Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 227081976

GI number: 227081976

Start: 1923057

End: 1923578

Strand: Reverse

Name: ruvC

Synonym: VCM66_1770

Alternate gene names: 227081976

Gene position: 1923578-1923057 (Counterclockwise)

Preceding gene: 227081977

Following gene: 227081975

Centisome position: 66.5

GC content: 49.62

Gene sequence:

>522_bases
ATGTCTGTCATCTTAGGGATTGATCCTGGATCGCGCGTGACGGGGTATGGTGTGATTCGTCAACAAGGACGACACCTTAT
CTACCTCGGTAGCGGTTGTATCCGTACTTCCGATCTCGAGTTACCGTTACGCCTGAAACAAATCTATGCGGGCGTGAGTG
AAATCATCACCCAATTTCAGCCGGATGCCTTTGCCATTGAGCAGGTGTTTATGGCAAAGAATGCCGATTCAGCATTAAAA
CTTGGTCAAGCCCGCGGCAGTGCGATTGTGGCGGCGGTGAACGCAGAACTTCCTGTTTATGAATATGCTGCCAGACTGAT
TAAACAAGCGGTCGTTGGTACGGGAGCGGCTGATAAAAGCCAAGTTCAACATATGGTGCAGCAGATGCTCAAACTGCCCG
GTAAGCCCCAAGCCGATGCAGCAGATGCTTTAGGTGTTGCTATTTGTCATGCTAATACCAACAAAACTCTAATTGCATTG
GCAGGGCAGGCAACGAGTGCGCGTAGAGGTCGTTATCGTTAA

Upstream 100 bases:

>100_bases
GCGTCGTAAGTAACGAGCAACAAATGAAAGGGTGGGCGTAAGCCCACTCTCTTTTTCTGGCACGTGTAGCCCATCTTTAA
ACGAACAAGGCAAGTGAGTT

Downstream 100 bases:

>100_bases
ATGTAGCTTGATGCTACGAGCATTTTACTGGCTATCCATCCAGTTATTGATTATCATCCAGAGCATCTTTCCTCCCTCTA
TTATAAGGAAACACAAGTGA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSVILGIDPGSRVTGYGVIRQQGRHLIYLGSGCIRTSDLELPLRLKQIYAGVSEIITQFQPDAFAIEQVFMAKNADSALK
LGQARGSAIVAAVNAELPVYEYAARLIKQAVVGTGAADKSQVQHMVQQMLKLPGKPQADAADALGVAICHANTNKTLIAL
AGQATSARRGRYR

Sequences:

>Translated_173_residues
MSVILGIDPGSRVTGYGVIRQQGRHLIYLGSGCIRTSDLELPLRLKQIYAGVSEIITQFQPDAFAIEQVFMAKNADSALK
LGQARGSAIVAAVNAELPVYEYAARLIKQAVVGTGAADKSQVQHMVQQMLKLPGKPQADAADALGVAICHANTNKTLIAL
AGQATSARRGRYR
>Mature_172_residues
SVILGIDPGSRVTGYGVIRQQGRHLIYLGSGCIRTSDLELPLRLKQIYAGVSEIITQFQPDAFAIEQVFMAKNADSALKL
GQARGSAIVAAVNAELPVYEYAARLIKQAVVGTGAADKSQVQHMVQQMLKLPGKPQADAADALGVAICHANTNKTLIALA
GQATSARRGRYR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=173, Percent_Identity=71.6763005780347, Blast_Score=230, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_VIBC3 (A5F762)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F762
- SMR:   A5F762
- STRING:   A5F762
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1438
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- BioCyc:   VCHO345073:VC0395_A1438-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18432; Mature: 18300

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVILGIDPGSRVTGYGVIRQQGRHLIYLGSGCIRTSDLELPLRLKQIYAGVSEIITQFQ
CEEEEECCCCCCEEHHHHHHHCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCC
PDAFAIEQVFMAKNADSALKLGQARGSAIVAAVNAELPVYEYAARLIKQAVVGTGAADKS
CCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHH
QVQHMVQQMLKLPGKPQADAADALGVAICHANTNKTLIALAGQATSARRGRYR
HHHHHHHHHHHCCCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SVILGIDPGSRVTGYGVIRQQGRHLIYLGSGCIRTSDLELPLRLKQIYAGVSEIITQFQ
EEEEECCCCCCEEHHHHHHHCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCC
PDAFAIEQVFMAKNADSALKLGQARGSAIVAAVNAELPVYEYAARLIKQAVVGTGAADKS
CCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHH
QVQHMVQQMLKLPGKPQADAADALGVAICHANTNKTLIALAGQATSARRGRYR
HHHHHHHHHHHCCCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA