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The map label for this gene is alsS [H]

Identifier: 227081743

GI number: 227081743

Start: 1648802

End: 1650505

Strand: Direct

Name: alsS [H]

Synonym: VCM66_1530

Alternate gene names: 227081743

Gene position: 1648802-1650505 (Clockwise)

Preceding gene: 227081742

Following gene: 227081744

Centisome position: 57.0

GC content: 51.88

Gene sequence:

>1704_bases
ATGCAATCCAACATTCACTTGCGCAGCGGAGCACAACTTATCGCCCAACAGCTTGAAGCTATTGGTGTGCGTTATGTCTT
CGGCATTCCGGGGGCCAAAATCGACCGTCTTTTCGACGCGATTGAGGATACCAAAATCCAAATGATCCCAGTCCGTCATG
AAGCGAATGGCGCTTTTATGGCGGGTATTATGGGGCGTCTTTCAGGTCGAGCGGGAGTCACCTTAGCCACATCGGGCCCC
GGCTGCGGCAACCTTGTAACAGGCGTCGCGACAGCCAATTCTGAAGGCGATCCGATGATCGCTATTGGCGGCGCAGTCAA
ACGTGACTATCAACAAAAGCAGACCCACCAGAGCATGGATACGGTGAGTATTTTTCGTTCTATCACCAAATACAGCGCCG
AAATTCAACATGTCGATGCCACCAGCGAAGTCATGGCTAATGCATTTCGTCTCGCCGAATCGGGTCGCCAAGGCGCGTGT
TTTATCAGCGTACCGCAAGATGTGCTGGCCGATAAAACCTCAAGCGAAATTATTATCCCCAGTGCTTATCAGCCCACGGC
TGAAGCCGACCCAGACAGTTTGCAGGAAGCCGTAGAACGGATTCAAAAAGCGCAGCGCTGCGTCGTATTACTCGGACTGC
ATGCCAGCCACCAAGCCACTGCTGAAGCGGTCAGCGCTTTTTTACATAAAACCCAGTTACCTGTCGTGGGCACCTACCAA
GCCGCTGGCGCAGTAGACATCAACTACTATCACCATTTCGCCGGACGAGTCGGCTTATTCAATAATCAGCCCGGAGATGT
GCTGCTTAAAGAGGCCGATTTGATCATCACCATCGGTTTTAATCCAATCGAGTACGATCCTGAGCTATGGAATAGCCAGC
GCTGTCCTATCGTTCATATCGATATTGAGCCAGCGCAGTATCAAATCCACTACCAGCCATGCCTACAAATTGTCGGTAAC
ATTGCCAGTAATTTGGCGGCACTCTGCCGCCCACTACCCGGTTTTACTCGCCTCTCCCCGACTGCATTAGCGATTTTGGA
AGAAGTGACTCAGCAGCGTCACGCCATCAAAGCCTATCCTTTGCGCTATCAAAATGCGGGATTTCATCCGCTAACCTTGA
TTAAAACCATGCAAGCGATCATCAAACCCGATACCACCCTCTGTCTCGATATGGGGAGTTTCCATATATGGATTGCGCGC
TATCTGAGCTGTTTTCGTGCACGGCAGATGCTGGTATCTAACGGACAGCAGACCATGGGCGTTGCCCTACCTTGGGCGAT
TGCGGCCAGCTTACTCAAACCCGGCCATAAAGTCGTTTCAGTATCCGGTGATGGCGGCTTTATGCAATCCAGCATGGAGC
TCGAAACCGCGGTGCGCCTCAAATGCAATATCGTACACATCATTTGGGTCGATAACGCTTACAACATGGTCGAGATGCAG
GAAGTGCAGAAATACCATCGCGCTGCTGGGGTAAAATTCGGCCCTATTGATTTTAAAGCCTACGCCGAATCCTTTGGCGC
TCGAGGCTACGCCATCACGCAACCCGATCAGTTGCTCCCTACTCTGCGCCAAGCGATGGAAGTCGAGGGCCCCGCCGTCG
TCGCGATTCCAGTCGATTACTCGGATAACCCCAAATTGATGCAAGCGCGCACCGAAGCCAATCAAGATCCCGTATTTCAA
ACCCTAGAAGGAGTTTTCGCATGA

Upstream 100 bases:

>100_bases
CGCTTTCCTGTTGCAATGATCTCTGTGCAGGAAGTGCCCACCCACCTATCGCAAAACAACTTTCACTTAGGGTGTAACGT
CAGGTAACTAAGGATCACTT

Downstream 100 bases:

>100_bases
GAGGTTTAACCCATAAAGTCGCCTTAGTGACAGGTGCCGCTAACGGTATTGGACTGGCGATTGCCGAACGTTTATATCAA
GAAGGCGCAACCTTAGCGCT

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MQSNIHLRSGAQLIAQQLEAIGVRYVFGIPGAKIDRLFDAIEDTKIQMIPVRHEANGAFMAGIMGRLSGRAGVTLATSGP
GCGNLVTGVATANSEGDPMIAIGGAVKRDYQQKQTHQSMDTVSIFRSITKYSAEIQHVDATSEVMANAFRLAESGRQGAC
FISVPQDVLADKTSSEIIIPSAYQPTAEADPDSLQEAVERIQKAQRCVVLLGLHASHQATAEAVSAFLHKTQLPVVGTYQ
AAGAVDINYYHHFAGRVGLFNNQPGDVLLKEADLIITIGFNPIEYDPELWNSQRCPIVHIDIEPAQYQIHYQPCLQIVGN
IASNLAALCRPLPGFTRLSPTALAILEEVTQQRHAIKAYPLRYQNAGFHPLTLIKTMQAIIKPDTTLCLDMGSFHIWIAR
YLSCFRARQMLVSNGQQTMGVALPWAIAASLLKPGHKVVSVSGDGGFMQSSMELETAVRLKCNIVHIIWVDNAYNMVEMQ
EVQKYHRAAGVKFGPIDFKAYAESFGARGYAITQPDQLLPTLRQAMEVEGPAVVAIPVDYSDNPKLMQARTEANQDPVFQ
TLEGVFA

Sequences:

>Translated_567_residues
MQSNIHLRSGAQLIAQQLEAIGVRYVFGIPGAKIDRLFDAIEDTKIQMIPVRHEANGAFMAGIMGRLSGRAGVTLATSGP
GCGNLVTGVATANSEGDPMIAIGGAVKRDYQQKQTHQSMDTVSIFRSITKYSAEIQHVDATSEVMANAFRLAESGRQGAC
FISVPQDVLADKTSSEIIIPSAYQPTAEADPDSLQEAVERIQKAQRCVVLLGLHASHQATAEAVSAFLHKTQLPVVGTYQ
AAGAVDINYYHHFAGRVGLFNNQPGDVLLKEADLIITIGFNPIEYDPELWNSQRCPIVHIDIEPAQYQIHYQPCLQIVGN
IASNLAALCRPLPGFTRLSPTALAILEEVTQQRHAIKAYPLRYQNAGFHPLTLIKTMQAIIKPDTTLCLDMGSFHIWIAR
YLSCFRARQMLVSNGQQTMGVALPWAIAASLLKPGHKVVSVSGDGGFMQSSMELETAVRLKCNIVHIIWVDNAYNMVEMQ
EVQKYHRAAGVKFGPIDFKAYAESFGARGYAITQPDQLLPTLRQAMEVEGPAVVAIPVDYSDNPKLMQARTEANQDPVFQ
TLEGVFA
>Mature_567_residues
MQSNIHLRSGAQLIAQQLEAIGVRYVFGIPGAKIDRLFDAIEDTKIQMIPVRHEANGAFMAGIMGRLSGRAGVTLATSGP
GCGNLVTGVATANSEGDPMIAIGGAVKRDYQQKQTHQSMDTVSIFRSITKYSAEIQHVDATSEVMANAFRLAESGRQGAC
FISVPQDVLADKTSSEIIIPSAYQPTAEADPDSLQEAVERIQKAQRCVVLLGLHASHQATAEAVSAFLHKTQLPVVGTYQ
AAGAVDINYYHHFAGRVGLFNNQPGDVLLKEADLIITIGFNPIEYDPELWNSQRCPIVHIDIEPAQYQIHYQPCLQIVGN
IASNLAALCRPLPGFTRLSPTALAILEEVTQQRHAIKAYPLRYQNAGFHPLTLIKTMQAIIKPDTTLCLDMGSFHIWIAR
YLSCFRARQMLVSNGQQTMGVALPWAIAASLLKPGHKVVSVSGDGGFMQSSMELETAVRLKCNIVHIIWVDNAYNMVEMQ
EVQKYHRAAGVKFGPIDFKAYAESFGARGYAITQPDQLLPTLRQAMEVEGPAVVAIPVDYSDNPKLMQARTEANQDPVFQ
TLEGVFA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=25.354609929078, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI21361361, Length=587, Percent_Identity=25.5536626916525, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1790104, Length=568, Percent_Identity=30.2816901408451, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=27.8745644599303, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786717, Length=533, Percent_Identity=25.328330206379, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787096, Length=535, Percent_Identity=24.4859813084112, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1788716, Length=572, Percent_Identity=22.7272727272727, Blast_Score=132, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=25, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=573, Percent_Identity=24.956369982548, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=593, Percent_Identity=23.7774030354132, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=25.9717314487633, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=25.1533742331288, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=25.4867256637168, Blast_Score=136, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61912; Mature: 61912

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSNIHLRSGAQLIAQQLEAIGVRYVFGIPGAKIDRLFDAIEDTKIQMIPVRHEANGAFM
CCCCEECCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCEEEEEEEEECCCCHHH
AGIMGRLSGRAGVTLATSGPGCGNLVTGVATANSEGDPMIAIGGAVKRDYQQKQTHQSMD
HHHHHHCCCCCCEEEEECCCCCCCHHEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHH
TVSIFRSITKYSAEIQHVDATSEVMANAFRLAESGRQGACFISVPQDVLADKTSSEIIIP
HHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCCEEEC
SAYQPTAEADPDSLQEAVERIQKAQRCVVLLGLHASHQATAEAVSAFLHKTQLPVVGTYQ
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEC
AAGAVDINYYHHFAGRVGLFNNQPGDVLLKEADLIITIGFNPIEYDPELWNSQRCPIVHI
CCCEEEEHHHHHHCCCEECCCCCCCCEEEEECCEEEEECCCCCCCCHHHCCCCCCCEEEE
DIEPAQYQIHYQPCLQIVGNIASNLAALCRPLPGFTRLSPTALAILEEVTQQRHAIKAYP
ECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEECC
LRYQNAGFHPLTLIKTMQAIIKPDTTLCLDMGSFHIWIARYLSCFRARQMLVSNGQQTMG
EEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHEE
VALPWAIAASLLKPGHKVVSVSGDGGFMQSSMELETAVRLKCNIVHIIWVDNAYNMVEMQ
EEHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHEEEEEEEEEEEEEECCCCCHHHHH
EVQKYHRAAGVKFGPIDFKAYAESFGARGYAITQPDQLLPTLRQAMEVEGPAVVAIPVDY
HHHHHHHHCCCEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEECCC
SDNPKLMQARTEANQDPVFQTLEGVFA
CCCCHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQSNIHLRSGAQLIAQQLEAIGVRYVFGIPGAKIDRLFDAIEDTKIQMIPVRHEANGAFM
CCCCEECCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCEEEEEEEEECCCCHHH
AGIMGRLSGRAGVTLATSGPGCGNLVTGVATANSEGDPMIAIGGAVKRDYQQKQTHQSMD
HHHHHHCCCCCCEEEEECCCCCCCHHEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHH
TVSIFRSITKYSAEIQHVDATSEVMANAFRLAESGRQGACFISVPQDVLADKTSSEIIIP
HHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCCEEEC
SAYQPTAEADPDSLQEAVERIQKAQRCVVLLGLHASHQATAEAVSAFLHKTQLPVVGTYQ
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEC
AAGAVDINYYHHFAGRVGLFNNQPGDVLLKEADLIITIGFNPIEYDPELWNSQRCPIVHI
CCCEEEEHHHHHHCCCEECCCCCCCCEEEEECCEEEEECCCCCCCCHHHCCCCCCCEEEE
DIEPAQYQIHYQPCLQIVGNIASNLAALCRPLPGFTRLSPTALAILEEVTQQRHAIKAYP
ECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEECC
LRYQNAGFHPLTLIKTMQAIIKPDTTLCLDMGSFHIWIARYLSCFRARQMLVSNGQQTMG
EEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHEE
VALPWAIAASLLKPGHKVVSVSGDGGFMQSSMELETAVRLKCNIVHIIWVDNAYNMVEMQ
EEHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHEEEEEEEEEEEEEECCCCCHHHHH
EVQKYHRAAGVKFGPIDFKAYAESFGARGYAITQPDQLLPTLRQAMEVEGPAVVAIPVDY
HHHHHHHHCCCEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEECCC
SDNPKLMQARTEANQDPVFQTLEGVFA
CCCCHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]