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The map label for this gene is pspA [H]

Identifier: 227081829

GI number: 227081829

Start: 1758941

End: 1759609

Strand: Reverse

Name: pspA [H]

Synonym: VCM66_1618

Alternate gene names: 227081829

Gene position: 1759609-1758941 (Counterclockwise)

Preceding gene: 227081837

Following gene: 227081828

Centisome position: 60.83

GC content: 46.79

Gene sequence:

>669_bases
ATGGGTATTTTTTCTCGTTTTGCAGACATCGTAAATTCCAACATCAGCGCGCTACTGGACAAAGCAGAAGATCCAGAAAA
AATGATCCGCCTGATTATTCAGGAGATGGAAGATACGTTAGTGGAAGTGCGTACCAATTCCGCCAAAGCAATGGCAGATA
AAAAAGAGCTGGCGCGTAAAGTGGAGTCGATTGAACAGCAGTTAGAGGATTGGCAGAACAAAGCGACATTGGCGCTGACT
AAACAACGTGAAGATTTGGCGCGCGCGGCACTGATCGAGAAGCAGAAACTGCAACATGTGTTGAAAGGTTTGCACACAGA
GCAGATGCTTGTCGAAGAAACCATTGAAAAGCTAACAGGCGAAATTGGTAAGCTCGAAACCAAAATCACTGAAACTCGTG
CGAAACAGCAAGCGTTAGCGATTCGTAGCCAAGCGGCGAGTCATCGTCGCGATGTGCAGCGTCATTTGCACGCGGGTCGT
ACCGAAGAAGCGATGGCAAAGTTTGAGCAGTTCTCGCGTAAAGTCGATGAGCTGGAAGCGGAAGCCGATCTTTATGCACA
ATCAGGCCAAGCGCGCTCTTTAGAGCAAGAATTTGCTGAGCTACAAGCACAAGATGAGATTGAAAAAGAGCTCAACAAGC
TTAAAGCTCAGATGAAATCAGACCAATAA

Upstream 100 bases:

>100_bases
AAAGTTGGCACGTAATTTGGATTTATCTTTGCAACCAACGCACAGCATGGGGCTGCAGCGGTAATCTCAGTGTTTGGCAC
TGAAGGATAGGAGAAACGTC

Downstream 100 bases:

>100_bases
TTGGGAGTAGACTATGTCCTCATTTTTTATCGCTGTGCCATTGATCGTTTTTTGTATCTTTGTTGCACCGCTGTGGCTGA
TCCTCCACTACCGGAGTAAA

Product: phage shock protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARKVESIEQQLEDWQNKATLALT
KQREDLARAALIEKQKLQHVLKGLHTEQMLVEETIEKLTGEIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGR
TEEAMAKFEQFSRKVDELEAEADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKAQMKSDQ

Sequences:

>Translated_222_residues
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARKVESIEQQLEDWQNKATLALT
KQREDLARAALIEKQKLQHVLKGLHTEQMLVEETIEKLTGEIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGR
TEEAMAKFEQFSRKVDELEAEADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKAQMKSDQ
>Mature_221_residues
GIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARKVESIEQQLEDWQNKATLALTK
QREDLARAALIEKQKLQHVLKGLHTEQMLVEETIEKLTGEIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGRT
EEAMAKFEQFSRKVDELEAEADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKAQMKSDQ

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=221, Percent_Identity=58.3710407239819, Blast_Score=254, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25382; Mature: 25251

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARK
CCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VESIEQQLEDWQNKATLALTKQREDLARAALIEKQKLQHVLKGLHTEQMLVEETIEKLTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGRTEEAMAKFEQFSRKVDELEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKAQMKSDQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARK
CHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VESIEQQLEDWQNKATLALTKQREDLARAALIEKQKLQHVLKGLHTEQMLVEETIEKLTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGRTEEAMAKFEQFSRKVDELEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKAQMKSDQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]