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The map label for this gene is cobB [H]

Identifier: 227081667

GI number: 227081667

Start: 1569795

End: 1570574

Strand: Reverse

Name: cobB [H]

Synonym: VCM66_1451

Alternate gene names: 227081667

Gene position: 1570574-1569795 (Counterclockwise)

Preceding gene: 227081668

Following gene: 227081664

Centisome position: 54.3

GC content: 50.77

Gene sequence:

>780_bases
GTGTTAAGCTTAAACAAAAGCCGAGAAAAGGAATGGGTTATGAGCTTACCGTATCGACATGTTGTGATACTGACTGGAGC
AGGCATCTCTGCGGAGTCTGGTATTCAAACTTTCCGTGCTCAGGATGGGCTGTGGGAAAACCATCGAATCGAAGATGTGG
CGACACCCGAAGGGTTTCAGCGTGATCCCGATATGGTGTTGGAGTTTTATAACCAGCGCCGCCGTAAGCTACTCTCTGAC
GCTATTCAGCCTAACCCTGCACACCTTGCTCTTGGCAAACTGGAAAAAGAGTTGCAGGGCAGTGTCACTGTGATTACCCA
GAACATTGATAACTTGCACGAACGTGGCGGCAGCCAGAACATTATTCATATGCATGGAGAGCTGCTGAAAGCGCGCTGTC
CTGAGTCCAATCAAACGGTCGAGCAAAAAGAGGATATCCGTCATGGCGATCTGTGCCACTGCTGCCAAATGCCTGCACAG
ATGCGCCCACACATTGTCTGGTTTGGTGAAATGCCGCTACGCATGGGCGATATTTACGCCGCGCTTGAACAAGCAGATCT
GTTTGTGTCTATTGGCACCTCTGGGGTGGTTTATCCTGCGGCGGGGTTTGTGCATGACGCACGTATGCATGGCGCGCACA
CCATAGAGATCAATTTGGAGCCAAGTGCAGTGGAAAGCGAGTTTGCTGAGAAGCGCTATGGTAAAGCCAGTATTGAAGTG
CCAAGGTTAGTGGAAGAAATCTTAGCCGCTCAGGCGCGAGCGAAAGAGAGTGCAGCTTAA

Upstream 100 bases:

>100_bases
TAATACAGGTCTAGCGTTCAACACCCAGCACTCGCTGGGTGTTTTTATCCTTGTTACTACTATGGTCTAATCCCCGTATT
CTAGGGTTGATAACGCTTCG

Downstream 100 bases:

>100_bases
GTGCCGTTGGTTATTTATGTGATGACTTGAGCAAGAATACTTGCATTTAACTCACAGCAACAACAAACGCCCCAAAAACG
GGGCGTTTGTTTAAAGCGAA

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLSLNKSREKEWVMSLPYRHVVILTGAGISAESGIQTFRAQDGLWENHRIEDVATPEGFQRDPDMVLEFYNQRRRKLLSD
AIQPNPAHLALGKLEKELQGSVTVITQNIDNLHERGGSQNIIHMHGELLKARCPESNQTVEQKEDIRHGDLCHCCQMPAQ
MRPHIVWFGEMPLRMGDIYAALEQADLFVSIGTSGVVYPAAGFVHDARMHGAHTIEINLEPSAVESEFAEKRYGKASIEV
PRLVEEILAAQARAKESAA

Sequences:

>Translated_259_residues
MLSLNKSREKEWVMSLPYRHVVILTGAGISAESGIQTFRAQDGLWENHRIEDVATPEGFQRDPDMVLEFYNQRRRKLLSD
AIQPNPAHLALGKLEKELQGSVTVITQNIDNLHERGGSQNIIHMHGELLKARCPESNQTVEQKEDIRHGDLCHCCQMPAQ
MRPHIVWFGEMPLRMGDIYAALEQADLFVSIGTSGVVYPAAGFVHDARMHGAHTIEINLEPSAVESEFAEKRYGKASIEV
PRLVEEILAAQARAKESAA
>Mature_259_residues
MLSLNKSREKEWVMSLPYRHVVILTGAGISAESGIQTFRAQDGLWENHRIEDVATPEGFQRDPDMVLEFYNQRRRKLLSD
AIQPNPAHLALGKLEKELQGSVTVITQNIDNLHERGGSQNIIHMHGELLKARCPESNQTVEQKEDIRHGDLCHCCQMPAQ
MRPHIVWFGEMPLRMGDIYAALEQADLFVSIGTSGVVYPAAGFVHDARMHGAHTIEINLEPSAVESEFAEKRYGKASIEV
PRLVEEILAAQARAKESAA

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=232, Percent_Identity=37.5, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI13787215, Length=238, Percent_Identity=36.5546218487395, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI6912664, Length=250, Percent_Identity=35.6, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI7657575, Length=223, Percent_Identity=30.4932735426009, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI308199517, Length=228, Percent_Identity=65.7894736842105, Blast_Score=326, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI17541892, Length=203, Percent_Identity=30.5418719211823, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28571445, Length=207, Percent_Identity=27.0531400966184, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 28997; Mature: 28997

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLNKSREKEWVMSLPYRHVVILTGAGISAESGIQTFRAQDGLWENHRIEDVATPEGFQ
CCCCCCCCCHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RDPDMVLEFYNQRRRKLLSDAIQPNPAHLALGKLEKELQGSVTVITQNIDNLHERGGSQN
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCC
IIHMHGELLKARCPESNQTVEQKEDIRHGDLCHCCQMPAQMRPHIVWFGEMPLRMGDIYA
EEEHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHCCCHHCCCCEEEECCCCCHHHHHHH
ALEQADLFVSIGTSGVVYPAAGFVHDARMHGAHTIEINLEPSAVESEFAEKRYGKASIEV
HHHHCCEEEEECCCCEEECCCCHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCH
PRLVEEILAAQARAKESAA
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSLNKSREKEWVMSLPYRHVVILTGAGISAESGIQTFRAQDGLWENHRIEDVATPEGFQ
CCCCCCCCCHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RDPDMVLEFYNQRRRKLLSDAIQPNPAHLALGKLEKELQGSVTVITQNIDNLHERGGSQN
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCC
IIHMHGELLKARCPESNQTVEQKEDIRHGDLCHCCQMPAQMRPHIVWFGEMPLRMGDIYA
EEEHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHCCCHHCCCCEEEECCCCCHHHHHHH
ALEQADLFVSIGTSGVVYPAAGFVHDARMHGAHTIEINLEPSAVESEFAEKRYGKASIEV
HHHHCCEEEEECCCCEEECCCCHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCH
PRLVEEILAAQARAKESAA
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]