGCTCACGCGGCTTTGAAGACAACCGTGGACTTCGGATCGATGTGATTTTGGCAACACCAACACTGGCTGAA
ACTTGTCAAGAGGCTGGCATCGATTATGAGTTACGTGCGATCGATAAACCTTCCGATCATGCGCCTATCTGGACAACATT
CAAGTAA

Upstream 100 bases:

>100_bases
GCTTACTTTGTTGGTTCACTATTGAACCAATAATTCGCCGGACTTGAGATCCCGAGCTTAGGTGATAACATCAGTCCCAA
GTCAACCAAAAGGAATCGCA

Downstream 100 bases:

>100_bases
TCGAGTGCTCTGACGGCTTACTTAAAAATCCCCATCGACATTGATGGGGATTTTTTATTTAGCTGCGAGGTTTTAGGTAG
GCAAGAAAACGTTGTTCCGC

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKVISFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPRQEVEAMGYQVYFHGQKAHYGVAILCKQTPVEVIKGF
PTDNEEHQKRMIMATFADQNGQKTTILNGYFPQGDNVEHETKFPYKRQFYRDLMTYLREHRSNSERLVVMGDINISPLDL
DIGIGEANRKRWLQTGKCSFQPEEREWLQTLLDWGLVDTFRQLHPDVSDQFSWFDYRSRGFEDNRGLRIDVILATPTLAE
TCQEAGIDYELRAIDKPSDHAPIWTTFK

Sequences:

>Translated_268_residues
MKVISFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPRQEVEAMGYQVYFHGQKAHYGVAILCKQTPVEVIKGF
PTDNEEHQKRMIMATFADQNGQKTTILNGYFPQGDNVEHETKFPYKRQFYRDLMTYLREHRSNSERLVVMGDINISPLDL
DIGIGEANRKRWLQTGKCSFQPEEREWLQTLLDWGLVDTFRQLHPDVSDQFSWFDYRSRGFEDNRGLRIDVILATPTLAE
TCQEAGIDYELRAIDKPSDHAPIWTTFK
>Mature_268_residues
MKVISFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPRQEVEAMGYQVYFHGQKAHYGVAILCKQTPVEVIKGF
PTDNEEHQKRMIMATFADQNGQKTTILNGYFPQGDNVEHETKFPYKRQFYRDLMTYLREHRSNSERLVVMGDINISPLDL
DIGIGEANRKRWLQTGKCSFQPEEREWLQTLLDWGLVDTFRQLHPDVSDQFSWFDYRSRGFEDNRGLRIDVILATPTLAE
TCQEAGIDYELRAIDKPSDHAPIWTTFK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=25.5555555555556, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=25.5555555555556, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=25.5555555555556, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=62.6865671641791, Blast_Score=376, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=25.0922509225092, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221330655, Length=274, Percent_Identity=30.2919708029197, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17136678, Length=274, Percent_Identity=30.2919708029197, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31228; Mature: 31228

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVISFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPRQEVEAMGYQVYFHGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEECHHHEEECCHHCCHHHHHHCCEEEEEECCC
AHYGVAILCKQTPVEVIKGFPTDNEEHQKRMIMATFADQNGQKTTILNGYFPQGDNVEHE
CCCCEEEEECCCCHHHHCCCCCCCHHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCC
TKFPYKRQFYRDLMTYLREHRSNSERLVVMGDINISPLDLDIGIGEANRKRWLQTGKCSF
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHCCCCCC
QPEEREWLQTLLDWGLVDTFRQLHPDVSDQFSWFDYRSRGFEDNRGLRIDVILATPTLAE
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCHHHH
TCQEAGIDYELRAIDKPSDHAPIWTTFK
HHHHCCCCEEEEECCCCCCCCCEEEECC
>Mature Secondary Structure
MKVISFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPRQEVEAMGYQVYFHGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEECHHHEEECCHHCCHHHHHHCCEEEEEECCC
AHYGVAILCKQTPVEVIKGFPTDNEEHQKRMIMATFADQNGQKTTILNGYFPQGDNVEHE
CCCCEEEEECCCCHHHHCCCCCCCHHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCC
TKFPYKRQFYRDLMTYLREHRSNSERLVVMGDINISPLDLDIGIGEANRKRWLQTGKCSF
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHCCCCCC
QPEEREWLQTLLDWGLVDTFRQLHPDVSDQFSWFDYRSRGFEDNRGLRIDVILATPTLAE
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCHHHH
TCQEAGIDYELRAIDKPSDHAPIWTTFK
HHHHCCCCEEEEECCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]