BacMap

Definition	Vibrio cholerae M66-2 chromosome I, complete genome.
Accession	NC_012578
Length	2,892,523

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The map label for this gene is ynjD [H]

Identifier: 227081817

GI number: 227081817

Start: 1745415

End: 1746077

Strand: Direct

Name: ynjD [H]

Synonym: VCM66_1606

Alternate gene names: 227081817

Gene position: 1745415-1746077 (Clockwise)

Preceding gene: 227081816

Following gene: 227081818

Centisome position: 60.34

GC content: 48.72

Gene sequence:

>663_bases
ATGATATTTTCTCTCGAAAACCTCGCCATCCTTAAACAAGATGGCCACTGCCTTTTGGCAGAGTTTAATTTGACTATCGC
AGAAGGCGAGATTGTGAGCCTAATGGGCCCTAGTGGCTGCGGCAAATCCACCTTACTGAGCGTGATTGCGGGTCATCTCG
CCACGGATTTTACGTTTCAAGGTTCGATGAAACTTGGGGATACGCAGCTCAACACCTTACCGGCTCATCAACGCCAAGTA
GGCATGCTTTTTCAAGACGATTGGCTTTTTCCACACCTCAATGTGTGGGAAAACTTAGCGTTTGCGTTGCCGAATTCGAT
TAAAGGCGAACAACGTAAGAAAAACGCTTATGCCGCTTTAACGAAAATTGCCCTCACTGAGCTGGCTTGCTCGTTTCCCG
AGCAGATCTCTGGTGGCCAACGGGCACGGATCAGTTTGATGCGCATGCTGCTCGCCGAGCCCAAATTGGTTCTGCTGGAT
GAACCCTTCAGTAAATTAGATAAAGCCTTACGCAGCCAATTTCGAGATTGGGTTTTCAATCAACTGACCAGCGCAGGGAT
CCCAACACTCATGGTCACTCATGATCATGAGGATGTGCCAGTGGGCAGTCGTGTCATTGAATGGCCCGTCATTGAGCAAG
AAACAGAGGTAAACCATGCTTGA

Upstream 100 bases:

>100_bases
TGCCAAGCACTCCTGCCTTTCGTGTTCTTTTCAATAGCTCTGCTTCTCGGACGTATGCAAATTCACCGCTACCGAGCAGC
ACAATTGACAACGGTATTTC

Downstream 100 bases:

>100_bases
TCGCTACGCGATAAAAATCATTCGCTGGCCACTTCATCAAGCCGCAGGATTGCTGGATGAAGCGGGCGTCACCGCCAATC
AAACCACGGTATTCGGCTTT

Product: ABC transporter, ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MIFSLENLAILKQDGHCLLAEFNLTIAEGEIVSLMGPSGCGKSTLLSVIAGHLATDFTFQGSMKLGDTQLNTLPAHQRQV
GMLFQDDWLFPHLNVWENLAFALPNSIKGEQRKKNAYAALTKIALTELACSFPEQISGGQRARISLMRMLLAEPKLVLLD
EPFSKLDKALRSQFRDWVFNQLTSAGIPTLMVTHDHEDVPVGSRVIEWPVIEQETEVNHA

Sequences:

>Translated_220_residues
MIFSLENLAILKQDGHCLLAEFNLTIAEGEIVSLMGPSGCGKSTLLSVIAGHLATDFTFQGSMKLGDTQLNTLPAHQRQV
GMLFQDDWLFPHLNVWENLAFALPNSIKGEQRKKNAYAALTKIALTELACSFPEQISGGQRARISLMRMLLAEPKLVLLD
EPFSKLDKALRSQFRDWVFNQLTSAGIPTLMVTHDHEDVPVGSRVIEWPVIEQETEVNHA
>Mature_220_residues
MIFSLENLAILKQDGHCLLAEFNLTIAEGEIVSLMGPSGCGKSTLLSVIAGHLATDFTFQGSMKLGDTQLNTLPAHQRQV
GMLFQDDWLFPHLNVWENLAFALPNSIKGEQRKKNAYAALTKIALTELACSFPEQISGGQRARISLMRMLLAEPKLVLLD
EPFSKLDKALRSQFRDWVFNQLTSAGIPTLMVTHDHEDVPVGSRVIEWPVIEQETEVNHA

Specific function: Probably part of a binding-protein-dependent transport system YnjCD. Probably responsible for energy coupling to the transport system [H]

COG id: COG4136

COG function: function code R; ABC-type uncharacterized transport system, ATPase component

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI46592956, Length=164, Percent_Identity=34.7560975609756, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI46592971, Length=164, Percent_Identity=34.7560975609756, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI46592978, Length=164, Percent_Identity=34.7560975609756, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI46592915, Length=164, Percent_Identity=34.7560975609756, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI46592898, Length=164, Percent_Identity=34.7560975609756, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI46592964, Length=164, Percent_Identity=34.7560975609756, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI215820660, Length=155, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI20143975, Length=155, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI11967971, Length=166, Percent_Identity=33.1325301204819, Blast_Score=74, Evalue=7e-14,
Organism=Homo sapiens, GI9961252, Length=158, Percent_Identity=31.0126582278481, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4505771, Length=158, Percent_Identity=31.0126582278481, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI9961250, Length=158, Percent_Identity=31.0126582278481, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI153792144, Length=156, Percent_Identity=29.4871794871795, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI171184400, Length=165, Percent_Identity=32.7272727272727, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI105990541, Length=204, Percent_Identity=28.4313725490196, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI11967969, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI27262624, Length=186, Percent_Identity=28.494623655914, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI27262626, Length=186, Percent_Identity=28.494623655914, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI156105685, Length=201, Percent_Identity=30.8457711442786, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1788053, Length=190, Percent_Identity=48.9473684210526, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI87081791, Length=186, Percent_Identity=39.7849462365591, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1790467, Length=177, Percent_Identity=41.8079096045198, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1788761, Length=213, Percent_Identity=36.6197183098592, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787370, Length=186, Percent_Identity=37.6344086021505, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786253, Length=184, Percent_Identity=39.1304347826087, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87081709, Length=184, Percent_Identity=35.3260869565217, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1786563, Length=185, Percent_Identity=38.3783783783784, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787712, Length=185, Percent_Identity=37.8378378378378, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787029, Length=188, Percent_Identity=37.7659574468085, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI87082267, Length=196, Percent_Identity=33.6734693877551, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789032, Length=206, Percent_Identity=32.0388349514563, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1789672, Length=201, Percent_Identity=35.8208955223881, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788225, Length=205, Percent_Identity=34.1463414634146, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789873, Length=171, Percent_Identity=36.2573099415205, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1789593, Length=186, Percent_Identity=30.6451612903226, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1786703, Length=187, Percent_Identity=32.620320855615, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1786872, Length=166, Percent_Identity=34.9397590361446, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787164, Length=192, Percent_Identity=35.4166666666667, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787089, Length=209, Percent_Identity=33.9712918660287, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787105, Length=197, Percent_Identity=29.9492385786802, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1789962, Length=183, Percent_Identity=32.2404371584699, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1789586, Length=191, Percent_Identity=29.3193717277487, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788506, Length=200, Percent_Identity=33, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786698, Length=209, Percent_Identity=30.622009569378, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788450, Length=180, Percent_Identity=32.2222222222222, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081834, Length=219, Percent_Identity=28.7671232876712, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786398, Length=187, Percent_Identity=28.8770053475936, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790544, Length=172, Percent_Identity=33.1395348837209, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787500, Length=182, Percent_Identity=32.4175824175824, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787758, Length=182, Percent_Identity=29.1208791208791, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1789891, Length=194, Percent_Identity=32.4742268041237, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787792, Length=191, Percent_Identity=29.3193717277487, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994883, Length=188, Percent_Identity=27.1276595744681, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787547, Length=180, Percent_Identity=27.2222222222222, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693107, Length=178, Percent_Identity=31.4606741573034, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081782, Length=158, Percent_Identity=32.2784810126582, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1790162, Length=161, Percent_Identity=34.1614906832298, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1790739, Length=175, Percent_Identity=30.2857142857143, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788644, Length=139, Percent_Identity=33.0935251798561, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788897, Length=202, Percent_Identity=26.7326732673267, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787759, Length=194, Percent_Identity=28.3505154639175, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789963, Length=193, Percent_Identity=24.3523316062176, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786653, Length=178, Percent_Identity=28.0898876404494, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787143, Length=158, Percent_Identity=32.2784810126582, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790525, Length=192, Percent_Identity=27.6041666666667, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787182, Length=172, Percent_Identity=29.6511627906977, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789864, Length=208, Percent_Identity=25, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787113, Length=220, Percent_Identity=30.4545454545455, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193211017, Length=206, Percent_Identity=31.0679611650485, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71996809, Length=206, Percent_Identity=31.0679611650485, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI193211015, Length=206, Percent_Identity=31.0679611650485, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25146777, Length=161, Percent_Identity=33.5403726708075, Blast_Score=80, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI212646699, Length=202, Percent_Identity=28.7128712871287, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17510237, Length=194, Percent_Identity=28.3505154639175, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17541710, Length=160, Percent_Identity=32.5, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17569143, Length=159, Percent_Identity=31.4465408805031, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=214, Percent_Identity=27.5700934579439, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17569145, Length=159, Percent_Identity=32.7044025157233, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71992285, Length=173, Percent_Identity=27.7456647398844, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115532730, Length=155, Percent_Identity=30.9677419354839, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI115535101, Length=198, Percent_Identity=28.2828282828283, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115533592, Length=169, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=192, Percent_Identity=28.6458333333333, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17567265, Length=198, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567269, Length=198, Percent_Identity=28.7878787878788, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323217, Length=207, Percent_Identity=29.4685990338164, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319856, Length=187, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324498, Length=162, Percent_Identity=30.8641975308642, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI85726357, Length=199, Percent_Identity=30.6532663316583, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281363262, Length=210, Percent_Identity=30.4761904761905, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653245, Length=210, Percent_Identity=29.5238095238095, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28574533, Length=169, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI85724864, Length=162, Percent_Identity=28.3950617283951, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24580555, Length=162, Percent_Identity=28.3950617283951, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI85816269, Length=162, Percent_Identity=28.3950617283951, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24580553, Length=162, Percent_Identity=28.3950617283951, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI85725262, Length=198, Percent_Identity=28.7878787878788, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574744, Length=150, Percent_Identity=30, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582014, Length=198, Percent_Identity=26.7676767676768, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582012, Length=198, Percent_Identity=26.7676767676768, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136662, Length=186, Percent_Identity=29.5698924731183, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581191, Length=151, Percent_Identity=30.4635761589404, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581189, Length=151, Percent_Identity=30.4635761589404, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581193, Length=151, Percent_Identity=30.4635761589404, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581195, Length=151, Percent_Identity=30.4635761589404, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI161076682, Length=146, Percent_Identity=32.1917808219178, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076684, Length=146, Percent_Identity=32.1917808219178, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581617, Length=146, Percent_Identity=32.1917808219178, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581615, Length=146, Percent_Identity=32.1917808219178, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24666092, Length=181, Percent_Identity=29.8342541436464, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24659289, Length=157, Percent_Identity=31.8471337579618, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19920532, Length=166, Percent_Identity=31.3253012048193, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24580930, Length=166, Percent_Identity=31.3253012048193, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007184, Length=198, Percent_Identity=28.2828282828283, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221500365, Length=198, Percent_Identity=28.2828282828283, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 24454; Mature: 24454

