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The map label for this gene is ttgG [H]

Identifier: 227081825

GI number: 227081825

Start: 1756028

End: 1757137

Strand: Reverse

Name: ttgG [H]

Synonym: VCM66_1614

Alternate gene names: 227081825

Gene position: 1757137-1756028 (Counterclockwise)

Preceding gene: 227081826

Following gene: 227081824

Centisome position: 60.75

GC content: 49.64

Gene sequence:

>1110_bases
GTGAATGGGTCAAGGAGCGAGGGCTGTAGGATGAAAATAATTCGTTACACCGCTTGGTTGGCTCACTTACTGTTGATCGC
AGGATGCAGTGAACCCACGAAGCTCGAACGCGATACGCTACCGCGAGTAAAGGTGGCGCAGGCAGGTGAAAAAGTCAGTG
ATAAGCTCTTTTTTCCTGCGGTGGCCAATGCGGCTGAACGTTCTCATTTGAGTTTTCGAGTGGCAGGTGAAATCAGTAAG
TTCCATGTCAAAGAGGGCGCTCGCGTTAAGCAAGGCGACATCTTGGCTGAAATTGAGCCGACCGATTACCGTTTGGCTGT
CGATAATGCTCAAGCACGATTTTCCGTGATTGATAGCCAATATCGGCGTTCACAACCACTTGTCGAAAAAGGCTTATTAG
CCAAATCACAGTTTGACGAAATTGCTGCGCAGCGCCAAATCGCTCGTGCTGAGCTTGAGTTGGCGAAACTTCGTCTCTCC
TTCACCCAATTACGCGCACCAATGGATGGCATCATCTCGCGTGTCAGCGCTGAGCAGTTTGAAAGTGTTCAGGTTGGCCA
ACAAATCGTCAATATTCACAATATCGATAGCGTTGAAGTTTTGATTCAACTGCCTGATCGCCTGTATGCGACACAACCGA
CGGCCAGCGAGTTGGCGAAGATAGAAACCGTGGTGCGTGTCCCAAGTGGTCAAGTTTATCCCGCACGTATCAAAGAATTC
ACCACTGAGCCAGACCCTGCCACGGGGACATTTACTGTCACCCTTTCTCTGCCCATGCCAGAGCAAGAACTGATCTTGGA
TGGCATGGCTGTTGAAGTGAGTGCCAAAGGGCAACATGTCGGGCTAAACCCACTCAATACCATCAGTATTCCGATTGAGG
CGATTTTTAATGCAGACGGTGATGAGTTAGACAAAACCGCTCAATACGTTTGGTTGCTCAACAACGACAATACGGTGAGC
AAACGTCAGGTGCGGTTAGCGAAAGCGTCACGCAGCCATGTTCAAGTCAGTGAAGGAATTAACGCGGGAGATCGTTTAGT
GGTCGCTGGCGTTGCAAGATTGAGAGATGGAATGAAAGTACAAGTCCTTGAGGAGCGTGGCCATGAGTGA

Upstream 100 bases:

>100_bases
GTCACTTTGAATGAACAACGCCAAGTGCTTACAGGCTTAAATGATGGCGACCGGATCATTATTTCCGGTGTCAGTGGCGT
GACCGAGGGCATAAAAGTGC

Downstream 100 bases:

>100_bases
ACAGAAGCAGACCCCGCCAGAGAATAATGAGAACCAAGGTGTTGCCGCTTACTTCATCAATAATCGCGTGATCAGTTGGA
TGGTTTCGCTGATTTTTTTG

Product: putative periplasmic linker protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNGSRSEGCRMKIIRYTAWLAHLLLIAGCSEPTKLERDTLPRVKVAQAGEKVSDKLFFPAVANAAERSHLSFRVAGEISK
FHVKEGARVKQGDILAEIEPTDYRLAVDNAQARFSVIDSQYRRSQPLVEKGLLAKSQFDEIAAQRQIARAELELAKLRLS
FTQLRAPMDGIISRVSAEQFESVQVGQQIVNIHNIDSVEVLIQLPDRLYATQPTASELAKIETVVRVPSGQVYPARIKEF
TTEPDPATGTFTVTLSLPMPEQELILDGMAVEVSAKGQHVGLNPLNTISIPIEAIFNADGDELDKTAQYVWLLNNDNTVS
KRQVRLAKASRSHVQVSEGINAGDRLVVAGVARLRDGMKVQVLEERGHE

Sequences:

