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The map label for this gene is hlyB [H]

Identifier: 227081795

GI number: 227081795

Start: 1715474

End: 1717264

Strand: Direct

Name: hlyB [H]

Synonym: VCM66_1582

Alternate gene names: 227081795

Gene position: 1715474-1717264 (Clockwise)

Preceding gene: 227081792

Following gene: 227081796

Centisome position: 59.31

GC content: 47.29

Gene sequence:

>1791_bases
ATGAAATTCAATCCAATTATCCAGTTTGGACCATACTCAAATCAGTATAATTTCATTAACTTTTTACTTTTTGTCGGGGG
ATGGATGATGGTATCCAATCAACATAACAATGCTCATCGCACAATAATAGCGGGCACTACAAACTCACTGCATAGCGTTG
GCCTACGCTTGAAACTGATCGTGGTGCTGATCGTGGTTTCCATTCTTTTCTTGGGTTTCAAAGGCTTGACAGGCATGCAA
AGTGCTTCAGATTCGATTGAATCGCTTTATACAAAAGGAATGCAACATACGATTCGTGCTGGACGCATATTGGATGAGTT
AGGCAGCGCGCGCAGCCAACTGCTGTTAGCTTTCCAGCATGACCCTTCCTCCAAATACGCGCTGATGCATGATCATCCGA
TCACAATGCACGTCGAAGGTATCCAAACATCGCTGACCAATTTGCACCGTATTGTGGATAACGAAATATTGGCTACCGTA
TTGGATGTTCAAGAAAAAGCCGTGATCCAAACCTTGAAAGAAAAGCTGGATCAAGTCACTCGTCAAGGATTTGAACCCGC
TCTCCAACAGCTCAAGGCTGAAAAATACGATGCGGCTAACTTAATTCTGCTCCAAGTCATTAACCCTCTTTATAACGAGA
TCAAAAAAGCGGCGGAAGCCTTTCTCGACATTCAAACCCAAGAAGGAAAAAATAACTTTTCTGCCTCTCAACACGAGATT
AACCAATTTATCTGGATGGTCAGCTTACTGGGTGCTTTTGCCTTGATAGTGACTACCAGCTTAGCACTACTGATTGCTAA
GCGAGTCAATAATGCAGTGCACCACTTAGCCAGTTCTGCCACCCATATTGCCGCTGGCGATCTCACTCAACGCATCCCCG
TCACTGGGAACGATGAATTTTCGGATATTGCTAAATACGTCAACCGAATTGTAACGAGCTTTCAAGAAGTCATTAGCAGT
AATCGCCACTCAGTCTCTGCTTTAGCGACGGCTGCCGAGGAAAACGCGGCCGTTGCGATGCAAACGAAGCAAAACATCGT
CGAACAGCAGTCGCAAACACAGCAAATTGCTGCCGCAATCCATCAGTTCACCGCCACCGTACACGAAGTCGCGCAAAGTG
CAGGCCTTGCTGCAGAAGCCTCTGAAGAAGCAGAACAAGCCGCGATGCAGGGACGCAAGGTCGTTGAGGAAAATATTGCG
ATGATTGAAGGGCTATCCAACGATTTACAAAAGATGCTTGCCGCCATGCAGTTACTTGCCAAAGATTCAGAAGACATTGG
CAGTGTTGTGGATGTGATTCAGAGTATTTCAGAGCAAACCAATTTATTGGCGCTGAATGCTGCGATCGAAGCCGCTCGCG
CGGGTGAACAAGGACGAGGATTTGCAGTCGTCGCCGATGAGGTCAGAACCTTAGCAAGCCGCACCCAAGAATCGACGAAA
CAGATCCTACAGACAGTGCAGCGCTTACAGCAGAGCAGTCGAGATTCGGCGAATTTAATTGAACAAGGGGTTTCTGGAGC
ATCAAAAGCCGTTGAAAAAGCACGTTTGGCAGGGACGGCGCTGTCTCAAATTACGGCTAACGTCGATCGTATCTCGACCA
TGAACACGCAAATTGCGACAGCGTCAGAGGAGCAAAGTGCGGTGACTGAGGAGATCAATAAAAACATCATCACCATCAGC
GAAATCTCCAACCAAACCGCGTTGGGAGCACAACAAAGCTCTGAAGCAACCTTGGAGCTGGCAAGACTCGCCGAGAGCAT
GCAACAGGAAGTCGCGCGTTACAATGCCTGA

Upstream 100 bases:

>100_bases
TTTATAACAGTGGGTTCATTTTCAGTGTCTGATATTGTCAGTAGACCGTCATCCTTGTGAATATCATCCATAAAAATCAT
GATCTACGTATCATAACGTT

Downstream 100 bases:

>100_bases
CATATCCCAAAACGACTTGGAGTGGCAGCGAAACGAAAAACGAGTCTATCCTCATCAACATCTCAAACGATGTGATTAGG
ATGAGCGAACGTGCGAACAC

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKFNPIIQFGPYSNQYNFINFLLFVGGWMMVSNQHNNAHRTIIAGTTNSLHSVGLRLKLIVVLIVVSILFLGFKGLTGMQ
SASDSIESLYTKGMQHTIRAGRILDELGSARSQLLLAFQHDPSSKYALMHDHPITMHVEGIQTSLTNLHRIVDNEILATV
LDVQEKAVIQTLKEKLDQVTRQGFEPALQQLKAEKYDAANLILLQVINPLYNEIKKAAEAFLDIQTQEGKNNFSASQHEI
NQFIWMVSLLGAFALIVTTSLALLIAKRVNNAVHHLASSATHIAAGDLTQRIPVTGNDEFSDIAKYVNRIVTSFQEVISS
NRHSVSALATAAEENAAVAMQTKQNIVEQQSQTQQIAAAIHQFTATVHEVAQSAGLAAEASEEAEQAAMQGRKVVEENIA
MIEGLSNDLQKMLAAMQLLAKDSEDIGSVVDVIQSISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLASRTQESTK
QILQTVQRLQQSSRDSANLIEQGVSGASKAVEKARLAGTALSQITANVDRISTMNTQIATASEEQSAVTEEINKNIITIS
EISNQTALGAQQSSEATLELARLAESMQQEVARYNA

Sequences:

>Translated_596_residues
MKFNPIIQFGPYSNQYNFINFLLFVGGWMMVSNQHNNAHRTIIAGTTNSLHSVGLRLKLIVVLIVVSILFLGFKGLTGMQ
SASDSIESLYTKGMQHTIRAGRILDELGSARSQLLLAFQHDPSSKYALMHDHPITMHVEGIQTSLTNLHRIVDNEILATV
LDVQEKAVIQTLKEKLDQVTRQGFEPALQQLKAEKYDAANLILLQVINPLYNEIKKAAEAFLDIQTQEGKNNFSASQHEI
NQFIWMVSLLGAFALIVTTSLALLIAKRVNNAVHHLASSATHIAAGDLTQRIPVTGNDEFSDIAKYVNRIVTSFQEVISS
NRHSVSALATAAEENAAVAMQTKQNIVEQQSQTQQIAAAIHQFTATVHEVAQSAGLAAEASEEAEQAAMQGRKVVEENIA
MIEGLSNDLQKMLAAMQLLAKDSEDIGSVVDVIQSISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLASRTQESTK
QILQTVQRLQQSSRDSANLIEQGVSGASKAVEKARLAGTALSQITANVDRISTMNTQIATASEEQSAVTEEINKNIITIS
EISNQTALGAQQSSEATLELARLAESMQQEVARYNA
>Mature_596_residues
MKFNPIIQFGPYSNQYNFINFLLFVGGWMMVSNQHNNAHRTIIAGTTNSLHSVGLRLKLIVVLIVVSILFLGFKGLTGMQ
SASDSIESLYTKGMQHTIRAGRILDELGSARSQLLLAFQHDPSSKYALMHDHPITMHVEGIQTSLTNLHRIVDNEILATV
LDVQEKAVIQTLKEKLDQVTRQGFEPALQQLKAEKYDAANLILLQVINPLYNEIKKAAEAFLDIQTQEGKNNFSASQHEI
NQFIWMVSLLGAFALIVTTSLALLIAKRVNNAVHHLASSATHIAAGDLTQRIPVTGNDEFSDIAKYVNRIVTSFQEVISS
NRHSVSALATAAEENAAVAMQTKQNIVEQQSQTQQIAAAIHQFTATVHEVAQSAGLAAEASEEAEQAAMQGRKVVEENIA
MIEGLSNDLQKMLAAMQLLAKDSEDIGSVVDVIQSISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLASRTQESTK
QILQTVQRLQQSSRDSANLIEQGVSGASKAVEKARLAGTALSQITANVDRISTMNTQIATASEEQSAVTEEINKNIITIS
EISNQTALGAQQSSEATLELARLAESMQQEVARYNA