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSLENLAILKQDGHCLLAEFNLTIAEGEIVSLMGPSGCGKSTLLSVIAGHLATDFTFQ
CEEECCCEEEEECCCCEEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCEEEE
GSMKLGDTQLNTLPAHQRQVGMLFQDDWLFPHLNVWENLAFALPNSIKGEQRKKNAYAAL
CCCEECCCCCCCCCCHHHHHCCEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
TKIALTELACSFPEQISGGQRARISLMRMLLAEPKLVLLDEPFSKLDKALRSQFRDWVFN
HHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHH
QLTSAGIPTLMVTHDHEDVPVGSRVIEWPVIEQETEVNHA
HHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MIFSLENLAILKQDGHCLLAEFNLTIAEGEIVSLMGPSGCGKSTLLSVIAGHLATDFTFQ
CEEECCCEEEEECCCCEEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCEEEE
GSMKLGDTQLNTLPAHQRQVGMLFQDDWLFPHLNVWENLAFALPNSIKGEQRKKNAYAAL
CCCEECCCCCCCCCCHHHHHCCEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
TKIALTELACSFPEQISGGQRARISLMRMLLAEPKLVLLDEPFSKLDKALRSQFRDWVFN
HHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHH
QLTSAGIPTLMVTHDHEDVPVGSRVIEWPVIEQETEVNHA
HHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]