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The map label for this gene is rmf [H]

Identifier: 227081643

GI number: 227081643

Start: 1536828

End: 1537001

Strand: Reverse

Name: rmf [H]

Synonym: VCM66_1427

Alternate gene names: 227081643

Gene position: 1537001-1536828 (Counterclockwise)

Preceding gene: 227081644

Following gene: 227081640

Centisome position: 53.14

GC content: 45.4

Gene sequence:

>174_bases
ATGAAGAGACAAAAGCGTGATCGCCTAGAAAGAGCTCAATCGCAAGGATACAAAGCCGGTCTAAACGGTCGATCCCATGA
CGAATGTCCCTATCAACAGACGGAAGTACGCTCTTATTGGTTAGGTGGTTGGCGTGATGCTAGAAACGATAAACTTTCTG
GTCTCTGCAAATAA

Upstream 100 bases:

>100_bases
GGCGAATAATTGATCGATTCTGTATTGGTGTGGTCATAAAAGATCCACACAAGTTTAATAGTTGTACGTTACACCAAAGT
TACCGTATGAGGACAGAACT

Downstream 100 bases:

>100_bases
TTTCCCATACAACAAAGCTTGAAAAGGCTTATCGCTAGGAAAACAGCCCGAAACGGGCTGTTATTCGTTAGAGATTCTAT
CTTTAAAACGCAGACGTATC

Product: ribosome modulation factor

Products: NA

Alternate protein names: Protein E [H]

Number of amino acids: Translated: 57; Mature: 57

Protein sequence:

>57_residues
MKRQKRDRLERAQSQGYKAGLNGRSHDECPYQQTEVRSYWLGGWRDARNDKLSGLCK

Sequences:

>Translated_57_residues
MKRQKRDRLERAQSQGYKAGLNGRSHDECPYQQTEVRSYWLGGWRDARNDKLSGLCK
>Mature_57_residues
MKRQKRDRLERAQSQGYKAGLNGRSHDECPYQQTEVRSYWLGGWRDARNDKLSGLCK

Specific function: Associates with 70S ribosomes and converts them to a dimeric form (100S ribosomes) which appears during the transition from the exponential growth phase to the stationary phase of E.coli cells [H]

COG id: COG3130

COG function: function code J; Ribosome modulation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787186, Length=52, Percent_Identity=63.4615384615385, Blast_Score=72, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007040
- InterPro:   IPR023200
- ProDom:   PD051650 [H]

Pfam domain/function: PF04957 RMF [H]

EC number: NA

Molecular weight: Translated: 6729; Mature: 6729

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQKRDRLERAQSQGYKAGLNGRSHDECPYQQTEVRSYWLGGWRDARNDKLSGLCK
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRQKRDRLERAQSQGYKAGLNGRSHDECPYQQTEVRSYWLGGWRDARNDKLSGLCK
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]