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The map label for this gene is ruvB [H]

Identifier: 227081974

GI number: 227081974

Start: 1921286

End: 1922290

Strand: Reverse

Name: ruvB [H]

Synonym: VCM66_1768

Alternate gene names: 227081974

Gene position: 1922290-1921286 (Counterclockwise)

Preceding gene: 227081975

Following gene: 227081973

Centisome position: 66.46

GC content: 47.16

Gene sequence:

>1005_bases
ATGATAGAAGCCGATCGTTTAATTGCTCCAATCAGCAACCACTTCAAAGATGAGGAAGTGATTGATCGAGCGATTCGTCC
CAAAAAGCTTGCCGATTACCAAGGTCAAGATCATGTTCGTGATCAGATGGAAATCTTCATTCAAGCGGCACAAATGCGCC
AAGAAGCCTTAGATCATCTGCTGATTTTCGGGCCTCCGGGCTTGGGTAAAACCACCTTGGCCAACATAGTCGCTAACGAA
ATGGGCGTGAATATTCGTACTACTTCAGGTCCTGTATTGGAAAAAGCGGGAGATTTAGCGGCACTGCTAACCAATCTTGA
AGAAAACGATGTGCTGTTTATTGATGAAATCCATCGTCTGAGCCCAATGGTTGAGGAAGTACTGTATCCCGCTATGGAAG
ATTACCAACTCGATATCATGATTGGTGAAGGCCCAGCTGCACGCTCAATCAAAATCGATCTCCCTCCTTTTACTTTAATT
GGAGCCACCACACGTGCAGGCTCGCTGACTTCACCACTGCGTGACCGATTTGGGATTGTGCAACGATTGGAGTATTACAA
AGTCGCCGATTTACAACACATAGTGCAACGTAGTGCACAGTGTCTCGGTCTTTCGATGGATAGTGAGGGCGCTCTTGAAG
TGGCTCGCCGTGCACGTGGAACCCCACGGATTGCCAATCGCCTGCTGCGCCGGGTACGAGATTATGCCGAAGTAAAAGGG
GATGGGCACATTTGTGCGCAAACCGCTGACAGGGCACTCAATATGCTTGATGTTGACCATCAAGGCTTTGATTACATGGA
TAGAAAGCTGCTGTTAGCAATAATGGAAAAGTTTTCAGGTGGCCCAGTCGGAATCGATAACCTTGCGGCAGCTATTGGCG
AAGAGAAAGATACGATAGAAGATGTTCTTGAGCCATTTCTCATTCAGCAGGGTTATTTACAACGTACACCACGCGGTCGT
ATCGCGACAGATCGCGCTTATTTGCATTTCGGTATTGAAAAATAA

Upstream 100 bases:

>100_bases
AGCAGTGAAGAGTTGATTCGTGAAGCGCTCAAATCTATGGTGTAACGATTCACGTTTTGCCGTCGTTAAAAACAGTCGCC
ATTAAGCCAATAGAGAAAAT

Downstream 100 bases:

>100_bases
TGTTACAAATCTCGCTTGTTAAACCGAGCGAGATACTCTTTTTTGACAAAGTTCTCATTTCTTGTATTTCCTTTCCTCGC
TCAATTGTTGGATTTTTTGG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MIEADRLIAPISNHFKDEEVIDRAIRPKKLADYQGQDHVRDQMEIFIQAAQMRQEALDHLLIFGPPGLGKTTLANIVANE
MGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGIVQRLEYYKVADLQHIVQRSAQCLGLSMDSEGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICAQTADRALNMLDVDHQGFDYMDRKLLLAIMEKFSGGPVGIDNLAAAIGEEKDTIEDVLEPFLIQQGYLQRTPRGR
IATDRAYLHFGIEK

Sequences:

>Translated_334_residues
MIEADRLIAPISNHFKDEEVIDRAIRPKKLADYQGQDHVRDQMEIFIQAAQMRQEALDHLLIFGPPGLGKTTLANIVANE
MGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGIVQRLEYYKVADLQHIVQRSAQCLGLSMDSEGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICAQTADRALNMLDVDHQGFDYMDRKLLLAIMEKFSGGPVGIDNLAAAIGEEKDTIEDVLEPFLIQQGYLQRTPRGR
IATDRAYLHFGIEK
>Mature_334_residues
MIEADRLIAPISNHFKDEEVIDRAIRPKKLADYQGQDHVRDQMEIFIQAAQMRQEALDHLLIFGPPGLGKTTLANIVANE
MGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGIVQRLEYYKVADLQHIVQRSAQCLGLSMDSEGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICAQTADRALNMLDVDHQGFDYMDRKLLLAIMEKFSGGPVGIDNLAAAIGEEKDTIEDVLEPFLIQQGYLQRTPRGR
IATDRAYLHFGIEK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=332, Percent_Identity=80.421686746988, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37299; Mature: 37299

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEADRLIAPISNHFKDEEVIDRAIRPKKLADYQGQDHVRDQMEIFIQAAQMRQEALDHL
CCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
LIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
SPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIV
HHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHH
QRLEYYKVADLQHIVQRSAQCLGLSMDSEGALEVARRARGTPRIANRLLRRVRDYAEVKG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DGHICAQTADRALNMLDVDHQGFDYMDRKLLLAIMEKFSGGPVGIDNLAAAIGEEKDTIE
CCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
DVLEPFLIQQGYLQRTPRGRIATDRAYLHFGIEK
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MIEADRLIAPISNHFKDEEVIDRAIRPKKLADYQGQDHVRDQMEIFIQAAQMRQEALDHL
CCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
LIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
SPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIV
HHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHH
QRLEYYKVADLQHIVQRSAQCLGLSMDSEGALEVARRARGTPRIANRLLRRVRDYAEVKG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DGHICAQTADRALNMLDVDHQGFDYMDRKLLLAIMEKFSGGPVGIDNLAAAIGEEKDTIE
CCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
DVLEPFLIQQGYLQRTPRGRIATDRAYLHFGIEK
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA