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The map label for this gene is frwABC [H]

Identifier: 227081951

GI number: 227081951

Start: 1899418

End: 1901283

Strand: Reverse

Name: frwABC [H]

Synonym: VCM66_1745

Alternate gene names: 227081951

Gene position: 1901283-1899418 (Counterclockwise)

Preceding gene: 227081954

Following gene: 227081947

Centisome position: 65.73

GC content: 49.46

Gene sequence:

>1866_bases
GTGGATATTACCCATCTGATCGAACCTGAAATCATCTGTTTAGATCTAAAAGCCAATAACAAAGAAGATGTTCTGATTGA
GCTGGTTGAGTTGCTCGATAACGCAGGAAAGTTGACCGATAAGCAGCAATTTTTAAACGACATTTGGCGACGAGAAGAGA
TCGGAAATACAGGGTTTGAAGAAGGAATAGCCATTCCACACGCCAAAAGTGCTGCGGTGGCGTTGCCTGCAGTCGTCGTG
GGTATTAGCCGCCAAGGGATCGATTACGGTGCAGAAGATGGTCAGCTTTCTGATGTGTTTTTTATGCTGGCATCACCGGA
TGGAGAAGATCATCACCACATTGAAGTATTGGCACAAATCTCCAGTAAATTGATTGAAGAGGGATTTGTTGAACAGCTCA
AAGCCGCAGAAAGTGTTGAGCAGGCACTCGCCCTTTTTATTCACCCACAGCACTCAACATTAAGCTCTACACAGGTAATG
GATTATGCCGATTACCAGCCGCTAAGCCCAATGGCACAAAGATTAGCTCGCGTTAAAGAGCATCTATTATTTGGTACCTC
TCACATGATGCCGTTTATCGTGGCGGGTGGGGTATTACTCTCGCTTTCAGTGATGATGTCTGGTCACGGAGCGGTGCCAG
AAGAGGGCGTGTTGGCGGATATCGCTCAAATGGGTATTGCCGGTTTAACCCTATTTACGGTGGTATTGGGTGGTTATATC
GCTTACTCGATGGCAGATAAACCCGGTTTGGCCCCCGACATGATTGGCACCTGGATTGCGATAAACCAATATCAAACCGG
CTTTATTGGCGCGATCATCGTGGGTTTTTTTGCAGGCTTTGTGGTTCGTCAGCTGAAAAGAATCGCTCTCCCCGACAGCA
TGAGTTCGCTGGGTTCCATCTTCATCTATCCGCTGGTGGGGACGTTTGTAACCTGTGGTGCTGTGATGTGGTTAATCGGT
GCACCGATTGCCAGCAGTATGCTGTGGCTCAATCAATTTTTGGCATCGATGGCCGATTCCGGCAAAGTGGTGCTCGGAGC
GGTATTGGGGGCGATGACTGCCTTTGACATGGGCGGCCCAATCAATAAAGTCGCCACGCTGTTTGCCCAAACTCAAGTCA
ACACCCAACCTTGGCTAATGGGTGGGGTGGGGATTGCCATTTGTACCCCACCATTGGGGATGGCGCTTGCGACCTTATTG
TCACCAAGTAAATTCAAACGGGATGAACGTGAAGCCGGAAAAGCGGCAGGCATCATGGGTATGATTGGCATTAGTGAAGG
GGCGATTCCGTTTGCCGCCGCTGATCCCGCGCGCGTACTCCCTGCAATTATTGCGGGTGGTATTGTTGGCAACGTGATTG
GTTTTCTCTTTCAAGTGCTCAATCATGCCCCTTGGGGCGGTTGGATTGTGCTGCCCGTTGTGGACGGCAAGTTAGGGTAT
ATTCTTGGCACTATCGCCGGTGCATTAGCCACGGCACTGATTGCTATTGCCCTGAAGAAAACGGTGCATGAGCAGGACAA
TGAGCAGGGGCAGTCACTTGCTTTTTCATCGGTTATTGGAGAAGGGCAAGCCGACATTCTTGCCGTCACCTCGTGCCCTT
CTGGCGTTGCTCATACGTTTTTAGCGGCTAAATCACTTGAAAAAGCCGCCTGTCTGGCGGGAGTGAAAATCAAAGTAGAA
ACACAAGGCGCCAACGGTATCATCAATCGAATCACGGCCAAAGATGTGCAACGAGCGAAGCTGGTGATCTTTGCGCACGA
TGTGGCGATTAAAGAACCAGAACGCTTCAAGCACATTAAAGTCATTGACGTGACAACGAAAGACGCGATTTTAAATGCCG
CGGCATTGGTGCAGATAAAGCGATAA

Upstream 100 bases:

>100_bases
GCATCTTTTGTCCTGTGTCTTGAAAGAGTATCGCTCGTTACTCTAGCGAGGCGCATCGGTATGGCAATGCCAATTCAAAC
AACAGTAAGGTAAAACAATT

Downstream 100 bases:

>100_bases
CATTCTGAATCACTACACTTGAGATAGAAAATGAGCCCTTTGGGGGAAAGGGCTCTGGGGGAGGAGCTTAAGGTTTAAGC
TTGGATAAGTTCGGCGGCTT

Product: PTS system, fructose-specific IIABC component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system fructose-like EIIC component 2 [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MDITHLIEPEIICLDLKANNKEDVLIELVELLDNAGKLTDKQQFLNDIWRREEIGNTGFEEGIAIPHAKSAAVALPAVVV
GISRQGIDYGAEDGQLSDVFFMLASPDGEDHHHIEVLAQISSKLIEEGFVEQLKAAESVEQALALFIHPQHSTLSSTQVM
DYADYQPLSPMAQRLARVKEHLLFGTSHMMPFIVAGGVLLSLSVMMSGHGAVPEEGVLADIAQMGIAGLTLFTVVLGGYI
AYSMADKPGLAPDMIGTWIAINQYQTGFIGAIIVGFFAGFVVRQLKRIALPDSMSSLGSIFIYPLVGTFVTCGAVMWLIG
APIASSMLWLNQFLASMADSGKVVLGAVLGAMTAFDMGGPINKVATLFAQTQVNTQPWLMGGVGIAICTPPLGMALATLL
SPSKFKRDEREAGKAAGIMGMIGISEGAIPFAAADPARVLPAIIAGGIVGNVIGFLFQVLNHAPWGGWIVLPVVDGKLGY
ILGTIAGALATALIAIALKKTVHEQDNEQGQSLAFSSVIGEGQADILAVTSCPSGVAHTFLAAKSLEKAACLAGVKIKVE
TQGANGIINRITAKDVQRAKLVIFAHDVAIKEPERFKHIKVIDVTTKDAILNAAALVQIKR

Sequences:

>Translated_621_residues
MDITHLIEPEIICLDLKANNKEDVLIELVELLDNAGKLTDKQQFLNDIWRREEIGNTGFEEGIAIPHAKSAAVALPAVVV
GISRQGIDYGAEDGQLSDVFFMLASPDGEDHHHIEVLAQISSKLIEEGFVEQLKAAESVEQALALFIHPQHSTLSSTQVM
DYADYQPLSPMAQRLARVKEHLLFGTSHMMPFIVAGGVLLSLSVMMSGHGAVPEEGVLADIAQMGIAGLTLFTVVLGGYI
AYSMADKPGLAPDMIGTWIAINQYQTGFIGAIIVGFFAGFVVRQLKRIALPDSMSSLGSIFIYPLVGTFVTCGAVMWLIG
APIASSMLWLNQFLASMADSGKVVLGAVLGAMTAFDMGGPINKVATLFAQTQVNTQPWLMGGVGIAICTPPLGMALATLL
SPSKFKRDEREAGKAAGIMGMIGISEGAIPFAAADPARVLPAIIAGGIVGNVIGFLFQVLNHAPWGGWIVLPVVDGKLGY
ILGTIAGALATALIAIALKKTVHEQDNEQGQSLAFSSVIGEGQADILAVTSCPSGVAHTFLAAKSLEKAACLAGVKIKVE
TQGANGIINRITAKDVQRAKLVIFAHDVAIKEPERFKHIKVIDVTTKDAILNAAALVQIKR
>Mature_621_residues
MDITHLIEPEIICLDLKANNKEDVLIELVELLDNAGKLTDKQQFLNDIWRREEIGNTGFEEGIAIPHAKSAAVALPAVVV
GISRQGIDYGAEDGQLSDVFFMLASPDGEDHHHIEVLAQISSKLIEEGFVEQLKAAESVEQALALFIHPQHSTLSSTQVM
DYADYQPLSPMAQRLARVKEHLLFGTSHMMPFIVAGGVLLSLSVMMSGHGAVPEEGVLADIAQMGIAGLTLFTVVLGGYI
AYSMADKPGLAPDMIGTWIAINQYQTGFIGAIIVGFFAGFVVRQLKRIALPDSMSSLGSIFIYPLVGTFVTCGAVMWLIG
APIASSMLWLNQFLASMADSGKVVLGAVLGAMTAFDMGGPINKVATLFAQTQVNTQPWLMGGVGIAICTPPLGMALATLL
SPSKFKRDEREAGKAAGIMGMIGISEGAIPFAAADPARVLPAIIAGGIVGNVIGFLFQVLNHAPWGGWIVLPVVDGKLGY
ILGTIAGALATALIAIALKKTVHEQDNEQGQSLAFSSVIGEGQADILAVTSCPSGVAHTFLAAKSLEKAACLAGVKIKVE
TQGANGIINRITAKDVQRAKLVIFAHDVAIKEPERFKHIKVIDVTTKDAILNAAALVQIKR

