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The map label for this gene is rlmL

Identifier: 227081647

GI number: 227081647

Start: 1541016

End: 1543142

Strand: Reverse

Name: rlmL

Synonym: VCM66_1431

Alternate gene names: 227081647

Gene position: 1543142-1541016 (Counterclockwise)

Preceding gene: 227081648

Following gene: 227081646

Centisome position: 53.35

GC content: 46.26

Gene sequence:

>2127_bases
ATGAATCAGTATCTAGCGGTGACGTCTAACGGCCTTGAGAATCTATTAGTTGAAGAATTAACCCAACTGGGTATTAACGA
TGCCAAACCCGTTCAGGCCGGGGTTAAATTTAAAGCAACCAATGAACAGATCTATCGCTGCTGTCTATGGAGTCGTCTCG
CATCAAGATTTGTTCGTATTGTGGCTGAATTTAAGTGTCAGAATGACTTAGATCTCTATCTTTCAACAACTTCAGTCAAC
TGGGTCAACTATTTCCACAGTTCTAAAAAGCTCGTTGTTGATTTCAATGGCACGAACCGAGAAATTCGTAATAGCCAATA
CGGCGCGATGAAAGTCAAAGACGCCATCGTAGATTGCTTCACTAAGAAAAATCTTCCGCGTCCGTCGATCAGCAAAGATC
TTGCGGATCTTCATATCCATGTCCGCTTACACAAAGAGAATGCTCTGCTTGGTATCGATATGGTGGGTTCAGGCTTGCAT
GCTCGGGGCTACCGAACTGAAGCAGGTAAAGCGCCATTACGTGAAACATTAGCCGCCGCAATCATTCTGCGCAGTGGTTG
GGATGCCAGTAAACCGCTTCTCGATCCTATGTGTGGCTCCGGTACCTTACTGATTGAAGCGGCGATGATGGCTGCCAATA
TTGCTCCAGGCTTACAACGTAAAAAGTGGGGCTTCGAAGCATTAGAAGATTTCGAACCAGAACTCTGGGCAAGTGTGAAA
TCAGAAGCCAGCGTGCAAGGTAAACGTGGAGTCAAAAAGGTAGAAACTCATTTCTACGGCGTAGATAACGATAACCGCGT
ATTGCAGACGGCTAAAGATAACGCCCGCCGTGCCGGTGTAGAAGAGCTAATCAGCTTTACCTTGGGTGATGCGGCAAAAG
TAAAACGCCCAGAAAACTTCGCTGAAGGTATTGTGATTTGTAACCCACCGTACGGTGAGCGTTTAGGCACTCATCCCGGT
TTGATCGCTCTTTACACTGCATTTGGTGCACAGCTTAAAGCTGAGTTTGGTGGTTGCCACGCTTCTATTTTCTCGAGTTC
TGACGAATTACTCAGTTGTTTGCGCATGCGTGCGGACAAACAGTTCAAGCTGAATAATGGTGCGTTACCATGTCACCAAA
AAAATTACACCATAGCCATGCGAGAGCAAAACAGCGTCAGCAATGAAGGTACTCAAGAAATCCTCATTGCCCCCGATTTT
GCCAACCGTTTGAAGAAAAACTTCAACAAGATTGGCAAATGGGCTAAGCGTGAGGGGCTCGATTGCTTCCGCCTCTATGA
TGCGGATCTTCCCGAATACAATGTGGCTATTGATGTCTACCAAGATCATCTCATGATCCAAGAATATGCAGCGCCAAAGG
ATATTCCTGAAGAGAAAGCGAAGCGTCGTTTGACCGATATTATTCGCGCAGCCATTCAAGTGTTGGATGTCGATGCGAAC
AACGTCGTGCTCAAAGTTCGTGAAAGACAAAAAGGCACGTCGCAATACGAGAAACTCGGCCAGCAAGCACAGACCATGCA
AATTACCGAGTATGGCGTGAAGTTGATCGTCAACCTATACGATTATCTCGATACTGGCTTATTCCTTGACCACAAAATTA
CTCGTCGTCGTTTAGGGCAAATGGCACAAGGCAAAGATTTTCTCAATTTATTTGCCTATACCGGCTCTGCGACAGTACAT
GCTGCTTGTGGCGGTGCAAAATCGACGACGACCGTCGATATGTCGAAAACTTATCTTGAATGGGCCAAAGAGAACATGCA
GTTAAACGGGCAGGTTGGGCGTCAGCACCAGTACGTTCAGGCCGACTGTCTACAGTGGCTCGCGAATGCCCAAAGCCAGT
ACGATCTTATCTTTATTGATCCGCCAACGTTTTCTAACTCTAAACGTATGGAGCAGACTTTTGATGTGCAACGTGACCAC
GTCACGCTTATGACCAATCTTAAACGGTTGCTGCGCCCAGAAGGGACGATTGTTTTCTCTAATAACAAGCGCCATTTCAA
GATGGATATGGAAGCGCTACACGCTTTGGGCCTCAACGCTCAAAATATCTCGCATCAAACCTTACCGCTGGATTTTGAGC
GAAATAAACAAATTCATAACTGCTGGCTGATCACCCATCAGAGTTAG