>Translated_369_residues
MNGSRSEGCRMKIIRYTAWLAHLLLIAGCSEPTKLERDTLPRVKVAQAGEKVSDKLFFPAVANAAERSHLSFRVAGEISK
FHVKEGARVKQGDILAEIEPTDYRLAVDNAQARFSVIDSQYRRSQPLVEKGLLAKSQFDEIAAQRQIARAELELAKLRLS
FTQLRAPMDGIISRVSAEQFESVQVGQQIVNIHNIDSVEVLIQLPDRLYATQPTASELAKIETVVRVPSGQVYPARIKEF
TTEPDPATGTFTVTLSLPMPEQELILDGMAVEVSAKGQHVGLNPLNTISIPIEAIFNADGDELDKTAQYVWLLNNDNTVS
KRQVRLAKASRSHVQVSEGINAGDRLVVAGVARLRDGMKVQVLEERGHE
>Mature_369_residues
MNGSRSEGCRMKIIRYTAWLAHLLLIAGCSEPTKLERDTLPRVKVAQAGEKVSDKLFFPAVANAAERSHLSFRVAGEISK
FHVKEGARVKQGDILAEIEPTDYRLAVDNAQARFSVIDSQYRRSQPLVEKGLLAKSQFDEIAAQRQIARAELELAKLRLS
FTQLRAPMDGIISRVSAEQFESVQVGQQIVNIHNIDSVEVLIQLPDRLYATQPTASELAKIETVVRVPSGQVYPARIKEF
TTEPDPATGTFTVTLSLPMPEQELILDGMAVEVSAKGQHVGLNPLNTISIPIEAIFNADGDELDKTAQYVWLLNNDNTVS
KRQVRLAKASRSHVQVSEGINAGDRLVVAGVARLRDGMKVQVLEERGHE

Specific function: The periplasmic linker component of an organic solvent efflux pump. Involved in export of a number of organic solvents, including toluene and styrene. This is the most important solvent efflux pump in this strain, although it can export AMP and some antib

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=331, Percent_Identity=27.7945619335347, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786668, Length=333, Percent_Identity=26.1261261261261, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=24.2587601078167, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789929, Length=336, Percent_Identity=25.8928571428571, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081796, Length=325, Percent_Identity=23.3846153846154, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40753; Mature: 40753

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGSRSEGCRMKIIRYTAWLAHLLLIAGCSEPTKLERDTLPRVKVAQAGEKVSDKLFFPA
CCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHCCCHHHHCEECHH
VANAAERSHLSFRVAGEISKFHVKEGARVKQGDILAEIEPTDYRLAVDNAQARFSVIDSQ
HHHHHHHCCEEEEEECCCCEEECCCCCEEECCCEEEEECCCCEEEEECCCCHHHHHHHHH
YRRSQPLVEKGLLAKSQFDEIAAQRQIARAELELAKLRLSFTQLRAPMDGIISRVSAEQF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ESVQVGQQIVNIHNIDSVEVLIQLPDRLYATQPTASELAKIETVVRVPSGQVYPARIKEF
HHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCCCHHHHHHHHHHEECCCCCCCHHHHHHC
TTEPDPATGTFTVTLSLPMPEQELILDGMAVEVSAKGQHVGLNPLNTISIPIEAIFNADG
CCCCCCCCCEEEEEEECCCCHHHHHHCCEEEEEECCCCEECCCCCCEEEEEEEEEECCCC
DELDKTAQYVWLLNNDNTVSKRQVRLAKASRSHVQVSEGINAGDRLVVAGVARLRDGMKV
HHHHCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEHHCCCCCCCEEEEEEHHHHCCCCEE
QVLEERGHE
EEECCCCCC
>Mature Secondary Structure
MNGSRSEGCRMKIIRYTAWLAHLLLIAGCSEPTKLERDTLPRVKVAQAGEKVSDKLFFPA
CCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHCCCHHHHCEECHH
VANAAERSHLSFRVAGEISKFHVKEGARVKQGDILAEIEPTDYRLAVDNAQARFSVIDSQ
HHHHHHHCCEEEEEECCCCEEECCCCCEEECCCEEEEECCCCEEEEECCCCHHHHHHHHH
YRRSQPLVEKGLLAKSQFDEIAAQRQIARAELELAKLRLSFTQLRAPMDGIISRVSAEQF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ESVQVGQQIVNIHNIDSVEVLIQLPDRLYATQPTASELAKIETVVRVPSGQVYPARIKEF
HHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCCCHHHHHHHHHHEECCCCCCCHHHHHHC
TTEPDPATGTFTVTLSLPMPEQELILDGMAVEVSAKGQHVGLNPLNTISIPIEAIFNADG
CCCCCCCCCEEEEEEECCCCHHHHHHCCEEEEEECCCCEECCCCCCEEEEEEEEEECCCC
DELDKTAQYVWLLNNDNTVSKRQVRLAKASRSHVQVSEGINAGDRLVVAGVARLRDGMKV
HHHHCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEHHCCCCCCCEEEEEEHHHHCCCCEE
QVLEERGHE
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11395460 [H]