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790386, Length=318, Percent_Identity=46.2264150943396, Blast_Score=274, Evalue=2e-74,
Organism=Escherichia coli, GI1788492, Length=329, Percent_Identity=42.5531914893617, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1786951, Length=348, Percent_Identity=31.3218390804598, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082348, Length=294, Percent_Identity=31.9727891156463, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788729, Length=378, Percent_Identity=25.9259259259259, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788730, Length=93, Percent_Identity=46.2365591397849, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790387, Length=92, Percent_Identity=48.9130434782609, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI2367327, Length=146, Percent_Identity=33.5616438356164, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790390, Length=82, Percent_Identity=40.2439024390244, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788726, Length=145, Percent_Identity=29.6551724137931, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013014
- InterPro:   IPR006327 [H]

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 65755; Mature: 65755

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITHLIEPEIICLDLKANNKEDVLIELVELLDNAGKLTDKQQFLNDIWRREEIGNTGFE
CCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
EGIAIPHAKSAAVALPAVVVGISRQGIDYGAEDGQLSDVFFMLASPDGEDHHHIEVLAQI
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
SSKLIEEGFVEQLKAAESVEQALALFIHPQHSTLSSTQVMDYADYQPLSPMAQRLARVKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHH
HLLFGTSHMMPFIVAGGVLLSLSVMMSGHGAVPEEGVLADIAQMGIAGLTLFTVVLGGYI
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYSMADKPGLAPDMIGTWIAINQYQTGFIGAIIVGFFAGFVVRQLKRIALPDSMSSLGSI
HHHCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FIYPLVGTFVTCGAVMWLIGAPIASSMLWLNQFLASMADSGKVVLGAVLGAMTAFDMGGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
INKVATLFAQTQVNTQPWLMGGVGIAICTPPLGMALATLLSPSKFKRDEREAGKAAGIMG
HHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHCCHHHCCHHHHHHHHHCCEE
MIGISEGAIPFAAADPARVLPAIIAGGIVGNVIGFLFQVLNHAPWGGWIVLPVVDGKLGY
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHH
ILGTIAGALATALIAIALKKTVHEQDNEQGQSLAFSSVIGEGQADILAVTSCPSGVAHTF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
LAAKSLEKAACLAGVKIKVETQGANGIINRITAKDVQRAKLVIFAHDVAIKEPERFKHIK
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCHHHCCEE
VIDVTTKDAILNAAALVQIKR
EEEECHHHHHHHHHHHEEECC
>Mature Secondary Structure
MDITHLIEPEIICLDLKANNKEDVLIELVELLDNAGKLTDKQQFLNDIWRREEIGNTGFE
CCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
EGIAIPHAKSAAVALPAVVVGISRQGIDYGAEDGQLSDVFFMLASPDGEDHHHIEVLAQI
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
SSKLIEEGFVEQLKAAESVEQALALFIHPQHSTLSSTQVMDYADYQPLSPMAQRLARVKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHH
HLLFGTSHMMPFIVAGGVLLSLSVMMSGHGAVPEEGVLADIAQMGIAGLTLFTVVLGGYI
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYSMADKPGLAPDMIGTWIAINQYQTGFIGAIIVGFFAGFVVRQLKRIALPDSMSSLGSI
HHHCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FIYPLVGTFVTCGAVMWLIGAPIASSMLWLNQFLASMADSGKVVLGAVLGAMTAFDMGGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
INKVATLFAQTQVNTQPWLMGGVGIAICTPPLGMALATLLSPSKFKRDEREAGKAAGIMG
HHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHCCHHHCCHHHHHHHHHCCEE
MIGISEGAIPFAAADPARVLPAIIAGGIVGNVIGFLFQVLNHAPWGGWIVLPVVDGKLGY
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHH
ILGTIAGALATALIAIALKKTVHEQDNEQGQSLAFSSVIGEGQADILAVTSCPSGVAHTF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
LAAKSLEKAACLAGVKIKVETQGANGIINRITAKDVQRAKLVIFAHDVAIKEPERFKHIK
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCHHHCCEE
VIDVTTKDAILNAAALVQIKR
EEEECHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]