Upstream 100 bases:

>100_bases
GATAAAAGAGAGATTTTTTGGCTTGGTGATGAGTTAGCTTTAGGTATGGTTCACATTGTCAGTTATAATCTGAGACCATT
TTTATCAGCAAAAGAACACT

Downstream 100 bases:

>100_bases
GAAATTCAATGATAACCTTGTACAGCACTGAAGGATGCCATCTCTGTGAGATGGCCTATGCGTTACTCAATGAAGTAGGG
CTCACCAAACAAGTTGACGT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MNQYLAVTSNGLENLLVEELTQLGINDAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRIVAEFKCQNDLDLYLSTTSVN
WVNYFHSSKKLVVDFNGTNREIRNSQYGAMKVKDAIVDCFTKKNLPRPSISKDLADLHIHVRLHKENALLGIDMVGSGLH
ARGYRTEAGKAPLRETLAAAIILRSGWDASKPLLDPMCGSGTLLIEAAMMAANIAPGLQRKKWGFEALEDFEPELWASVK
SEASVQGKRGVKKVETHFYGVDNDNRVLQTAKDNARRAGVEELISFTLGDAAKVKRPENFAEGIVICNPPYGERLGTHPG
LIALYTAFGAQLKAEFGGCHASIFSSSDELLSCLRMRADKQFKLNNGALPCHQKNYTIAMREQNSVSNEGTQEILIAPDF
ANRLKKNFNKIGKWAKREGLDCFRLYDADLPEYNVAIDVYQDHLMIQEYAAPKDIPEEKAKRRLTDIIRAAIQVLDVDAN
NVVLKVRERQKGTSQYEKLGQQAQTMQITEYGVKLIVNLYDYLDTGLFLDHKITRRRLGQMAQGKDFLNLFAYTGSATVH
AACGGAKSTTTVDMSKTYLEWAKENMQLNGQVGRQHQYVQADCLQWLANAQSQYDLIFIDPPTFSNSKRMEQTFDVQRDH
VTLMTNLKRLLRPEGTIVFSNNKRHFKMDMEALHALGLNAQNISHQTLPLDFERNKQIHNCWLITHQS

Sequences:

>Translated_708_residues
MNQYLAVTSNGLENLLVEELTQLGINDAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRIVAEFKCQNDLDLYLSTTSVN
WVNYFHSSKKLVVDFNGTNREIRNSQYGAMKVKDAIVDCFTKKNLPRPSISKDLADLHIHVRLHKENALLGIDMVGSGLH
ARGYRTEAGKAPLRETLAAAIILRSGWDASKPLLDPMCGSGTLLIEAAMMAANIAPGLQRKKWGFEALEDFEPELWASVK
SEASVQGKRGVKKVETHFYGVDNDNRVLQTAKDNARRAGVEELISFTLGDAAKVKRPENFAEGIVICNPPYGERLGTHPG
LIALYTAFGAQLKAEFGGCHASIFSSSDELLSCLRMRADKQFKLNNGALPCHQKNYTIAMREQNSVSNEGTQEILIAPDF
ANRLKKNFNKIGKWAKREGLDCFRLYDADLPEYNVAIDVYQDHLMIQEYAAPKDIPEEKAKRRLTDIIRAAIQVLDVDAN
NVVLKVRERQKGTSQYEKLGQQAQTMQITEYGVKLIVNLYDYLDTGLFLDHKITRRRLGQMAQGKDFLNLFAYTGSATVH
AACGGAKSTTTVDMSKTYLEWAKENMQLNGQVGRQHQYVQADCLQWLANAQSQYDLIFIDPPTFSNSKRMEQTFDVQRDH
VTLMTNLKRLLRPEGTIVFSNNKRHFKMDMEALHALGLNAQNISHQTLPLDFERNKQIHNCWLITHQS
>Mature_708_residues
MNQYLAVTSNGLENLLVEELTQLGINDAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRIVAEFKCQNDLDLYLSTTSVN
WVNYFHSSKKLVVDFNGTNREIRNSQYGAMKVKDAIVDCFTKKNLPRPSISKDLADLHIHVRLHKENALLGIDMVGSGLH
ARGYRTEAGKAPLRETLAAAIILRSGWDASKPLLDPMCGSGTLLIEAAMMAANIAPGLQRKKWGFEALEDFEPELWASVK
SEASVQGKRGVKKVETHFYGVDNDNRVLQTAKDNARRAGVEELISFTLGDAAKVKRPENFAEGIVICNPPYGERLGTHPG
LIALYTAFGAQLKAEFGGCHASIFSSSDELLSCLRMRADKQFKLNNGALPCHQKNYTIAMREQNSVSNEGTQEILIAPDF
ANRLKKNFNKIGKWAKREGLDCFRLYDADLPEYNVAIDVYQDHLMIQEYAAPKDIPEEKAKRRLTDIIRAAIQVLDVDAN
NVVLKVRERQKGTSQYEKLGQQAQTMQITEYGVKLIVNLYDYLDTGLFLDHKITRRRLGQMAQGKDFLNLFAYTGSATVH
AACGGAKSTTTVDMSKTYLEWAKENMQLNGQVGRQHQYVQADCLQWLANAQSQYDLIFIDPPTFSNSKRMEQTFDVQRDH
VTLMTNLKRLLRPEGTIVFSNNKRHFKMDMEALHALGLNAQNISHQTLPLDFERNKQIHNCWLITHQS