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=436, Percent_Identity=29.1284403669725, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI2367378, Length=337, Percent_Identity=30.2670623145401, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788194, Length=395, Percent_Identity=32.4050632911392, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788195, Length=285, Percent_Identity=32.9824561403509, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789453, Length=335, Percent_Identity=27.1641791044776, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 64831; Mature: 64831

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNPIIQFGPYSNQYNFINFLLFVGGWMMVSNQHNNAHRTIIAGTTNSLHSVGLRLKLI
CCCCCCEEECCCCCCHHHHHHHHHHCCHHEEECCCCCCCEEEEECCCCHHHHHHHHHHHH
VVLIVVSILFLGFKGLTGMQSASDSIESLYTKGMQHTIRAGRILDELGSARSQLLLAFQH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
DPSSKYALMHDHPITMHVEGIQTSLTNLHRIVDNEILATVLDVQEKAVIQTLKEKLDQVT
CCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQGFEPALQQLKAEKYDAANLILLQVINPLYNEIKKAAEAFLDIQTQEGKNNFSASQHEI
HHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
NQFIWMVSLLGAFALIVTTSLALLIAKRVNNAVHHLASSATHIAAGDLTQRIPVTGNDEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHCCCCCCCCCCH
SDIAKYVNRIVTSFQEVISSNRHSVSALATAAEENAAVAMQTKQNIVEQQSQTQQIAAAI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
HQFTATVHEVAQSAGLAAEASEEAEQAAMQGRKVVEENIAMIEGLSNDLQKMLAAMQLLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDSEDIGSVVDVIQSISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLASRTQESTK
CCCHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
QILQTVQRLQQSSRDSANLIEQGVSGASKAVEKARLAGTALSQITANVDRISTMNTQIAT
HHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
ASEEQSAVTEEINKNIITISEISNQTALGAQQSSEATLELARLAESMQQEVARYNA
CCHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFNPIIQFGPYSNQYNFINFLLFVGGWMMVSNQHNNAHRTIIAGTTNSLHSVGLRLKLI
CCCCCCEEECCCCCCHHHHHHHHHHCCHHEEECCCCCCCEEEEECCCCHHHHHHHHHHHH
VVLIVVSILFLGFKGLTGMQSASDSIESLYTKGMQHTIRAGRILDELGSARSQLLLAFQH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
DPSSKYALMHDHPITMHVEGIQTSLTNLHRIVDNEILATVLDVQEKAVIQTLKEKLDQVT
CCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQGFEPALQQLKAEKYDAANLILLQVINPLYNEIKKAAEAFLDIQTQEGKNNFSASQHEI
HHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
NQFIWMVSLLGAFALIVTTSLALLIAKRVNNAVHHLASSATHIAAGDLTQRIPVTGNDEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHCCCCCCCCCCH
SDIAKYVNRIVTSFQEVISSNRHSVSALATAAEENAAVAMQTKQNIVEQQSQTQQIAAAI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
HQFTATVHEVAQSAGLAAEASEEAEQAAMQGRKVVEENIAMIEGLSNDLQKMLAAMQLLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDSEDIGSVVDVIQSISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLASRTQESTK
CCCHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
QILQTVQRLQQSSRDSANLIEQGVSGASKAVEKARLAGTALSQITANVDRISTMNTQIAT
HHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
ASEEQSAVTEEINKNIITISEISNQTALGAQQSSEATLELARLAESMQQEVARYNA
CCHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]