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The map label for this gene is yebU [H]

Identifier: 227081661

GI number: 227081661

Start: 1563383

End: 1564894

Strand: Direct

Name: yebU [H]

Synonym: VCM66_1445

Alternate gene names: 227081661

Gene position: 1563383-1564894 (Clockwise)

Preceding gene: 227081660

Following gene: 227081663

Centisome position: 54.05

GC content: 48.74

Gene sequence:

>1512_bases
TTGTGCTGCTTTTCTACTTTTCGGCGATCCTTTATGTCACCGCCTTATCTGGTTATAATCGCCGCCCTTATTCATCTACG
GAACTCGCTTGTGCATCCGAACATTTCCCTTCCCGAACCATTCATCGCGTCGATGGCGAAAATACTGCCAGATCCCACTC
AGCTTGCTGATTTTATTGCAGCTTGCCAAAGGCCTCTGCGCAAAAGTATTCGCGTTAATACACTCAAAATTTCTGTCGCA
GAGTTTTGTCAGCGCGCCGCTGAGAAAGAGTGGCAACTGACGCCAGTACCTTGGTGTGAGAATGGGTTTTGGATTGATGC
CGATGAAAGCTTAGTCCCGCTGGGTAATACCGCTGAGCATATGGCTGGGCTGTTTTATATCCAAGAAGCCAGTTCGATGA
TGCCCGTTTCAGCCCTGTTTATGGGGAATGCGCACTATGACTCTGTGTTGGATATGGCGGCGGCTCCGGGCTCGAAAACC
ACGCAAATGGCCGCCTTGATGGACAACCAAGGCGTGTTGGTTGCCAATGAGTTTTCCGCAAGCCGAGTGAAAGTGCTGCA
CGCCAACATTGAGCGCTGTGGGATTCGTAATACCGCACTAAGTAATTTTGATGGTTGTGTGTTTGGAGGTTGGTTGCCTG
AGCGTTTTGATGCGGTGTTGATCGACGCGCCCTGCTCTGGTGAAGGTACAATTCGCAAAGATCCTGATGCGATGAAAAAC
TGGAGCCTTGAGTCGATTCAAAGTATTGCCAACACTCAAAAAGCCTTAATTGAAAGCGCATTTCAGGCGCTCAAAGTGGG
CGGCACGCTGGTGTATTCAACCTGTACCTTGAGCCGTGAAGAAAATCAGCAAGTGTGCTGGCACTTAAAGCAGACATATG
GTGATGCGGTTAGCTTTGAATCACTGGGTAATTTATTTGAGCACGCATCGCTCGCCCTTACCGAAGAAGGCTTCCTGCAC
ATTTTTCCGCAAATGTACGATTGTGAAGGCTTTTTTGTCGCGAAAATTCGTAAACTAGCCTCCGTGCCAACGCCAGAGGT
CAACAAGCGATTAGGCAAGTTTCCGTTTAACAAAGCGAGCCATAAGCAGCAAGCGGAGATTGCTCAGCAACTTCACAAGT
CTCTTGGTATCGAGCTGCCCTCGGATGCACAGGTCTGGTTGCGCGATAATGATGTGTGGCTATTCCCCGAAGCTTTAGAG
CCCTTATTGGGTGAGCTACGCTTTTCTCGCATGGGAATAAAAATTGCTGAAGCACATAAATCGGGTTATCGATGGCAGCA
TCAAGTGGCGACTTGTCTTGCATCCTCAAGTGCGAGTCACAGTGTCGAGCTGGATACCACGCAAGCACGAGAATGGTTTA
TGGGCAGAGATGTCAGACCAGAAGGACAAAGTGGTCAAGGCGAAGTGATCATCCGTTACGCTAACGATGTGATTGGGTTA
GGAAAATGGGTAGGCAATCGCGTGAAGAATGGACTGCCAAGAGAGCTAGTTAGAGACAAAAATCTATTTTGA

Upstream 100 bases:

>100_bases
TACTTTCTACCTCTACCCTCAAGCCCAAGAGGAATGGACCAAGTGGCGCACACCGATCCCTAAGCCTTAGTCGCAAAAGA
TAACGTGAAGAAAAGCAGCC

Downstream 100 bases:

>100_bases
TTGGCTGAACTATAGTATTAAATTCTAAAAACCTGTCGCTAATTTCAGTATCTGTACTAGGCTTAGAACTAAGCAACTGA
CTAGATTTTCCATTAGCGCA

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MCCFSTFRRSFMSPPYLVIIAALIHLRNSLVHPNISLPEPFIASMAKILPDPTQLADFIAACQRPLRKSIRVNTLKISVA
EFCQRAAEKEWQLTPVPWCENGFWIDADESLVPLGNTAEHMAGLFYIQEASSMMPVSALFMGNAHYDSVLDMAAAPGSKT
TQMAALMDNQGVLVANEFSASRVKVLHANIERCGIRNTALSNFDGCVFGGWLPERFDAVLIDAPCSGEGTIRKDPDAMKN
WSLESIQSIANTQKALIESAFQALKVGGTLVYSTCTLSREENQQVCWHLKQTYGDAVSFESLGNLFEHASLALTEEGFLH
IFPQMYDCEGFFVAKIRKLASVPTPEVNKRLGKFPFNKASHKQQAEIAQQLHKSLGIELPSDAQVWLRDNDVWLFPEALE
PLLGELRFSRMGIKIAEAHKSGYRWQHQVATCLASSSASHSVELDTTQAREWFMGRDVRPEGQSGQGEVIIRYANDVIGL
GKWVGNRVKNGLPRELVRDKNLF

Sequences:

>Translated_503_residues
MCCFSTFRRSFMSPPYLVIIAALIHLRNSLVHPNISLPEPFIASMAKILPDPTQLADFIAACQRPLRKSIRVNTLKISVA
EFCQRAAEKEWQLTPVPWCENGFWIDADESLVPLGNTAEHMAGLFYIQEASSMMPVSALFMGNAHYDSVLDMAAAPGSKT
TQMAALMDNQGVLVANEFSASRVKVLHANIERCGIRNTALSNFDGCVFGGWLPERFDAVLIDAPCSGEGTIRKDPDAMKN
WSLESIQSIANTQKALIESAFQALKVGGTLVYSTCTLSREENQQVCWHLKQTYGDAVSFESLGNLFEHASLALTEEGFLH
IFPQMYDCEGFFVAKIRKLASVPTPEVNKRLGKFPFNKASHKQQAEIAQQLHKSLGIELPSDAQVWLRDNDVWLFPEALE
PLLGELRFSRMGIKIAEAHKSGYRWQHQVATCLASSSASHSVELDTTQAREWFMGRDVRPEGQSGQGEVIIRYANDVIGL
GKWVGNRVKNGLPRELVRDKNLF
>Mature_503_residues
MCCFSTFRRSFMSPPYLVIIAALIHLRNSLVHPNISLPEPFIASMAKILPDPTQLADFIAACQRPLRKSIRVNTLKISVA
EFCQRAAEKEWQLTPVPWCENGFWIDADESLVPLGNTAEHMAGLFYIQEASSMMPVSALFMGNAHYDSVLDMAAAPGSKT
TQMAALMDNQGVLVANEFSASRVKVLHANIERCGIRNTALSNFDGCVFGGWLPERFDAVLIDAPCSGEGTIRKDPDAMKN
WSLESIQSIANTQKALIESAFQALKVGGTLVYSTCTLSREENQQVCWHLKQTYGDAVSFESLGNLFEHASLALTEEGFLH
IFPQMYDCEGFFVAKIRKLASVPTPEVNKRLGKFPFNKASHKQQAEIAQQLHKSLGIELPSDAQVWLRDNDVWLFPEALE
PLLGELRFSRMGIKIAEAHKSGYRWQHQVATCLASSSASHSVELDTTQAREWFMGRDVRPEGQSGQGEVIIRYANDVIGL
GKWVGNRVKNGLPRELVRDKNLF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=324, Percent_Identity=35.4938271604938, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI76150623, Length=324, Percent_Identity=35.4938271604938, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI32698918, Length=158, Percent_Identity=37.3417721518987, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI11545785, Length=224, Percent_Identity=32.5892857142857, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI301336155, Length=275, Percent_Identity=27.6363636363636, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI39995082, Length=202, Percent_Identity=29.7029702970297, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270288816, Length=273, Percent_Identity=27.1062271062271, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI23199998, Length=233, Percent_Identity=28.3261802575107, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI8922322, Length=233, Percent_Identity=28.3261802575107, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI270288818, Length=233, Percent_Identity=28.3261802575107, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40316918, Length=219, Percent_Identity=30.5936073059361, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI87081985, Length=472, Percent_Identity=56.5677966101695, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI2367212, Length=217, Percent_Identity=31.3364055299539, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=316, Percent_Identity=33.2278481012658, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25143471, Length=175, Percent_Identity=36, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71998419, Length=303, Percent_Identity=26.4026402640264, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=228, Percent_Identity=27.6315789473684, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17509941, Length=163, Percent_Identity=31.9018404907975, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=362, Percent_Identity=35.0828729281768, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=196, Percent_Identity=33.1632653061224, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI22024126, Length=340, Percent_Identity=33.5294117647059, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI21355201, Length=256, Percent_Identity=32.8125, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24668781, Length=159, Percent_Identity=35.8490566037736, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356513, Length=159, Percent_Identity=35.8490566037736, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 55998; Mature: 55998

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCCFSTFRRSFMSPPYLVIIAALIHLRNSLVHPNISLPEPFIASMAKILPDPTQLADFIA
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
ACQRPLRKSIRVNTLKISVAEFCQRAAEKEWQLTPVPWCENGFWIDADESLVPLGNTAEH
HHHHHHHHHHEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCEECCCCCHHH
MAGLFYIQEASSMMPVSALFMGNAHYDSVLDMAAAPGSKTTQMAALMDNQGVLVANEFSA
HHHHHHEEHHCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCC
SRVKVLHANIERCGIRNTALSNFDGCVFGGWLPERFDAVLIDAPCSGEGTIRKDPDAMKN
CCEEEEEHHHHHCCCCHHHHCCCCCCEECCCCHHCCCEEEEECCCCCCCCCCCCCHHHHC
WSLESIQSIANTQKALIESAFQALKVGGTLVYSTCTLSREENQQVCWHLKQTYGDAVSFE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHH
SLGNLFEHASLALTEEGFLHIFPQMYDCEGFFVAKIRKLASVPTPEVNKRLGKFPFNKAS
HHHHHHHHHHHEEECCCCEEECCHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCC
HKQQAEIAQQLHKSLGIELPSDAQVWLRDNDVWLFPEALEPLLGELRFSRMGIKIAEAHK
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEECHHHHHHHHHHHHHHHCCCEEEHHHC
SGYRWQHQVATCLASSSASHSVELDTTQAREWFMGRDVRPEGQSGQGEVIIRYANDVIGL
CCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHH
GKWVGNRVKNGLPRELVRDKNLF
HHHHHHHHHCCCCHHHHHCCCCC
>Mature Secondary Structure
MCCFSTFRRSFMSPPYLVIIAALIHLRNSLVHPNISLPEPFIASMAKILPDPTQLADFIA
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
ACQRPLRKSIRVNTLKISVAEFCQRAAEKEWQLTPVPWCENGFWIDADESLVPLGNTAEH
HHHHHHHHHHEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCEECCCCCHHH
MAGLFYIQEASSMMPVSALFMGNAHYDSVLDMAAAPGSKTTQMAALMDNQGVLVANEFSA
HHHHHHEEHHCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCC
SRVKVLHANIERCGIRNTALSNFDGCVFGGWLPERFDAVLIDAPCSGEGTIRKDPDAMKN
CCEEEEEHHHHHCCCCHHHHCCCCCCEECCCCHHCCCEEEEECCCCCCCCCCCCCHHHHC
WSLESIQSIANTQKALIESAFQALKVGGTLVYSTCTLSREENQQVCWHLKQTYGDAVSFE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHH
SLGNLFEHASLALTEEGFLHIFPQMYDCEGFFVAKIRKLASVPTPEVNKRLGKFPFNKAS
HHHHHHHHHHHEEECCCCEEECCHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCC
HKQQAEIAQQLHKSLGIELPSDAQVWLRDNDVWLFPEALEPLLGELRFSRMGIKIAEAHK
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEECHHHHHHHHHHHHHHHCCCEEEHHHC
SGYRWQHQVATCLASSSASHSVELDTTQAREWFMGRDVRPEGQSGQGEVIIRYANDVIGL
CCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHH
GKWVGNRVKNGLPRELVRDKNLF
HHHHHHHHHCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]