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=706, Percent_Identity=57.0821529745043, Blast_Score=841, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=241, Percent_Identity=27.8008298755187, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_VIBC3 (A5F836)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F836
- SMR:   A5F836
- STRING:   A5F836
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1095
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   VCHO345073:VC0395_A1095-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 79834; Mature: 79834

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYLAVTSNGLENLLVEELTQLGINDAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRI
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
VAEFKCQNDLDLYLSTTSVNWVNYFHSSKKLVVDFNGTNREIRNSQYGAMKVKDAIVDCF
HHHHCCCCCCEEEEEECEEHHHHHHCCCCEEEEEECCCCCHHCCCCCCCHHHHHHHHHHH
TKKNLPRPSISKDLADLHIHVRLHKENALLGIDMVGSGLHARGYRTEAGKAPLRETLAAA
HHCCCCCCCHHHCCEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
IILRSGWDASKPLLDPMCGSGTLLIEAAMMAANIAPGLQRKKWGFEALEDFEPELWASVK
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCCHHHHHHHH
SEASVQGKRGVKKVETHFYGVDNDNRVLQTAKDNARRAGVEELISFTLGDAAKVKRPENF
HHCCCCHHCCHHHHHHEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHH
AEGIVICNPPYGERLGTHPGLIALYTAFGAQLKAEFGGCHASIFSSSDELLSCLRMRADK
HCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCCC
QFKLNNGALPCHQKNYTIAMREQNSVSNEGTQEILIAPDFANRLKKNFNKIGKWAKREGL
CEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCC
DCFRLYDADLPEYNVAIDVYQDHLMIQEYAAPKDIPEEKAKRRLTDIIRAAIQVLDVDAN
CEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
NVVLKVRERQKGTSQYEKLGQQAQTMQITEYGVKLIVNLYDYLDTGLFLDHKITRRRLGQ
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHH
MAQGKDFLNLFAYTGSATVHAACGGAKSTTTVDMSKTYLEWAKENMQLNGQVGRQHQYVQ
HHCCCHHHHHEEECCCCEEEEECCCCCCCEEEEHHHHHHHHHHHCCEECCCCCCCHHHHH
ADCLQWLANAQSQYDLIFIDPPTFSNSKRMEQTFDVQRDHVTLMTNLKRLLRPEGTIVFS
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEC
NNKRHFKMDMEALHALGLNAQNISHQTLPLDFERNKQIHNCWLITHQS
CCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MNQYLAVTSNGLENLLVEELTQLGINDAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRI
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
VAEFKCQNDLDLYLSTTSVNWVNYFHSSKKLVVDFNGTNREIRNSQYGAMKVKDAIVDCF
HHHHCCCCCCEEEEEECEEHHHHHHCCCCEEEEEECCCCCHHCCCCCCCHHHHHHHHHHH
TKKNLPRPSISKDLADLHIHVRLHKENALLGIDMVGSGLHARGYRTEAGKAPLRETLAAA
HHCCCCCCCHHHCCEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
IILRSGWDASKPLLDPMCGSGTLLIEAAMMAANIAPGLQRKKWGFEALEDFEPELWASVK
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCCHHHHHHHH
SEASVQGKRGVKKVETHFYGVDNDNRVLQTAKDNARRAGVEELISFTLGDAAKVKRPENF
HHCCCCHHCCHHHHHHEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHH
AEGIVICNPPYGERLGTHPGLIALYTAFGAQLKAEFGGCHASIFSSSDELLSCLRMRADK
HCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCCC
QFKLNNGALPCHQKNYTIAMREQNSVSNEGTQEILIAPDFANRLKKNFNKIGKWAKREGL
CEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCC
DCFRLYDADLPEYNVAIDVYQDHLMIQEYAAPKDIPEEKAKRRLTDIIRAAIQVLDVDAN
CEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
NVVLKVRERQKGTSQYEKLGQQAQTMQITEYGVKLIVNLYDYLDTGLFLDHKITRRRLGQ
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHH
MAQGKDFLNLFAYTGSATVHAACGGAKSTTTVDMSKTYLEWAKENMQLNGQVGRQHQYVQ
HHCCCHHHHHEEECCCCEEEEECCCCCCCEEEEHHHHHHHHHHHCCEECCCCCCCHHHHH
ADCLQWLANAQSQYDLIFIDPPTFSNSKRMEQTFDVQRDHVTLMTNLKRLLRPEGTIVFS
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEC
NNKRHFKMDMEALHALGLNAQNISHQTLPLDFERNKQIHNCWLITHQS
CCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA